Abstract:Background
The aging small intestine has a great impact on body health. One of the obvious characteristics is the degeneration of epithelium turnover. The acknowledged Lgr5+ intestinal stem cell is the key factor.
Methods
We used Lgr5-EGFP transgenic mice in three different ages (young group:3-6 months, middle group:12-14 months and old group:22-24 months) to mark the Lgr5+ISCs. We collected the jejunum for histology, immunofluorescence, western-blotting and PCR, we isolated the crypts for organoid culture a… Show more
Set email alert for when this publication receives citations?
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.