Factors affecting the stability of cryogenically preserved granulocytes

Lionetti, Fabian J.; Luscinskas, F L; Hunt, Stephen; Valeri, C. R.; Callahan, Arthur B.

doi:10.1016/0011-2240(80)90037-1

Cited by 14 publications

(3 citation statements)

References 12 publications

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“…HSA, plasma or serum are also added as extracellular cryoprotectants. 32 Recently, the cryopreservation of PBPC with the plasma expander oxypolygelatine instead of human serum protein has been shown to be associated with optimal post-thawing progenitor cell recovery and marrow reconstituting function. 33,34 In the present study, we report our experience of cryopreserving mobilized blood in DMSO and HSA using an uncontrolled-rate freezing procedure followed by storage in a mechanical freezer (−140°C).…”

Section: Discussionmentioning

confidence: 99%

Primitive hematopoietic progenitors within mobilized blood are spared by uncontrolled rate freezing

Cilloni

Garau

Regazzi

et al. 1999

Bone Marrow Transplant

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Summary:Uncontrolled-rate freezing techniques represent an attractive alternative to controlled-rate cryopreservation procedures which are time-consuming and require high-level technical expertise. In this study, we report our experience using uncontrolled-rate cryopreservation and mechanical freezer storage at −140؇C. /l white blood cells and 50 ؋ 10 9 /l platelets were 9 and 13, respectively. In conclusion, the procedure described here is characterized by short execution time, allows a substantial recovery of primitive and committed progenitors and is associated with prompt hematopoietic recovery following myeloablative therapy even after long-term storage.

show abstract

Section: Discussionmentioning

confidence: 99%

Primitive hematopoietic progenitors within mobilized blood are spared by uncontrolled rate freezing

Cilloni

Garau

Regazzi

et al. 1999

Bone Marrow Transplant

View full text Add to dashboard Cite

show abstract

“…This study and previous studies of granulocytes (1,2,3,4) and monocytes (10) volved. These data show that superoxide anion synthesis is a membrane phenomenon (12) distinct from particle ingestion which is more vulnerable to preservation injury.…”

Section: Discussionmentioning

confidence: 99%

“…Leukapheresed dog blood was obtained and processed as described earlier by Lionetti et al (1). Briefly, 100 ml of leukapheresed dog blood was subjected to counterflow centrifugation as described (5) …”

Section: Methodsmentioning

confidence: 99%