Faint object camera: European contribution to the Hubble Space Telescope

Kröger, Heinz; Schmidt, Gundolf; Pailer, N.

doi:10.1016/0094-5765(92)90063-o

Cited by 18 publications

(14 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…43,44 The Hubble Space Telescope's Faint Object Camera, and, more recently, the European Space Agency's X-ray Multi-Mirror satellite, launched in 1999, were fitted with a photon counting imaging optical monitor. 45,46 Linearity, high dynamic range, high sensitivity, zero read-out noise, large area, well-defined Poisson statistics and good spectral response in the UV are particular strengths of the photon counting imaging approach. Applications of photon counting imaging to diverse fields such as autoradiography, 47 bioluminescence 48 and fluorescence imaging 49 have also been reported.…”

Section: Photon Counting Imagingmentioning

confidence: 99%

Time-resolved fluorescence microscopy

et al. 2007

View full text Add to dashboard Cite

Fluorescence imaging techniques are powerful tools in the biological and biomedical sciences, because they are minimally invasive and can be applied to live cells and tissues. The fluorescence emission can be characterized not only by its intensity and position, by also by its fluorescence lifetime, polarization and wavelength. Fluorescence Lifetime Imaging (FLIM) in particular has emerged as a key technique to image the environment and interaction of specific proteins in living cells. Using a time-correlated single photon counting (TCSPC)-based FLIM set-up, we find that the fluorescence lifetime of GFP-tagged proteins in cells is a function of the refractive index of the medium the cells are suspended in. In addition, combining Fluorescence Recovery After Photobleaching (FRAP) of fluorescently labeled proteins of different sizes in sol gels with time-resolved fluorescence anisotropy measurements, we demonstrate that we can measure their lateral and rotational diffusion. This allows us to infer the size and connectivity of the pores in the sol gel matrix. Moreover, wide-field photon counting imaging, originally developed for astronomical applications, is a powerful imaging method because of its high sensitivity and excellent signal-to-noise ratio. It has a distinct advantage over CCD-based imaging due to the ability to time the arrival of individual photons. The potential of time-resolved widefield photon counting imaging with a fast CMOS camera applied to luminescence microscopy is demonstrated.

show abstract

Section: Photon Counting Imagingmentioning

confidence: 99%

Time-resolved fluorescence microscopy

et al. 2007

View full text Add to dashboard Cite

show abstract

“…There are various technological implementations of photon counting image intensifiers, e.g. a cascade intensifier [28] was used on the Hubble space telescope's faint object camera [29,30], but the most modern implementations of this technique employ microchannel plates (MCPs), as discussed below in section 4.1.…”

Section: Wide-field Tcspcmentioning

confidence: 99%

Wide-field TCSPC: methods and applications

Hirvonen

Suhling

2016

Meas. Sci. Technol.

View full text Add to dashboard Cite

“…Traditionally, image intensifiers have been used for wide-field photon counting imaging, for example the Faint Object Camera on the Hubble Space Telescope was based on a cascade intensifier. 10 The prime consideration at the time it was designed and built was sensitivity, not photon arrival timing. In modern microchannel plate (MCP)-based intensifiers, an incoming photon hits a photocathode creating a photoelectron, which creates secondary electrons in the MCP pores when it is accelerated through a high voltage.…”

Section: Techniquesmentioning

confidence: 99%

Wide-field TCSPC-based fluorescence lifetime imaging (FLIM) microscopy

et al. 2016

View full text Add to dashboard Cite

Time-correlated single photon counting (TCSPC) is a widely used, sensitive, precise, robust and mature technique to measure photon arrival times in applications such as fluorescence spectroscopy and microscopy, light detection and ranging (lidar) and optical tomography. Wide-field TCSPC detection techniques, where the position and the arrival time of the photons are recorded simultaneously, have seen several advances in the last few years, from the microsecond to the picosecond time scale. Here, we summarise some of our recent work in this field with emphasis on microsecond resolution phosphorescence lifetime imaging (PLIM) and nanosecond fluorescence lifetime imaging (FLIM) microscopy.

show abstract

Faint object camera: European contribution to the Hubble Space Telescope

Cited by 18 publications

References 0 publications

Time-resolved fluorescence microscopy

Time-resolved fluorescence microscopy

Wide-field TCSPC: methods and applications

Wide-field TCSPC-based fluorescence lifetime imaging (FLIM) microscopy

Contact Info

Product

Resources

About