Faeces as a source of microbial enzymes for estimating digestibility.

Omed, H. M.; Lovett, D. K.; Axford, R. F. E.; Givens, D. I.; Owen, E.

doi:10.1079/9780851993447.0135

Cited by 27 publications

(33 citation statements)

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“…Cutrignelli et al (2005) did not find any significant differences when incubating different feedstuffs with faecal and ruminal inoculum from buffalo. It seems evident that there is an interaction of inoculum x substrate, a likely consequence of the different composition of the microbial population present in rumen liquor and in the caecal digesta (Omed et al, 2000). Adaptation to sugars did not influence the amount of VFA produced but had a moderate though significant effect on VFA molar proportions, decreasing the proportions of acetate and butyrate and increasing the proportions of propionate.…”

Section: Discussionmentioning

confidence: 98%

In Vitro Rumen Fermentation Characteristics of Some Naturally Occurring and Synthetic Sugars

Ahmed

Minuti

Bani

2013

Italian Journal of Animal Science

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Soluble sugars are thought to play an important role in the fermentation processes of the rumen but their actual fermentation rate has not been fully assessed. Some sugars are also used as markers to assess gut permeability in monogastrics but their use in ruminants can be compromised by the hydrolytic activity of rumen microflora. This study aimed to evaluate the fermentability of some naturally occurring and synthetic soluble sugars. The synthetic soluble sugars were included to verify their possible use as markers for studies of gut permeability in ruminants. In vitro gas and volatile fatty acid (VFA) production from glucose, fructose, xylose, galactose, sucrose, lactose, arabinose mannitol, lactulose and sucralose were measured in a 24 h-incubation trial using ruminal fluid from heifers adapted or not-adapted to additional sugars in the diet, and with caecal content as inocula. Gas production from the same sugars was further evaluated in a 72 h-incubation trial with not-adapted rumen fluid only. Gas and VFA production were not affected by feeding additional sugars, but significant effects of inocula (ruminal vs caecal), sugars and their interaction were observed. Caecal inoculum produced less gas but higher VFA than ruminal inocula. Fructose and glucose had the highest rates of gas production (10.57% h -1 and 10.42% h -1 , respectively), and lactulose and mannitol the lowest (3.47% h -1 and 4.63% h -1 , respectively) when fermented with ruminal fluid. Sucralose seemed to have a negative effect on microbial fermentations. Our results indicate that lactulose and mannitol might largely escape rumen fermentation, suggesting their possible use as markers to test gut permeability also in ruminants. This needs to be verified in vivo.

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Section: Discussionmentioning

confidence: 98%

In Vitro Rumen Fermentation Characteristics of Some Naturally Occurring and Synthetic Sugars

Ahmed

Minuti

Bani

2013

Italian Journal of Animal Science

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“…Araştırıcılar [24] kaba yemlerin iki aşamalı İVOMS değerlerinin belirlenmesinde +18°C'de 48 saat muhafaza edilen rumen sıvısının kullanılabileceğini ancak iki aşamalı in vitro sindirim metodunun mikrobiyal sindirim aşamasındaki 48 saatlik sürenin yetersiz olduğunu bildirmişlerdir. Bazı çalışmalarda [24][25][26] , araştırmacılar kaba yemler için iki aşamalı in vitro sindirim metodunun mikrobiyal sindirim aşamasındaki 48 saatlik sürenin 72 saate çıkarıl-masının gerektiğini bildirilmektedir. Benzer şekilde Denek ve ark.…”

Section: Tartişma Ve Sonuçunclassified

An Investigation on Usage of Frozen Rumen Fluid with Adding Dimethyl Sulfoxide and Different Freezing Methods for Determination of in Vitro Digestibility

