Factors Affecting the Oxygen Tension around Cells Growing in Plastic Petri Dishes

Chapman, J. D.; Sturrock, J.E.; Boag, J. W.; Crookall, J. O.

doi:10.1080/09553007014550381

Cited by 81 publications

(34 citation statements)

References 14 publications

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“…There are also recoil target nuclei and the fragments from these. We estimate the overall dose contributions from these are small, a few percent at most; however, at some future time it might be important to assess the local effects of multi- slowly by diffusion (54). At a given measured dose, the x-ray survival measured for cells irradiated on glass was considerably less than for cells irradiated on plastic because of scattered secondary electrons.…”

Section: Heavy-ion Dosimetrymentioning

confidence: 97%

Inactivation of Human Kidney Cells by High-Energy Monoenergetic Heavy-Ion Beams

Blakely¹,

Tobias²,

Yang³

et al. 1979

Radiation Research

193

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Section: Heavy-ion Dosimetrymentioning

confidence: 97%

Inactivation of Human Kidney Cells by High-Energy Monoenergetic Heavy-Ion Beams

Blakely¹,

Tobias²,

Yang³

et al. 1979

Radiation Research

193

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“…It is not easy to maintain low oxygen atmospheres for long periods of time because of diffusion of oxygen between chambers and the atmosphere and/or problems related to dissolved 02 in plastic materials (Chapman et al, 1970;Richter et al, 1972;Bradley et al, 1978). Therefore, in the current studies considerable amount of time was devoted to maintenance of the proper range of 02 concentrations by measuring 02 in chambers used with a mass spectrometer.…”

Section: Discussionmentioning

confidence: 99%

Modulation of tumour cell colony growth in soft agar by oxygen and its mechanism

Gupta¹,

Eberle²

1984

Br J Cancer

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Summary A simple technique for maintaining low oxygen concentrations (0.1-20%) is described. These conditions were then used to study the effect of oxygen on colony growth of neoplastic cells in soft-agar. Physiologically low oxygen concentrations (0.1-10%) compared to 20%O2 were found to enhance plating efficiency and colony size of tumour cells. The optimal oxygen concentration for plating efficiency varied with tumour studied and may be as low as 0.1%. Having established that tumour cell colonies will grow better at 0.1-10% 02 compared to 20% 02, the mechanism by which this enhancement occurs was investigated. Observations on the effect of free radical scavengers and superoxide dismutase on plating efficiency of Ehrlich's ascites tumour cells suggests that this phenomenon occurs through oxygen toxicity mediated by superoxide anion.

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“…For PpIX generation under hypoxic conditions, cells were plated onto glass Petri dishes coated with type IV collagen solution (30 ,ug ml-', Sigma Chemicals, UK) to prevent oxygen dissolved in the standard plastic tissue culture vessels diffusing into the media during the experiments (Chapman et al, 1970 PpIX estimation This method has been previously published (Wyld et al, 1997). Briefly, exponentially growing tumour cells were incubated with 1 mM ALA in complete media for 4 h in the dark at 37°C.…”

Section: Cell Culture Techniquesmentioning

confidence: 99%

The influence of hypoxia and pH on aminolaevulinic acid-induced photodynamic therapy in bladder cancer cells in vitro

Wyld

Reed²,

Brown³

1998

Br J Cancer

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Summary Photodynamic therapy (PDT) is a cancer treatment based on the interaction of light and a photosensitizing chemical. The photosensitizer protoporphyrin IX (PpIX) is generated via the haem biosynthetic pathway after administration of aminolaevulinic acid (ALA). The cellular microenvironment of tumours is hypoxic and acidotic relative to normal tissue, which may influence PpIX generation and compromise PDT efficacy. This study used bladder cancer cells, incubated with ALA at various oxygen tensions and H+ ion concentrations, and assessed the effects on PpIX generation and PDT sensitivity. PpIX production was reduced at 0%, 2.5% (19 mmHg) and 5% (38 mmHg) oxygen compared with that at 21% (160 mmHg) oxygen (0.15, 0.28 and 0.398 ng g-1 protein compared with 0.68 ng jg-' respectively; P < 0.05). The response to PDT was abolished by hypoxia, as a result of both reduced PpIX synthesis and reduced PDT toxicity. PpIX production was greater at pH 7.0 and 6.5 (0.75 and 0.66 ng gg-1) compared with that at pH 7.4 and 5.5 (0.41 and 0.55 ng jig-1 respectively).PDT cytotoxicity was enhanced at lower pH values. These results suggest that ALA-induced PDT may be inhibited by hypoxia due to reduced intrinsic PpIX synthesis. Acidosis may slightly enhance the efficacy of ALA-induced PDT.

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Factors Affecting the Oxygen Tension around Cells Growing in Plastic Petri Dishes

Cited by 81 publications

References 14 publications

Inactivation of Human Kidney Cells by High-Energy Monoenergetic Heavy-Ion Beams

Inactivation of Human Kidney Cells by High-Energy Monoenergetic Heavy-Ion Beams

Modulation of tumour cell colony growth in soft agar by oxygen and its mechanism

The influence of hypoxia and pH on aminolaevulinic acid-induced photodynamic therapy in bladder cancer cells in vitro

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