Factors contributing to in vitro shoot-tip necrosis and their physiological interactions

Bairu, Michael W.; Stirk, Wendy A.; Staden, J. Van

doi:10.1007/s11240-009-9560-8

Cited by 70 publications

(42 citation statements)

References 67 publications

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“…-no significant differences; (-) -no regeneration or died during storage Several authors ascribed necrosis and browning during cold storage to the presence of light and found them to be less pronounced in darkness (Hausman et al 1994;Romano, Martins-Loução 1999) or when BAP was lacking in the storage medium (Orlikowska 1992). Furthermore, Bairu et al (2009) supposed that when plants are cultured in a cytokinin-free medium, cytokinins are depleted from the shoots; the absence of roots decreases the endogenous cytokinin level, stopping cell division and causing apical necrosis. However, this interpretation may not explain our cold chamber results.…”

Section: Resultsmentioning

confidence: 99%

In vitro storage of plum germplasm by slow growth

Giannì¹,

Sottile²

2015

Hortic. Sci.

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Gianní S., Sottile F. (2015): In vitro storage of plum germplasm by slow growth. Hort. Sci. (Prague), 42: 61-69.In this study, in vitro slow growth storage was investigated in four cultivars of two Sicilian (Southern Italy) plum species (Prunus domestica L. and Prunus cerasifera Ehrh. -two genotypes each). Established shoot cultures were preserved at 4°C in the dark in a Murashige and Skoog basal medium containing one of two different concentrations of sucrose (20 and 30 g/l) and with or without growth regulators. We tested the effects of cold storage, genotype and media on survival and re-growth capacity of explants after 3, 6, 9 and 12 months of storage. Effective minimal growth under cold conditions occurred in all four genotypes. The media composition did not affect survival, which, instead, appeared to be genotype-dependent. P. domestica genotypes survived cold storage the longest, for 12 and 9 months; instead, P. cerasifera ones remained viable for up to 6 months. All genotypes retained proliferation capacity under standard conditions and their re-growth capacity seems to be strongly genotype-dependent, closely related to their individual performance in response to the experimental condition of storage.

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Section: Resultsmentioning

confidence: 99%

In vitro storage of plum germplasm by slow growth

Giannì¹,

Sottile²

2015

Hortic. Sci.

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“…The presence of cytokinins is associated with the appearance of necrosis on explants during cold storage, but the lack of roots leads to a decrease in endogenous cytokinin biosynthesis and a consequent reduction in cell division (Bairu et al 2009). …”

Section: Discussionmentioning

confidence: 99%

In vitro slow growth storage of Senecio macrophyllus shoots

2015

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Advances in biotechnology, especially in the field of in vitro culture techniques, led to the development of procedures that can be used as an excellent tool in plant conservation. The present study describes in vitro conservation of Senecio macrophyllus by slow-growth storage. Various sugar treatments, concentration of abscisic acid (ABA), light intensity and type of containers were tested. Viability and proliferation rate of shoots were evaluated 4 weeks after regrowth. The results obtained showed that polycarbonate boxes were a better type of containers for storage of S. macrophyllus shoots than glass vessels. Light negatively affected culture viability, multiplication rate and rooting response. In the case of Senecio macrophyllus the addition of ABA to storage medium stimulated survival and higher proliferation of shoots during regrowth in optimal conditions in comparison to shoots stored on medium without ABA. Shoots of S. macrophyllus were effectively stored for 6 months. All rooted shoots survived adaptation to field conditions and were able to flower in the second year after acclimatization.

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“…After 2 weeks of culturing, necrotic apices were observed in shoots growing in semisolid medium provided with either diffused or forced ventilation. This physiological disorder is commonly seen in in vitro cultures affecting a wide range of herbaceous and woody plants (Bairu et al, 2009a) and it is caused by a complex set of factors including plant growth regulators (Bairu et al, 2009b), calcium and boron deficiency (Hepler, 2005;Martin et al, 2007), medium type and salt strength (Jain et al, 2009;Takura and Kanwara, 2011), gelling agent (Singha et al, 1990), aeration (Bairu et al, 2009a), and pH (Pasqua et al, 2002). Our results indicated that the use of liquid medium through a TI system could overcome the shoot tip necrosis of H. heptaphyllus and promote growth and rooting of shoots.…”

Section: Resultsmentioning

confidence: 99%

Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants

Duarte¹,

Sansberro²,

Luna³

2016

Afr. J. Biotechnol.

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Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with medicinal and insecticidal properties. Poor seed viability of this species limits the conventional propagation practice. In vitro germination of seeds was achieved in semi-solid Murashige and Skoog's (MS) medium. A disinfection protocol was optimized for germination and growth of seedlings in vitro. Proliferation of shoots from cotyledonary and stem nodal segments was achieved in semi-solid MS medium supplemented with 22.2 µM 6-benzyladenine. Temporary immersion culture promoted elongation and rooting of shoots and reduced the shoot tip necrosis compared with culture on semisolid medium (6.7±2.7 and 41.7±7.0%, respectively; P = 0.001). Plantlets derived from temporary immersion showed better performance during the acclimatization phase. This is the first report on micropropagation of H. heptaphyllus.

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Factors contributing to in vitro shoot-tip necrosis and their physiological interactions

Cited by 70 publications

References 67 publications

In vitro storage of plum germplasm by slow growth

In vitro storage of plum germplasm by slow growth

In vitro slow growth storage of Senecio macrophyllus shoots

Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants

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