Denek¹,

Can²,

Avcı³

2016

Kafkas Univ Vet Fak Derg

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Bu çalışmada, rumen sıvısı kaynağı olarak; taze rumen sıvısı, sıvı azot içerisinde dimetilsülfoksit (DMSO) katkısız ve katkılı (%5), derin dondurucuda DMSO katkısız ve katkılı (%5) dondurulmuş rumen sıvısı kullanılmıştır. Çalışmada 4 farklı kaba yem (mısır silajı, yonca kuru otu, mercimek samanı, buğday samanı), 4 farklı karma yem (sığır besi yemi, sığır süt yemi, toklu besi yemi ve buzağı büyütme yemi) ve 4 farklı yem hammaddesi (arpa, mısır, pamuk tohumu küspesi, soya küspesi) yem materyali olarak kullanılmıştır. İki aşamalı sindirim yönteminde deneme yemleri genel olarak değerlendirildiğinde gerek sıvı azot içerisinde ve gerekse derin dondurucuda dondurulan rumen sıvılarından elde edilen in vitro organik madde sindirim (İVOMS) değerleri taze rumen sıvısından elde edilmiş değerlerden düşük (P<0.05) bulunmuştur. Gaz üretim yönteminde ise yemlerin tamamı için %5 DMSO katkısı ile sıvı azot içerisinde dondurulmuş rumen sıvısından elde edilen İVOMS değerleri taze rumen sıvısından elde edilen değerler ile benzer (P>0.05) bulunmuştur. Araştırmada değerlendirilen tüm dondurulmuş rumen sıvılarındaki toplam anaerob bakteri ve toplam protozoa sayılarının, taze rumen sıvısındaki toplam anaerob bakteri ve protozoa sayılarına kıyasla azaldığı (P<0.05) görülmüştür. Protozoa türleri incelendiğinde %5 DMSO ilave edilerek sıvı azot içerisinde dondurulmuş rumen sıvısındaki canlı protozoa sayılarının kontrol grubu ile genel olarak benzer (P>0.05) olduğu belirlenmiştir. Bu araştırmadan elde edilen sonuçlara göre, %5 DMSO katkısı ile sıvı azot tankı içerisinde dondurulmuş rumen sıvılarının in vitro gaz üretim tekniğinde kullanılabileceği sonucuna varılmıştır. Anahtar sözcükler: Rumen sıvısı, in vitro sindirim, Dondurma, Sıvı azot, Derin dondurucu, Dimetilsülfoksit, DMSO An Investigation on Usage of Frozen Rumen Fluid with Adding Dimethylsulfoxide and Different Freezing Methods for Determination of in Vitro Digestibility AbstractIn this study, fresh rumen fluid, liquid nitrogen frozen rumen fluids with addition of 5% dimethylsulfoxide (DMSO) or without DMSO, deep freeze frozen rumen fluid addition of 5% DMSO or without DMSO were investigated as inoculum sources. Four roughages (corn silage, alfalfa hay, lentil straw, wheat straw), four commercial feeds (beef finishing, dairy cattle, ram lamb finishing, calf growing feeds) and four feed ingredients (barley, corn, cotton seed meal, soybean meal) were chosen as feed materials. Values of in vitro organic matter digestibility (IVOMD) of feeds using fresh rumen fluid were found generally higher than the values obtained from nitrogen or deep freeze frozen inoculum sources (P<0.05) in two stage in vitro procedure. Values of IVOMD via gas production technique were similar (P>0.05) with using liquid nitrogen frozen rumen fluids with addition of 5% DMSO. Number of the anaerobic bacteria and total protozoa were diminished with both freezing methods comparison with fresh rumen fluid (P<0.05). Species of protozoa count of fresh rumen fluid and nitrogen frozen with 5% DMSO were found generally sim...

