False Positive Reactions in Treponemal Tests

Julian, Alfredo J.; Portnoy, Joseph; Bossak, Hilfred N.

doi:10.1136/sti.39.1.30

Cited by 5 publications

(5 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The suggestion that rabbit tissues contain antigens cross-reacting with T . pallidurn is in agreement with the findings by Julian et al (1963) andMiller et al (1966) who observed that goats, upon prolonged immunization with rabbit testicular material, developed T . pallidurn immobilizing antibodies.…”

Section: Discussionsupporting

confidence: 93%

STUDIES ON THE TREPONEMA PALLIDUM IMMOBILIZING ACTIVITY IN NORMAL HUMAN SERUM

Hederstedt¹

1976

Acta Pathologica Microbiologica Scandinavica Section C Immunolo

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 93%

STUDIES ON THE TREPONEMA PALLIDUM IMMOBILIZING ACTIVITY IN NORMAL HUMAN SERUM

Hederstedt¹

1976

Acta Pathologica Microbiologica Scandinavica Section C Immunolo

View full text Add to dashboard Cite

show abstract

“…Because the T. pallidum routinely used as antigen in the present and other immunofluorescence tests had been harvested from rabbit testicular syphilomas, substances of rabbit origin may have coated or been absorbed onto the organisms (10,12). Thus, it is conceivable that some of the natural reactivities we detected were directed at rabbit antigens in close association with the treponemes and not at the organisms themselves.…”

Section: Downloaded Frommentioning

confidence: 99%

Immunoglobulin Class of Natural Human Antibodies Reactive with Treponema pallidum

Julian¹,

Logan²,

Norins³

et al. 1970

Infect Immun

Self Cite

View full text Add to dashboard Cite

To aid the development of serological tests for incubating syphilis, a characterization was made of the background of natural anti-Treponema pallidum antibodies against which the initial immune response to syphilis takes place. Sera from presumed normal persons were studied with monospecific antisera in an indirect fluorescent-antibody procedure. Of 36 sera tested at a 1:5 dilution, all showed IgG reactivity with T. pallidum (Nichols strain), 58% showed IgM reactivity, and 20% showed IgA reactivity. The titer of IgG reactivity was considerably higher than that of the other two immunoglobulins. Heating the sera for 1 hr at 65 C abolished IgA and IgM anti-T. pallidum reactivity, but one-third of the sera retained IgG reactivity. Human Cohn fractions II and III, from three commercial sources contained mostly IgG antibodies reactive with T. pallidum, but IgM and IgA antibodies were also present. IgG reactivity was found in a pool of presumably normal maternal sera from 20 mothers and in a corresponding pool of umbilical cord serum from their infants. IgM and IgA reactivities were present only in the maternal serum pool.

show abstract

“…Makleit, Rethy, and Richter (1964/5) examined such antigens by thin layer and gas chromatography and found many which were not suited for demonstrating syphilitic reagins. Julian, Portnoy, and Bossak (1963) succeeded in producing false positive TPI tests in goats injected with homogenates of normal rabbit testes. They do not deny that this may also happen in testing human sera, since it must be considered that traces of material from rabbit testes always adhere to the treponemata prepared for the TPI test.…”

Section: Resultsmentioning

confidence: 99%

Treponemal immobilization tests in leprosy.

Ruge¹

1967

Sexually Transmitted Infections

View full text Add to dashboard Cite

False Positive Reactions in Treponemal Tests

Cited by 5 publications

References 5 publications

STUDIES ON THE TREPONEMA PALLIDUM IMMOBILIZING ACTIVITY IN NORMAL HUMAN SERUM

STUDIES ON THE TREPONEMA PALLIDUM IMMOBILIZING ACTIVITY IN NORMAL HUMAN SERUM

Immunoglobulin Class of Natural Human Antibodies Reactive with Treponema pallidum

Treponemal immobilization tests in leprosy.

Contact Info

Product

Resources

About