2014
DOI: 10.1186/s13568-014-0036-9
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Family 1 carbohydrate binding-modules enhance saccharification rates

Abstract: Cellulose degrading enzymes usually have a two-domain structure consisting of a catalytic domain and a non-catalytic carbohydrate-binding module. Although it is well known the importance of those modules in cell wall degrading process, their function is not yet fully understood. Here, we analyze the cellulose-hydrolysis activity enhancement promoted by the cellobiohydrolase I carbohydrate-binding module from Trichoderma harzianum. It was cloned, expressed, purified and used in combination with either a commerc… Show more

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Cited by 30 publications
(17 citation statements)
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“…Biomass degradation is necessary as a pretreatment process for the saccharification of lignocellulolytic material, which would aid bioethanol or biofuel production. In addition to their use as components of chimeric enzymes, CBMs have been used as additives in reactions during the saccharification process of crystalline cellulose, increasing the production of glucose up to 30% …”
Section: Applications Of Cbms In Biotechnologymentioning
confidence: 99%
See 1 more Smart Citation
“…Biomass degradation is necessary as a pretreatment process for the saccharification of lignocellulolytic material, which would aid bioethanol or biofuel production. In addition to their use as components of chimeric enzymes, CBMs have been used as additives in reactions during the saccharification process of crystalline cellulose, increasing the production of glucose up to 30% …”
Section: Applications Of Cbms In Biotechnologymentioning
confidence: 99%
“…In addition to their use as components of chimeric enzymes, CBMs have been used as additives in reactions during the saccharification process of crystalline cellulose, increasing the production of glucose up to 30%. 79 Lignocellulolytic material is not easily digested into sugars mainly due to the presence of lignin. Low-lignin rice was developed through the expression of a laccasecellulose-binding domain (CBD) fusion protein, and this protein increases saccharification efficiency (up to 1.5fold) and modifications in the cell wall composition.…”
Section: Applications Of Cbms In Biotechnologymentioning
confidence: 99%
“…Although white rot fungi have been associated with lack of CBMs, G. lucidum and G. boninense contained 10 and 12 out of 16 total families of CBMs, respectively. It is notable that G. boninense possessed high copies of CBM1, an important fungal CBM that uses cellulose and chitin as substrates for polysaccharide-degrading activities (Mello & Polikarpov, 2014;Várnai et al, 2014). Both fungi shared similar GTs except for GT65 and GT41 that were found only in G.…”
Section: Results and Discussion Characterization Of Carbohydrate-activementioning
confidence: 99%
“…In contrast, Cip2, PcGE1 from P. chrysoporium (Ďuranová et al, 2009b), and CuGE from C. unicolor (d 'Errico et al, 2015) contain type 1 CBM at the N-terminus of the catalytic domain. CBMs are classified into subfamilies based on the amino acid sequence similarity and most of the fungal CBMs in cellulases belong to the family 1, in which three aromatic residues create a planar structure to bind to the crystallized surfaces of chitin/cellulose (Mello and Polikarpov, 2014). When expressed in P. pastoris, a protein of 44 kDa which is nearly equal to the calculated size of myc-and hexahistidine-tagged NcGE was produced.…”
Section: Discussionmentioning
confidence: 99%