Fast atom bombardment mass spectrometry of human proinsulin

Barber, M.; Bordoli, Robert S.; Elliott, Gerard J.; Horoch, Nicholas J.; Green, Brian N.

doi:10.1016/0006-291x(83)91025-2

Cited by 54 publications

(9 citation statements)

References 5 publications

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“…The phenylthiohydantoin amino acid derivatives were separated using an on-line Applied Biosystems phenylthiohydantoin analyser. The molecular mass of the peptide was measured with a fast-atom-bombardment mass spectrometer (JEOL JMS-HX 110) as described in [8].…”

Section: Methodsmentioning

confidence: 99%

Structure and biological activities of a new mastoparan isolated from the venom of the hornet Vespa basalis

Hwang

1991

Biochemical Journal

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By gel filtration on a Fractogel TSK HW 50 column followed by cation-exchange chromatography on CM-Trisacryl M, a tetradecapeptide amide, designated 'mastoparan B', was purified from the venom of the hornet Vespa basalis. Its amino acid sequence was determined as: Leu-Lys-Leu-Lys-Ser-Ile-Val-Ser-Trp-Ala-Lys-Lys-Val-Leu-NH2 and its molecular mass was measured to be 1611 Da by fast-atom-bombardment mass spectrometry. In addition to having a common structure of vespid mastoparans, the peptide shows a less hydrophobic sequence at positions 1, 2, 5, 8 and 9. The peptide caused liberation of histamine from rat peritoneal mast cells and induced oedema in the rat paw. However, the latter effect was inhibited by 'anti-serotonin' (anti-5-hydroxytryptamine) (cyproheptadine), but not by antihistamine (chlorpheniramine). The peptide also possesses a potent haemolytic activity which acts in synergy with the lethal protein of the venom, suggesting the possible involvement of mastoparan B in the lethal effect of Vespa basalis venom.

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Section: Methodsmentioning

confidence: 99%

Structure and biological activities of a new mastoparan isolated from the venom of the hornet Vespa basalis

Hwang

1991

Biochemical Journal

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“…This work showed that accelerated (fast) atoms, typically argon or xenon, could be used for ionisation of small biomolecules (i.e. masses below 10 kDa) combined with on-line fragmentation for structure determination 9 . This technique, termed fast atom bombardment (FAB), and the closely related method liquid matrix secondary ion MS (LSIMS), did not solve the problem of reaching higher masses but had a major impact on expectations on future success.…”

Section: Introductionmentioning

confidence: 99%

Mechanism of action of pyridazine analogues on protein tyrosine phosphatase 1B (PTP1B)

Tjernberg

Hallén

Schultz

et al. 2004

Bioorganic & Medicinal Chemistry Letters

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“…The RRK theory is the classical approximation of the Rice-Rampsberger-Kassel-Marcus (RRKM) theory. According to the latter, the rate constant k( E) at a given energy is T he use of mass spectrometry to characterize macromolecules is growing dramatically following the development of a variety of techniques [1][2][3][4] for generating large ions in the gas phase. However, structural information provided by mass spectrometry on large molecules is limited because the latter do not readily fragment [5,6].…”

mentioning

confidence: 99%

The effect of ion size on rate of dissociation: RRKM calculations on model large polypeptide ions

Griffin

McAdoo

1993

Journal of the American Society for Mass Spectrometry

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The larger an ion is, the less likely it is to decompose on mass spectrornetry time scales at given critical and internal energies. This is an obstacle to obtaining structural information on large molecules by mass spectrometry. We performed RRKM calculations on model ions with masses from 0.27 kDa to 102.4 kDa to explore what such calculations predict regarding this limitation. According to the calculations, it is impractical to add enough energy to fragment very large ions unless the decomposition has a low critical energy. It is suggested that ion-molecule reactions that are either very low in their critical energies or exothermic may be a feasible approach to fragmenting ionized macromolecules.

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Fast atom bombardment mass spectrometry of human proinsulin

Cited by 54 publications

References 5 publications

Structure and biological activities of a new mastoparan isolated from the venom of the hornet Vespa basalis

Structure and biological activities of a new mastoparan isolated from the venom of the hornet Vespa basalis

Mechanism of action of pyridazine analogues on protein tyrosine phosphatase 1B (PTP1B)

The effect of ion size on rate of dissociation: RRKM calculations on model large polypeptide ions

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