Fast molecular mass and size characterization of polysaccharides using asymmetrical flow field-flow fractionation-multiangle light scattering

Wittgren, Bengt; Wahlund, Karl-Gustav

doi:10.1016/s0021-9673(96)00777-7

Cited by 109 publications

(72 citation statements)

References 21 publications

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“…The conformation of dextran seems to be the more 'compact' one of the two, which is supported by the presence of branching points along the chain, whereas pullulan implies a random coil in a semi-good solvent. These a values are in good agreement with reported ones, being 0.44 and 0.38 for dextran, and 0.54 and 0.58 (Nordmeier, 1993 andWittgren andWahlund, 1997) for pullulan, correspondingly.…”

Section: Detergent±tag Interactionssupporting

confidence: 92%

“…An uncoated capillary exhibiting electroosmosis was used for this experiment to speed up analysis as the mobility is very low for the high-molecular-weight fractions. These biopolymers both comprise glucose but differ in the glycosidic bonds, pullulan being a linear polymer of a-(1 → 6) linked maltotriose blocks and usually envisaged as a random coil in aqueous solutions (Wittgren et al, 1997). Dextran consists of a-(1 → 6) linked glucose units but there are also branching points present along the chain and, the higher the degree of branching, the less a random coil behavior and the conformation can be visualized as sphere-like.…”

Section: Detergent±tag Interactionsmentioning

confidence: 99%

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Employment of detergent–tag/solute interactions in capillary electrophoresis of neutral polysaccharides

Tokarz

Gustavsson

Stefansson

2002

Biomedical Chromatography

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Neutral and inherently immobile polysaccharides are induced to migrate in an electric field through interactions with a detergent added to the electrophoretic electrolyte buffer. Before analysis the polysaccharides are converted to fluorescent derivatives to enable detection, but choice of a tag can also be utilized for modulation of the electrophoretic mobility. Three cases are discussed and exemplified, namely detergent-solute, detergent-solute+tag, and detergent-tag interactions. Anionic as well as cationic surfactants were exploited along with different derivatization reagents. Depending on the approach chosen, different kinds of information about sample composition and distribution(s) can be obtained, including degree of substitution, distribution of molecular weight (obtained in free solution without sieving media) and polymer conformation. A shift in polymer conformation upon a change in solvent composition can be monitored.

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Section: Detergent±tag Interactionssupporting

confidence: 92%

Section: Detergent±tag Interactionsmentioning

confidence: 99%

Employment of detergent–tag/solute interactions in capillary electrophoresis of neutral polysaccharides

Tokarz

Gustavsson

Stefansson

2002

Biomedical Chromatography

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“…By combining the Stokes-Einstein equation with the retention theory [169] Rh can be obtained from the elution time: Figure 12. Schematic illustration of the separation mechanism using asymmetrical flow field flow fractionation.…”

Section: Separation Principlementioning

confidence: 99%

“…By combining the Stokes-Einstein equation with the retention theory [169] R h can be obtained from the elution time:…”

Section: Separation Principlementioning

confidence: 99%

Size Separation Techniques for the Characterisation of Cross-Linked Casein: A Review of Methods and Their Applications

et al. 2018

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Abstract:Casein is the major protein fraction in milk, and its cross-linking has been a topic of scientific interest for many years. Enzymatic cross-linking has huge potential to modify relevant techno-functional properties of casein, whereas non-enzymatic cross-linking occurs naturally during the storage and processing of milk and dairy products. Two size separation techniques were applied for characterisation of these reactions: gel electrophoresis and size exclusion chromatography. This review summarises their separation principles and discusses the outcome of studies on cross-linked casein from the last~20 years. Both methods, however, show limitations concerning separation range and are applied mainly under denaturing and reducing conditions. In contrast, field flow fractionation has a broad separation range and can be easily applied under native conditions. Although this method has become a powerful tool in polymer and nanoparticle analysis and was used in few studies on casein micelles, it has not yet been applied to investigate cross-linked casein. Finally, the principles and requirements for absolute molar mass determination are reviewed, which will be of increased interest in the future since suitable calibration substances for casein polymers are scarce.

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“…Kammer and Förstner [9] including separation principles of FFF. A general overview about the applications of FFF on natural colloids is given in Beckett and Hart [10], an overview of the application of asymmetric FFF including laser light scattering detectors is given in [11]. For this study detectors with enhanced analytical capacities compared to [9] were employed.…”

Section: Fractionation and Analysismentioning

confidence: 99%

Colloidal Particles in Sediment Pore Waters: Particle Size Distributions and Associated Element Size Distribution in Anoxic and Re‐oxidized Samples, Obtained by FFF‐ICP‐MS Coupling

Kammer

Baborowski

Tadjiki

et al. 2003

Acta hydrochim. hydrobiol.

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Pore water samples from sediment cores of the rivers Saale and Elbe were examined for variations of colloidal heavy metal associations during transition from anoxic to oxic conditions. Asymmetric flow-field flow fractionation coupled to ICP-MS was used to collect size distributions and element-size distributions simultaneously. The data obtained reveal an increasing amount of colloidal bound main and trace elements in the pore waters during oxidation. Although there was a significant trend to increasing colloid concentrations and colloidal bound heavy metals over the observed 48 hour period of oxidation, differences between both samples were obvious. Values for molecular mass are approximated, since the spatial extension of macromolecules (humic substance) is depending on molecular structure and surrounding conditions. Operationally defined cut-offs are given for classical laboratory filtration (0.45 µm) and settling (2 µm).Größenbereiche natürlicher kolloidaler Partikel in nm und kDa. Die Molekülmassen sind Näherungswerte, da die Raumausdehnung von Makromolekülen (Huminstoffe) von der Molekülstruktur und den Umgebungsbedingungen abhängt. Die Werte für 0.45 µm (Filtration) und 2 µm (Sedimentation) sind als operationell definierte Trenngrenzen angegeben.

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Fast molecular mass and size characterization of polysaccharides using asymmetrical flow field-flow fractionation-multiangle light scattering

Cited by 109 publications

References 21 publications

Employment of detergent–tag/solute interactions in capillary electrophoresis of neutral polysaccharides

Employment of detergent–tag/solute interactions in capillary electrophoresis of neutral polysaccharides

Size Separation Techniques for the Characterisation of Cross-Linked Casein: A Review of Methods and Their Applications

Colloidal Particles in Sediment Pore Waters: Particle Size Distributions and Associated Element Size Distribution in Anoxic and Re‐oxidized Samples, Obtained by FFF‐ICP‐MS Coupling

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