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“…Sin embargo, se ha observado que en la experimentación con heces como inócu-lo son mayores los tiempos de colonización y es menor la capacidad de fermentación, que posiblemente se deba a una menor actividad microbiana en razón a que los microorganismos son originados principalmente en el ciego y el colon y no en el rumen como en la téc-nica tradicional. La explicación a lo anterior se soporta en que la cantidad de bacterias celulolíticas y otros tipos de microorganismos es mayor en el rumen que en el intestino grueso o en las heces [53,54]; los inó-culos fecales pueden contener algunos hongos anaerobios pero muy poca población de protozoarios [42], y, por último, de alguna manera los microorganismos allí contenidos pueden presentar una menor actividad metabólica en contraste con los situados en el de licor ruminal [55]. Así pues, se logra evidenciar la reducida fermentación en el ciego y el colon con respecto a la que ocurre en el rumen.…”

Section: Limitaciones De Los Métodos De Digestibilidad In Vitro De Prunclassified

Estimación de la técnica in vitro de gases frente a otras técnicas de digestibilidad

et al. 2013

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Cómo citar este artículo: Vargas-Bayona J, Mejía-Porras G, Bedoya-Mashuth J, Gómez-Patiño JF. Estimación de la técnica in vitro de gases frente a otras técnicas de digestibilidad. Spei Domus. 2013; 9(18): 59-70. Resumen. Para estimar el metabolismo de los alimentos consumidos por rumiantes, se han creado diversas técnicas de análisis nutricional que aportan información valiosa a la investigación en nutrición animal, buscando, entre otros, criterios para aumentar la eficiencia productiva en los sistemas tropicales de producción, considerando que los forrajes del trópico son la base de alimentación en rumiantes. De ahí la importancia de revisar y documentar sobre las diferentes técnicas de digestibilidad, para orientar hacia la mejor elección, a partir de referentes nacionales e internacionales. El objetivo de este reporte fue realizar una revisión de literatura sobre las técnicas de digestibilidad de forrajes utilizadas para estimar el comportamiento general de los alimentos para rumiantes. Para este fueron revisadas y analizadas más de 50 publicaciones, que arrojaron información sobre técnicas de digestibilidad in vivo, in situ e in vitro, y los alcances y limitaciones de estas. Como resultado se puede inferir que las técnicas se han convertido en una herramienta de gran importancia en el bioensayo, dada la facilidad para desarrollarla, costos reducidos, aplicación en el ámbito local, información valiosa que aporta, teniendo en cuenta que son escasos los estudios sobre forrajes tropicales. A partir de los hallazgos encontrados sobre las técnicas de digestibilidad de forrajes, se puede concluir que la técnica de digestibilidad in vitro de producción de gases es la herramienta más versátil; presenta procedimientos didácticos y accesibles; acarrea menos costos; además representa una alternativa más compatible para las legislaciones de bienestar animal, al usar las heces como inóculo. Sin embargo, es necesario realizar más estudios sobre los inóculos utilizados, debido a que los protocolos no están estandarizados y a que el comportamiento cinético en los ensayos realizados no ha arrojado los resultados esperados.Palabras clave: bioensayo, inóculo, licor ruminal, rumiantes, transductor de presión. Comparison of the In vitro Gas Technique to Other Digestibility TechniquesAbstract. To estimate the metabolism of the food consumed by ruminants, various nutritional analysis techniques have been developed to provide valuable information for research into animal nutrition. Objectives include criteria to increase productive efficiency in tropical production systems, particularly when tropical forages are the nutritional basis for ruminants. Thus, the motivation to review and document different digestibility techniques to determine the best choice among national and international references. The aim was to review the literature about forage digestibility techniques developed for estimating the overall behavior of foods in ruminants. More than 50 references were reviewed, obtaining information on in vivo, in situ and in vit...

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Faeces as a source of microbial enzymes for estimating digestibility.

Cited by 27 publications

References 0 publications

In Vitro Rumen Fermentation Characteristics of Some Naturally Occurring and Synthetic Sugars

In Vitro Rumen Fermentation Characteristics of Some Naturally Occurring and Synthetic Sugars

An Investigation on Usage of Frozen Rumen Fluid with Adding Dimethyl Sulfoxide and Different Freezing Methods for Determination of in Vitro Digestibility

Estimación de la técnica in vitro de gases frente a otras técnicas de digestibilidad

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