FaPEx® Multipesticide Residues Extraction Kit for Minimizing Sample Preparation Time in Agricultural Produce

Abstract

Background: The quick, easy, cheap, effective, rugged, and safe method, generally applied to the determination of pesticide residues in food, has been recently modified and adopted for the analysis of pesticide residues worldwide, including Taiwan. Objective: The method still needs to be improved, particularly in efficiency and normalization, because the time costs 20–30 min for extraction and cleanup of multiple pesticides in every food. Methods: In this study, we present a highly efficient and simple sample preparation method that was developed for the determination of 380 pesticide residues in food, including mangoes, scallions, bok choy, apples, carrots, and pea seedlings. The homogenized 1 g samples mixed with 5 mL 1.0% acetic acid in acetonitrile were pushed through the FaPEx® (fast pesticide extraction) kits in a dropwise manner to obtain sample extracts. The total processing time was less than 15 min. The extracts were subject to chromatographic separation followed by GC–tandem MS (MS/MS) and LC–MS/MS analysis. Results: For more than 270 pesticide residues in these foods, the test gave acceptable mean recoveries, ranging between 70 and 120%, and relative SDs below 20%. The LOQ was at least 0.01 mg/kg for 380 pesticides. Conclusions: The developed method can greatly reduce the time needed for multiple pesticide residues analysis. Highlights: FaPEx is used for the extraction of pesticide residues that relies on single-use pre-filled sealed cartridges. FaPEx diminishes operation time, glassware demand, and laboratory space requirement. It also significantly decreases the amount of chemical solvents.

Cumulative Dietary Risk Assessment of Benzophenone-Type Photoinitiators from Packaged Foodstuffs

“…To extract BPs from foods, the fast pesticide extraction (FaPEx) method, a novel and rapid technique for extraction of pesticide residues in agricultural products, was used [ 18 ]. FaPEx is currently the fastest method available worldwide.…”

“…Fast pesticide extraction (FaPEx) is a method used to extract pesticide residues in agricultural samples by using single-use prefilled sealed cartridges; this method is innovative, simple, and fast and is a simplified version of the QuEChERS method that is based on the same principles [ 18 ]. We have developed a FaPEx technique coupled with ultra-HPLC (UHPLC)-MS/MS to simultaneously analyze the levels of targeted BPs in the present study.…”

Rapid determination of benzophenone derivatives in cereals using FaPEx coupled with ultra–high-performance liquid chromatography–tandem mass spectrometry

“…The FaPEx technique was developed by the Taiwan Agricultural Chemicals and Toxic Substances Research Institute and authorized by Uni-Onward. The major components involved in FaPEx are MgSO 4 , PSA, C18, and graphitized carbon black [ 20 ]. FaPEx is an easy technique, and it has a short extraction time (<5 min) and produces remarkably low amounts of waste solvents and chemicals.…”

“…However, some methods are limited in terms of instrument sensitivity or the number of BPs that can be simultaneously analyzed. Fast pesticide extraction (FaPEx) is a method used to extract pesticide residues in agricultural samples by using single-use prefilled sealed cartridges; this method is innovative, simple, and fast and is a simplified version of the QuEChERS method that is based on the same principles [ 20 ].…”

Identification of Benzophenone Analogs in Rice Cereal through Fast Pesticide Extraction and Ultrahigh-Performance Liquid Chromatography–Tandem Mass Spectrometry

“…Fast pesticide extraction (FaPEx) is a simplified version of the QuEChERS method and is based on the same principles. It involves using single-use prefilled sealed cartridges [ 24 ]. FaPEx is simple and fast and can be used to extract pesticide residues in agricultural samples.…”