Factors that Differentially Affect Daytime and Nighttime Sleep in Drosophila melanogaster

Ishimoto, Hiroshi; Lark, Arianna R.S.; Kitamoto, Toshihiro

doi:10.3389/fneur.2012.00024

Cited by 62 publications

(71 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, distinctly different levels of cocaine-induced activity observed for wild-type animals during the day (low induced activity) vs. night (high induced activity) suggests potential separability between day and night that is nevertheless dependent in part on DopR function and may support spatially distinct circuits (Lebestky et al 2009). Given the separability of day and night circadian oscillators (Grima et al 2004; Stoleru et al 2004) and the differential expression of arousal activities in the day and night (Ishimoto et al 2012; Parisky et al 2016), this data identifies a requirement for DopR in the mushroom body for daytime sleep and arousal regulation. Future immunohistochemical and double mutant analyses with DopR and previously identified day sleep modulators, such as sex peptide (Isaac et al 2010), Ecdysone receptor, and DTS-3 (Ishimoto et al 2012), may also be informative in better understanding the network of molecules involved in day sleep regulation.…”

Section: Resultsmentioning

confidence: 85%

“…Sleep in Drosophila can be divided into multiple behavioral dimensions for investigation of potentially separable aspects of sleep behavior, such as onset of sleep, duration, number of sleep bouts, and average duration of individual sleep bouts. Furthermore, both genetic and environmental factors, such as temperature and light, can differentially affect parameters of Drosophila sleep in the day vs. night period (Ishimoto et al 2012; Parisky et al 2016). …”

mentioning

confidence: 99%

See 1 more Smart Citation

A Genetic Screen To Assess Dopamine Receptor (DopR1) Dependent Sleep Regulation inDrosophila

Jiang

Pitmon

Berry

et al. 2016

G3 Genes|Genomes|Genetics

View full text Add to dashboard Cite

Sleep is an essential behavioral state of rest that is regulated by homeostatic drives to ensure a balance of sleep and activity, as well as independent arousal mechanisms in the central brain. Dopamine has been identified as a critical regulator of both sleep behavior and arousal. Here, we present results of a genetic screen that selectively restored the Dopamine Receptor (DopR/DopR1/dumb) to specific neuroanatomical regions of the adult Drosophila brain to assess requirements for DopR in sleep behavior. We have identified subsets of the mushroom body that utilizes DopR in daytime sleep regulation. These data are supported by multiple examples of spatially restricted genetic rescue data in discrete circuits of the mushroom body, as well as immunohistochemistry that corroborates the localization of DopR protein within mushroom body circuits. Independent loss of function data using an inducible RNAi construct in the same specific circuits also supports a requirement for DopR in daytime sleep. Additional circuit activation of discrete DopR+ mushroom body neurons also suggests roles for these subpopulations in sleep behavior. These conclusions support a new separable function for DopR in daytime sleep regulation within the mushroom body. This daytime regulation is independent of the known role of DopR in nighttime sleep, which is regulated within the Fan-Shaped Body (FSB). This study provides new neuroanatomical loci for exploration of dopaminergic sleep functions in Drosophila, and expands our understanding of sleep regulation during the day vs. night.

show abstract

Section: Resultsmentioning

confidence: 85%

mentioning

confidence: 99%

A Genetic Screen To Assess Dopamine Receptor (DopR1) Dependent Sleep Regulation inDrosophila

Jiang

Pitmon

Berry

et al. 2016

G3 Genes|Genomes|Genetics

View full text Add to dashboard Cite

show abstract

“…WT Drosophila tend to sleep more during the day and less at night at high temperatures (63), as shown in Fig. 5B.…”

