Fast Sampling, Analyses and Chemometrics for Plant Breeding: Bitter Acids, Xanthohumol and Terpenes in Lupulin Glands of Hops (<scp><i>Humulus lupulus</i></scp>)

Killeen, Daniel P.; Watkins, Oliver C; Sansom, Catherine E.; Andersen, David H.; Gordon, Keith C.; Perry, Nigel B.

doi:10.1002/pca.2642

Cited by 29 publications

(17 citation statements)

References 42 publications

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“…Multivariate data analysis has already been applied in the quality control of hop, mostly for authenticity control [14,15]. Ocvirk et al used multivariate cluster analysis (CA) for the varietal classification of five different hop varieties concerning the bitter acid and essential oil composition [14] that are known to be characteristic for each hop cultivar [12,13,16]. Kovačevič et al also used GC-FID combined with principal component analysis (PCA) and CA for the verification of hop varieties [15].…”

Section: Introductionmentioning

confidence: 99%

Gas-phase volatilomic approaches for quality control of brewing hops based on simultaneous GC-MS-IMS and machine learning

Brendel

Schwolow

Rohn³

et al. 2020

Anal Bioanal Chem

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For the first time, a prototype HS-GC-MS-IMS dual-detection system is presented for the analysis of volatile organic compounds (VOCs) in fields of quality control of brewing hop. With a soft ionization and drift time-based ion separation in IMS and a hard ionization and m/z-based separation in MS, substance identification in the case of co-elution was improved, substantially. Machine learning tools were used for a non-targeted screening of the complex VOC profiles of 65 different hop samples for similarity search by principal component analysis (PCA) followed by hierarchical cluster analysis (HCA). Partial least square regression (PLSR) was applied to investigate the observed correlation between the volatile profile and the α-acid content of hops and resulted in a standard error of prediction of only 1.04% α-acid. This promising volatilomic approach shows clearly the potential of HS-GC-MS-IMS in combination with machine learning for the enhancement of future quality assurance of hops.

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Section: Introductionmentioning

confidence: 99%

Gas-phase volatilomic approaches for quality control of brewing hops based on simultaneous GC-MS-IMS and machine learning

Brendel

Schwolow

Rohn³

et al. 2020

Anal Bioanal Chem

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“…In addition to beer production, they are also used to make liqueurs, oils, infusions, capsules, dragees or ointments [7,8]. Hops are also a source of lupulin -a substance with a sedative and hypnotic effect, from the surface of female cones, which fills the hops glandular hair -so-called hops glands [2,7,9]. Similar properties show farnesene, humulone and lupulone -used to treat mood disorders, including anxiety or insomnia [3,4].…”

Section: Introductionmentioning

confidence: 99%

Comparison of antioxidant activity of extracts of hop leaves harvested in different years

et al. 2019

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Introduction: Hop (Humulus lupulus L.) is a common plant in Europe, with many beneficial health effects. In addition to the use in brewing, hops are a valuable source of active substances used in conventional and folk medicine, such as humulones and lupulones, as well as antioxidants, including phenolic compounds.Objective: The aim of the study was to evaluate and compare the antioxidant activity of alcoholic extracts of fresh hop leaves collected in 2017 and 2018. Material and methods:The raw material consisting of fresh hop leaves was extracted using ultrasoundassisted extraction. Methyl, ethyl and isopropyl alcohol at three concentrations were used as extractants. The antioxidant activity of extracts was determined using DPPH and FRAP methods. Total phenolic content was evaluated using the Folin-Ciocalteu technique. Results:All the extracts showed antioxidant potential as well as the phenolic content. Regardless of the harvesting year and methods of evaluation, the highest antioxidant activity and the total polyphenol content were observed for extracts prepared in undiluted methanol, obtained during one hour lasting extraction. Conclusion:The results of the studies have suggested that hop leaves can be a potential source of healthpromoting antioxidants.

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“…Kompakte NMR‐Spektrometer nutzen niedrige Felder, sodass ihre Anwendung im Wesentlichen auf einfache Lösungen kleiner Moleküle beschränkt ist. Obgleich die vollständige Zuordnung jeder chemischen Gruppe in mittelgroßen Molekülen mit Tisch‐NMR‐Spektrometern kaum möglich ist, liefert die hohe Auflösung modernster Geräte dennoch Strukturinformationen für komplexe Alkaloide, Sekundärmetaboliten und kleine Proteine. Sogar im Urin können Metaboliten innerhalb von Minuten erfasst und wesentliche Resonanzen in Spektren zugeordnet werden, die bei einer Feldstärke von 1.5 T gemessen wurden, wenn man sich das Hochleistungs‐Shimmen zunutze macht, um den Fuß der Lösungsmittellinie zu schmälern (Abbildung a).…”

Section: Fourier‐nmrunclassified

NMR mit Tischgeräten und deren Anwendungen von der Materialwissenschaft bis zur organischen Chemie

Blümich

Singh

2017

Angewandte Chemie

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Die NMR‐Spektroskopie ist eine unverzichtbare Analysemethode. Bis vor kurzem erforderten die Geräte viel Platz, waren teuer und schwer zu bedienen. Dies hat sich mit der Einführung kompakter NMR‐Spektrometer geändert, die sich für die Analyse kleiner Moleküle auf der Laborbank eignen. Solche Spektrometer sind mit Permanentmagneten für 1H‐NMR‐Frequenzen zwischen 40 und 80 MHz ausgestattet. Erst seit wenigen Jahren gibt es kleine Permanentmagnete mit homogenen Magnetfeldern für hochaufgelöste Spektroskopie. Tischgeräte mit inhomogenen Feldern werden schon seit 40 Jahren zur Charakterisierung von Lebensmitteln und Materialien über Relaxations‐ und Diffusionsmessungen genutzt. Ähnliche NMR‐Geräte messen diese Parameter im Streufeld außerhalb des Magneten. Sie werden zur Untersuchung von Bohrlochwänden in Ölquellen sowie für zerstörungsfreie Analysenverwendet. Der gegenwärtige Stand von NMR‐Spektroskopie, ‐Bildgebung und ‐Relaxometrie wird hier anhand repräsentativer Beispiele beschrieben.

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Fast Sampling, Analyses and Chemometrics for Plant Breeding: Bitter Acids, Xanthohumol and Terpenes in Lupulin Glands of Hops (Humulus lupulus)

Cited by 29 publications

References 42 publications

Gas-phase volatilomic approaches for quality control of brewing hops based on simultaneous GC-MS-IMS and machine learning

Gas-phase volatilomic approaches for quality control of brewing hops based on simultaneous GC-MS-IMS and machine learning

Comparison of antioxidant activity of extracts of hop leaves harvested in different years

NMR mit Tischgeräten und deren Anwendungen von der Materialwissenschaft bis zur organischen Chemie

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