Factors determining phage stability/activity: challenges in practical phage application

Jończyk‐Matysiak, Ewa; Łodej, Norbert; Kula, Dominika; Owczarek, Barbara; Orwat, Filip; Międzybrodzki, Ryszard; Neuberg, J.; Bagińska, Natalia; Weber‐Dąbrowska, Beata; Górski, Andrzej

doi:10.1080/14787210.2019.1646126

Cited by 120 publications

(66 citation statements)

References 213 publications

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“…Phages are usually not selected based on their resistance to external factors, but rather due to their virulence, selectivity, ease of manipulation or modification. The recent review by Jończyk-Matysiak et al [ 200 ] described factors determining phage stability, with emphasis on the effect of chemical substances, pH, freezing, heat, UV-light, and methods of preparation and formulation. This appears crucial, as for instance, in the case of the “ Phagoburn” project, the patients received a very low dosage of phages (10 2 pfu/mL daily).…”

Section: Discussionmentioning

confidence: 99%

“…Phage outbreaks may contribute to significant economic losses due to delays in processing and manufacturing, the poor quality of the product, material contamination, and eventually complete production loss [ 210 ]. Physical or chemical agents are used, but more versatile, safe, and user-friendly methods of the deactivation of bacteriophages are needed [ 200 ]. Nanotechnology gives a promise to provide a solution.…”

Section: Discussionmentioning

confidence: 99%

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Application of Bacteriophages in Nanotechnology

Paczesny

Bielec

2020

Nanomaterials

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Bacteriophages (phages for short) are viruses, which have bacteria as hosts. The single phage body virion, is a colloidal particle, often possessing a dipole moment. As such, phages were used as perfectly monodisperse systems to study various physicochemical phenomena (e.g., transport or sedimentation in complex fluids), or in the material science (e.g., as scaffolds). Nevertheless, phages also execute the life cycle to multiply and produce progeny virions. Upon completion of the life cycle of phages, the host cells are usually destroyed. Natural abilities to bind to and kill bacteria were a starting point for utilizing phages in phage therapies (i.e., medical treatments that use phages to fight bacterial infections) and for bacteria detection. Numerous applications of phages became possible thanks to phage display—a method connecting the phenotype and genotype, which allows for selecting specific peptides or proteins with affinity to a given target. Here, we review the application of bacteriophages in nanoscience, emphasizing bio-related applications, material science, soft matter research, and physical chemistry.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Application of Bacteriophages in Nanotechnology

Paczesny

Bielec

2020

Nanomaterials

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“…In fact, the only factor inhibiting phage activity was temperature [73]. The importance of temperature for phage lytic activity has been pointed out countless times by several authors over the years [74][75][76][77], along with in-depth molecular analyses of this occurrence. Leon-Velarde et al [78] revealed that Yersinia enterocolitica outer membrane protein OmpF, which acts as a receptor for phages, is subject to strong repression at 37 • C. Thus, the tested phage lysed its host when grown at 25 • C but not at 37 • C. Intriguingly, recent articles imply that temperature may define the outcome of phage-bacteria interactions through the determination of the phage life cycle [79,80].…”

Section: Water Parametersmentioning

confidence: 99%

Phages as a Cohesive Prophylactic and Therapeutic Approach in Aquaculture Systems

2020

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Facing antibiotic resistance has provoked a continuously growing focus on phage therapy. Although the greatest emphasis has always been placed on phage treatment in humans, behind phage application lies a complex approach that can be usefully adopted by the food industry, from hatcheries and croplands to ready-to-eat products. Such diverse businesses require an efficient method for combating highly pathogenic bacteria since antibiotic resistance concerns every aspect of human life. Despite the vast abundance of phages on Earth, the aquatic environment has been considered their most natural habitat. Water favors multidirectional Brownian motion and increases the possibility of contact between phage particles and their bacterial hosts. As the global production of aquatic organisms has rapidly grown over the past decades, phage treatment of bacterial infections seems to be an obvious and promising solution in this market sector. Pathogenic bacteria, such as Aeromonas and Vibrio, have already proved to be responsible for mass mortalities in aquatic systems, resulting in economic losses. The main objective of this work is to summarize, from a scientific and industry perspective, the recent data regarding phage application in the form of targeted probiotics and therapeutic agents in aquaculture niches.

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“…There are many factors that may interfere with phage activity (when phages are intended as therapeutics), such as physico-chemical conditions, host physiological conditions, preparation composition or phage inability to penetrate and achieve high concentration at the site of infection ( Jończyk-Matysiak et al, 2019 ). Phage efficiency may depend on its properties, structure and biology as well as therapeutic expectations, dose, manner of application, as well as modifications that can improve their activity and availability.…”

Section: Limitations Of Phage Use For Elimination Of American Foulbromentioning

confidence: 99%

“…Phage efficiency may depend on its properties, structure and biology as well as therapeutic expectations, dose, manner of application, as well as modifications that can improve their activity and availability. The individual stability of the phage at different pH conditions is also an important issue, and this feature should be checked for each phage, as they tend to have different sensitivities to various physico-chemical conditions ( Jończyk-Matysiak et al, 2019 ). These features should be taken into account and all phages contained in the preparation should be fully characterized.…”

Section: Limitations Of Phage Use For Elimination Of American Foulbromentioning

confidence: 99%

Phages in Therapy and Prophylaxis of American Foulbrood – Recent Implications From Practical Applications

et al. 2020

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American foulbrood is one of the most serious and yet unsolved problems of beekeeping around the world, because it causes a disease leading to the weakening of the vitality of honey bee populations and huge economic losses both in agriculture and horticulture. The etiological agent of this dangerous disease is an extremely pathogenic spore-forming bacterium, Paenibacillus larvae , which makes treatment very difficult. What is more, the use of antibiotics in the European Union is forbidden due to restrictions related to the prevention of the presence of antibiotic residues in honey, as well as the global problem of spreading antibiotic resistance in case of bacterial strains. The only available solution is burning of entire bee colonies, which results in large economic losses. Therefore, bacteriophages and their lytic enzymes can be a real effective alternative in the treatment and prevention of this Apis mellifera disease. In this review, we summarize phage characteristics that make them a potentially useful tool in the fight against American foulbrood. In addition, we gathered data regarding phage application that have been described so far, and attempted to show practical implications and possible limitations of their usage.

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Factors determining phage stability/activity: challenges in practical phage application

Cited by 120 publications

References 213 publications

Application of Bacteriophages in Nanotechnology

Application of Bacteriophages in Nanotechnology

Phages as a Cohesive Prophylactic and Therapeutic Approach in Aquaculture Systems

Phages in Therapy and Prophylaxis of American Foulbrood – Recent Implications From Practical Applications

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