Section: Resultsmentioning

confidence: 90%

Temperature compensation and temperature sensation in the circadian clock

Kidd

Young

Siggia

2015

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

All known circadian clocks have an endogenous period that is remarkably insensitive to temperature, a property known as temperature compensation, while at the same time being readily entrained by a diurnal temperature oscillation. Although temperature compensation and entrainment are defining features of circadian clocks, their mechanisms remain poorly understood. Most models presume that multiple steps in the circadian cycle are temperature-dependent, thus facilitating temperature entrainment, but then insist that the effect of changes around the cycle sums to zero to enforce temperature compensation. An alternative theory proposes that the circadian oscillator evolved from an adaptive temperature sensor: a gene circuit that responds only to temperature changes. This theory implies that temperature changes should linearly rescale the amplitudes of clock component oscillations but leave phase relationships and shapes unchanged. We show using timeless luciferase reporter measurements and Western blots against TIMELESS protein that this prediction is satisfied by the Drosophila circadian clock. We also review evidence for pathways that couple temperature to the circadian clock, and show previously unidentified evidence for coupling between the Drosophila clock and the heat-shock pathway.circadian clock | temperature compensation | mathematical models C ircadian rhythms are daily oscillations in gene expression and protein concentration that regulate sleep (1, 2), metabolism (3,4), and a host of other biological processes (5-8). Circadian oscillations are known to be present in nearly all animals and plants, as well as some fungi and bacteria (9). In all organisms in which circadian oscillations have been observed, circadian oscillations satisfy three defining properties (10). First, circadian oscillations are self-sustained and spontaneously maintain a period of about 24 h. Second, the circadian rhythm is sensitive to light and temperature and can be synchronized to external oscillations in the quantity of either. Third, the period of the circadian oscillation is "temperature-compensated"; that is, the endogenous period of the oscillation is relatively insensitive to temperature.The genetic basis of circadian clocks has been elucidated in considerable detail over the past two decades, particularly in the fruitfly Drosophila melanogaster, the mouse Mus musculus, and the bread mold Neurospora crassa (11). In Drosophila, a self-sustained circadian oscillation in neurons is generated when a pair of proteins, PERIOD (PER) and TIMELESS (TIM), dimerize and, after some delay, translocate into the nucleus, where the proteins repress their own transcription by inactivating a transcription factor dimer composed of the proteins CLOCK and CYCLE. Light sensitivity is mediated by the blue light photoreceptor CRYPTOCHROME (CRY), which upon activation binds TIMELESS and promotes TIMELESS degradation. A remarkably similar mechanism underlies circadian clocks in mouse and Neurospora. The circadian clock of cyanobacteria has also bee...

show abstract

“…In flies it has been shown to effect locomotor activity at mid-day, during siesta [16, 17] and sleep itself [18]. The level of splicing of the circadian clock gene period is associated with this modulation [19, 20].…”

Section: Introductionmentioning

confidence: 99%

Reorganization of Sleep by Temperature in Drosophila Requires Light, the Homeostat, and the Circadian Clock

et al. 2016

View full text Add to dashboard Cite

SUMMARY Increasing ambient temperature reorganizes the Drosophila sleep pattern in a way similar to the human response to heat, increasing daytime sleep while decreasing nighttime sleep. Mutation of core circadian genes blocks the immediate increase in daytime sleep, but not the heat-stimulated decrease in nighttime sleep, when animals are in a light:dark cycle. The ability of per01 flies to increase daytime sleep in light:dark can be rescued by expression of PER in either LNv or DN1p clock cells and does not require rescue of locomotor rhythms. Prolonged heat exposure engages the homeostat to maintain daytime sleep in the face of nighttime sleep loss. In constant darkness, all genotypes show an immediate decrease in sleep in response to temperature shift during the subjective day, implying that the absence of light input uncovers a clock-independent proarousal effect of increased temperature. Interestingly, the effects of temperature on nighttime sleep are blunted in constant darkness and in cryOUT mutants in light:dark, suggesting that they are dependent on the presence of light the previous day. In contrast, flies of all genotypes kept in constant light sleep more at all times of day in response to high temperature, indicating that the presence of light can invert the normal nighttime response to increased temperature. The effect of temperature on sleep thus reflects coordinated regulation by light, the homeostat, and components of the clock, allowing animals to reorganize sleep patterns in response to high temperature with rough preservation of the total amount of sleep.

show abstract

Factors that Differentially Affect Daytime and Nighttime Sleep in Drosophila melanogaster

Cited by 62 publications

References 30 publications

A Genetic Screen To Assess Dopamine Receptor (DopR1) Dependent Sleep Regulation inDrosophila

A Genetic Screen To Assess Dopamine Receptor (DopR1) Dependent Sleep Regulation inDrosophila

Temperature compensation and temperature sensation in the circadian clock

Reorganization of Sleep by Temperature in Drosophila Requires Light, the Homeostat, and the Circadian Clock

Contact Info

Product

Resources

About