Far infrared-assisted extraction followed by capillary electrophoresis for the determination of bioactive constituents in the leaves of Lycium barbarum Linn.

Duan, Haotian; Chen, Yi; Chen, Gang

doi:10.1016/j.chroma.2010.04.069

Cited by 56 publications

(41 citation statements)

References 22 publications

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“…As the amount of betaine in lycium fruit, is about 1% (Zhu, 1998), to obtain a significant amount, a large dose of lycium fruit would need to be consumed (e.g., 20-30 g). A method based on capillary electrophoresis with amperometric detection and far infrared-assisted extraction has determined rutin, gentisic acid, and quercetin in the leaves of L. barbarum (Duan, Chen, & Chen, 2010). A high-performance liquid chromatography-diode array detection-mass spectrometry method with electrospray ionization mode (HPLC-DAD-ESI-MS) was recently developed for simultaneous determination of phenolic acids and flavonoids in fruits of L. barbarum.…”

Section: Other Compoundsmentioning

confidence: 99%

A review of botanical characteristics, phytochemistry, clinical relevance in efficacy and safety of Lycium barbarum fruit (Goji)

Amagase¹,

Farnsworth

2011

Food Research International

482

322

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Section: Other Compoundsmentioning

confidence: 99%

A review of botanical characteristics, phytochemistry, clinical relevance in efficacy and safety of Lycium barbarum fruit (Goji)

Amagase¹,

Farnsworth

2011

Food Research International

482

322

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“…Two detection techniques have been used to accurately determine the concentration of polyphenols in fruits of L. barbarum L.; these techniques include mass spectrometry (MS) and UV (Dong et al 2009;Inbaraj et al 2010;Le et al 2007;Qian et al 2004;Wu et al 2012). The separation of three flavonoids was also obtained with capillary electrophoresis with amperometric detection (Duan et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Chromatographic Determination of Phenolic Acids and Flavonoids in Lycium barbarum L. and Evaluation of Antioxidant Activity

Magiera

Zareba

2015

Food Anal. Methods

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Polyphenols are the important active compounds present in the fruits of Lycium barbarum L. The objectives of this study were to develop an ultra-high performance liquid chromatography with UV detection (UHPLC-UV) method for qualitative and quantitative analyses of phenolic acids and flavonoids in fruits of L. barbarum L. The isolation and enrichment of polyphenols from fruits were carried out by ultrasound-assisted extraction (UAE) in conjunction with solid-phase extraction (SPE). The analytes were separated on a Poroshell 120 EC-C18 column using a binary mobile phase composed of aqueous 0.05 % trifluoroacetic acid and acetonitrile in the gradient elution mode. Under these conditions, phenolic acids and flavonoids were separated in 11 min. The selectivity of the developed UHPLC-UV method was confirmed by comparison with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis. The validation parameters such as linearity, sensitivity, precision, and accuracy were found to be highly satisfactory. The optimized method was successfully applied to the analysis of polyphenols in fruits of L. barbarum L. Additionally, their free radicalscavenging activity was assessed by 1,1-diphenyl-2-picrylhydrazyl radical (DPPH • ).

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“…The CE−AD system used in this work has been described in our previous reports . A ±30 kV high‐voltage dc power supply (Shanghai Institute of Nuclear Research, China) provided a separation voltage between the two ends of the capillary.…”

Section: Methodsmentioning

confidence: 99%

Graphene/poly(ethylene‐co‐vinyl acetate) composite electrode fabricated by melt compounding for capillary electrophoretic determination of flavones in Cacumen platycladi

Sheng

Liu

Zhang

et al. 2013

J of Separation Science

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In this report, a graphene/poly(ethylene-co-vinyl acetate) composite electrode was fabricated by melt compounding for the amperometric detection of capillary electrophoresis. The composite electrode was fabricated by packing a mixture of graphene and melted poly(ethylene-co-vinyl acetate) in a piece of fused silica capillary under heat. The structure of the composite was investigated by scanning electron microscopy and Fourier transform infrared spectroscopy. The results indicated that graphene sheets were well dispersed in the composite to form an interconnected conducting network. The performance of this unique graphene-based detector has been demonstrated by separating and detecting rutin, quercitrin, kaempferol, and quercetin in Cacumen platycladi in combination with capillary electrophoresis. The four flavones have been well separated within 9 min in a 50-cm-long capillary at a separation voltage of 12 kV using a 50 mM sodium borate buffer (pH 9.2). The graphene-based detector offered significantly lower operating potentials, substantially enhanced signal-to-noise characteristics, lower expense of operation, high resistance to surface fouling, and enhanced stability. It showed long-term stability and repeatability with relative standard deviations of <5% for the peak current (n = 15).

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Far infrared-assisted extraction followed by capillary electrophoresis for the determination of bioactive constituents in the leaves of Lycium barbarum Linn.

Cited by 56 publications

References 22 publications

A review of botanical characteristics, phytochemistry, clinical relevance in efficacy and safety of Lycium barbarum fruit (Goji)

A review of botanical characteristics, phytochemistry, clinical relevance in efficacy and safety of Lycium barbarum fruit (Goji)

Chromatographic Determination of Phenolic Acids and Flavonoids in Lycium barbarum L. and Evaluation of Antioxidant Activity

Graphene/poly(ethylene‐co‐vinyl acetate) composite electrode fabricated by melt compounding for capillary electrophoretic determination of flavones in Cacumen platycladi

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