Färbung der Linsenkapsel mit Trypanblau zur Visualisierung der Kapsulorhexis bei Maturkataraktchirurgie

Melles, Gerrit R.J.; Waard, P W de; Pameijer, J. H.; Beekhuis, W. H.

doi:10.1055/s-2008-1034729

Cited by 18 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Trypan blue is commonly used for anterior capsule staining and its efficacy and toxicity have been well studied [4, 5, 6]. In the literature, methods have already been described for mixing trypan blue with viscoelastic agents for various purposes, for example, to achieve a limitation of color contact to the region of interest [7].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a Novel Blue-Colored Ophthalmic Viscoelastic Device Applied during Phacoemulsification in Eyes with Pseudoexfoliation Syndrome

Borkenstein¹,

Borkenstein²

2019

Case Rep Ophthalmol

View full text Add to dashboard Cite

Recently, a new development in the field of ophthalmic viscoelastic devices (OVDs) has been introduced. The blue-colored OVD Pe-Ha-Blue® PLUS combines the viscoelastic properties of OVD together with trypan blue for better visualization during surgery. The objective of this case series is to investigate whether the use of a blue-colored OVD during cataract surgery in patients with pseudoexfoliation syndrome (PXF) and narrow pupils (miosis) has advantages over the use of a clear standard OVD in combination with trypan blue. We included 52 eyes of 52 consecutive cataract patients diagnosed with PXF. Study participants were divided into two groups of 26 patients each, depending on which OVD was used during surgery (group A: blue-colored OVD; group B: standard clear OVD). Intraoperatively, we evaluated the operation time (surgery start to the beginning of phacoemulsification) and the surgeon’s satisfaction with the surgical workflow. Postoperative examinations were performed 6 h, 20 h, and 4 weeks after surgery and included corrected distance visual acuity (CDVA) and intraocular pressure (IOP). Our results show that there was a statistically significant time gain in group A in terms of surgery time. In addition, findings on surgeon satisfaction, postoperative CDVA and IOP suggest further benefits in group A. We discuss further properties and possible advantages which may result from the use of a blue-colored OVD. In summary, a blue-colored viscoelastic device can be a helpful alternative to clear OVD especially in challenging cases to improve the surgical workflow and make the whole procedure even safer and faster.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluation of a Novel Blue-Colored Ophthalmic Viscoelastic Device Applied during Phacoemulsification in Eyes with Pseudoexfoliation Syndrome

Borkenstein¹,

Borkenstein²

2019

Case Rep Ophthalmol

View full text Add to dashboard Cite

show abstract

“…TB is a widely used vital dye whose efficacy in terms of staining properties and cytotoxicity on various structures of the anterior segment of the eye has been investigated in several in vitro studies 2,3,11,12 and clinical trials 4,[13][14][15][16] .TB showed no cytotoxic effects in clinical studies, but in vitro studies proved that TB is toxic to cultured human corneal endothelial cells, 11 cultured rabbit corneal endothelial cells 2 or HeLa cells in a concentration-and/or time-dependent manner. 12 Basically, the use of TB requires a rapid rinsing of stained areas and a complete removal from the eye, as the critical concentration might be exceeded due to the weak miscibility in the eye.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Wash-out Properties after Use of the Vital Dye Trypan Blue in the Form of an Ophthalmic Dye and Bound in a Sodium Hyaluronate by Raman Spectroscopy

Borkenstein

Rattenberger

et al. 2021

Current Eye Research

View full text Add to dashboard Cite

“…Recently, TB has been used to stain and make the anterior capsule of the lens visible during mature cataract surgery [13, 14], and no toxicity to the anterior structures of the eye has been reported [15]. Follow-up examinations after 8 years have revealed no side effects of TB, as judged by the presence of corneal opacities, compared with nonstained aphakic and nonoperated control eyes.…”

Section: Discussionmentioning

confidence: 99%

Hypothermia Protects Cultured Human Retinal Pigment Epithelial Cells against Trypan Blue Toxicity

et al. 2006

View full text Add to dashboard Cite

Purpose: To determine whether trypan blue (TB) is toxic to cultured human retinal pigment epithelial (ARPE-19) cells, and whether hypothermia can protect ARPE-19 cells against TB toxicity. Methods: ARPE-19 cells were cultured and exposed to balanced salt solution as a control, while other cells were exposed to 0.05, 0.2, and 0.5% TB dye at 37 and 4°C for 5 and 30 min. The percentage of ARPE-19 cells that survived was determined by resazurin 1 day after the exposure. Results: A statistically significant decrease in the percentage of ARPE-19 cells surviving was found after exposure to 0.2 and 0.5% TB at any temperature or for any exposure duration (p < 0.01). The percentage of RPE cells surviving at 0.05% was not significantly different from that of controls except for a 30-min exposure at 37°C. The percentage of cells surviving at 4°C for a 5-min exposure to 0.5% TB and a 30-min exposure to 0.2 and 0.5% was significantly higher than that at 37°C under each condition (p < 0.01 for all). Conclusions: These results indicate that TB is toxic to human RPE cells, and the toxicity is dose- and exposure duration-dependent. Exposing the cells at 4°C had a protective effect against higher concentrations or longer exposure durations of TB compared to exposure at 37°C.

show abstract

Färbung der Linsenkapsel mit Trypanblau zur Visualisierung der Kapsulorhexis bei Maturkataraktchirurgie

Abstract: Trypan blue staining of the anterior lens capsule may therefore be a safe technique to facilitate the performance of a capsulorhexis in the absence of a red fundus reflex.

Cited by 18 publications

References 0 publications

Evaluation of a Novel Blue-Colored Ophthalmic Viscoelastic Device Applied during Phacoemulsification in Eyes with Pseudoexfoliation Syndrome

Evaluation of a Novel Blue-Colored Ophthalmic Viscoelastic Device Applied during Phacoemulsification in Eyes with Pseudoexfoliation Syndrome

Comparison of Wash-out Properties after Use of the Vital Dye Trypan Blue in the Form of an Ophthalmic Dye and Bound in a Sodium Hyaluronate by Raman Spectroscopy

Hypothermia Protects Cultured Human Retinal Pigment Epithelial Cells against Trypan Blue Toxicity

Contact Info

Product

Resources

About