Fat harvesting site is an important determinant of proliferation and pluripotency of adipose-derived stem cells

Ardeshirylajimi, Abdolreza; Rafeie, Farjad; Zandi-Karimi, Ali; Jaffarabadi, Ghobad Asgari; Mohammadi‐Sangcheshmeh, Abdollah; Samiei, Rahmat; Toghdory, Abdolhakim; Seyedjafari, Ehsan; Hashemi, Seyed Mahmoud; Çınar, Mehmet Ulaş; Gastal, E.L.

doi:10.1016/j.biologicals.2015.11.001

Cited by 20 publications

(16 citation statements)

References 30 publications

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“…In addition, B-ADSCs’ significant enhanced osteogenic and adipogenic capability was confirmed by further mRNA expression levels analysis of bone related genes - namely, Alp, Runx2, Ocn, and osterix ( 27 ), and adipose related genes - namely, Ppar- γ , Cebp- α , and Fabp4. This difference was also mentioned in a study by Ardeshirylajimi et al ( 20 ), despite different species. Interestingly, we found B-ADSCs from young mice expressed higher levels of earlier phases of osteogenesis (Runx2 and Alp), while B-ADSCs from aged expressed higher levels of late osteogenesis genes, such as Ocn.…”

Section: Discussionsupporting

confidence: 67%

“…However, the differences between ADSCs from BAT (B-ADSCs) and ADSCs from WAT (W-ADSCs) are not well known. Only a few literatures revealed the differentiation of B-ADSCs and W-ADSCs ( 20 ), but the difference of their immunoregulation capacity is still unclear. Especially, it’s still unknown whether the capacities of B-ADSCs and W-ADSCs will change as age increases.…”

Section: Introductionmentioning

confidence: 99%

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Proliferation, Differentiation and Immunoregulatory Capacities of Brown and White Adipose-Derived Stem Cells from Young and Aged Mice

Yang

Dong

et al. 2020

IJSC

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Background and Objectives Adipose tissue is a source of mesenchymal stem cells, which have the potential to differentiate into various types of cells. Adipose-derived stem cells (ADSCs) are now recognized as an accessible, abundant, and reliable stem cells suitable for tissue engineering and regenerative medicine applications. However, few literatures gave a comprehensive report on the capacities of ADSCs harvested from different sites. Especially, the capacities of ADSCs from aged mice remained unclear. In this study, we investigated several main capacities of brown adipose derived stem cells (B-ADSCs) and white adipose derived stem cells (W-ADSCs) from both young and aged mice. Methods and Results When isolated from young mice, B-ADSCs showed a stronger proliferation rate and higher osteogenic, adipogenic and myocardial differentiation ability than W-ADSCs. Carboxy fluorescein diacetate succinimidyl ester (CFSE) labeling test suggested no significant difference in immunosuppression capacity between B-ADSCs and W-ADSCs. Similarly, no difference between these two were found in several immune related molecules, such as programmed death-ligand 1 (PD-L1), intercellular cell adhesion molecule (ICAM-1), vascular cell adhesion molecule (VCAM-1), inducible nitric oxide synthase (iNOS), tumour necrosis factor- α (TNF- α ), interleukin 10 (IL10), and suppressor of cytokine signaling 1 (socs1). When isolated from aged mice, B-ADSCs also showed a stronger proliferation rate and higher osteogenic, adipogenic and myocardial differentiation ability than W-ADSCs; however, it demonstrated an attenuated immunosuppression capacity compared to W-ADSCs. Conclusions In summary, our data showed that ADSCs’ characteristics were tissue source dependent and changed with age. It provided evidence for choosing the right tissue-specific ADSCs for clinical application and fundamental research.

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Section: Discussionsupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Proliferation, Differentiation and Immunoregulatory Capacities of Brown and White Adipose-Derived Stem Cells from Young and Aged Mice

Yang

Dong

et al. 2020

IJSC

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“…Calcium content or mineralization is another marker of osteogenesis but unlike the ALP activity, which is considered as an early marker of osteogenesis, mineralization is considered as a late marker of osteogenesis (Ardeshirylajimi et al, 2016[ 6 ]). As we have seen from the result, the amount of calcium deposited was significantly higher for WJ-PLLA nanofibers in comparison with TCP and PLL nanofibers in all the days tested, supporting the role of WJ in enhancement of osteogenic differentiation.…”

Section: Discussionmentioning

confidence: 99%

Osteogenic differentiation of mesenchymal stem cells cultured on PLLA scaffold coated with Wharton’s jelly

Ahmadi

Seyedjafari

Zargar

et al. 2017

EXCLI Journal; 16:Doc785; ISSN 1611-2156

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“…To adipose derived MSCs preparation, first, abdominal fat tissue was obtained from patients underlying liposuction surgery (Taleghani Hospital, Tehran, Iran) based on the medical ethics committee guidelines of Iranian Ministry of Health and transferred to the laboratory under aseptic and sterile condition by put in DMEM medium content antibiotics and antifungals. After that MSCs were isolated enzymatically from fat tissues and then characterized by differentiating to the adipocyte-like and osteoblast-like cells according to our previous studies (Ardeshirylajimi et al 2016;Ghiaee et al 2017). Flow cytometry analysis was also performed to more characterization of the isolated MSCs by its CD (cluster of differentiation) markers whiles MSCs was in passage two, such as CD45 as a negative marker and CD44 and CD73 as positive markers according to the previously reported (Piran et al 2017).…”

Section: Mesenchymal Stem Cells Isolation and Characterizationmentioning

confidence: 99%

Poly-phosphate increases SMC differentiation of mesenchymal stem cells on PLGA–polyurethane nanofibrous scaffold

et al. 2020

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The use of bioactive scaffolds in tissue engineering has a significant effect on the damaged tissue healing by an increase in speed and quality of the process. Herein, electrospinning was applied to fabricate composite nanofibrous scaffolds by Poly lactic-co-glycolic acid (PLGA) and Polyurethane (PU) with and without poly-phosphate (poly-P). Scaffolds were characterized morphologically by scanning electron microscope (SEM), and their biocompatibility was also investigated by SEM, protein adsorption, cell attachment and survival assays. The applicability of the scaffolds for bladder tissue engineering was also evaluated by culturing mesenchymal stem cells (MSCs) on the scaffolds and their differentiation into smooth muscle cell (SMC) was studied at the gene and protein levels. The results demonstrated that scaffold biocompatibility was increased significantly by loading poly-P. SMC related gene and protein expression level in MSCs cultured on poly-P-loaded scaffold was also increased significantly compared to those cells cultured on empty scaffold. It can be concluded that poly-P hasn't also increased scaffold biocompatibility, but also SMC differentiation potential of MSCs was also increased while cultured on the poly-P containing scaffold compared to the empty scaffold. Taken together, our study showed that PLGA-PU-poly-P alone and in combination with MSCs has a promising potential for support urinary bladder smooth muscle tissue engineering.

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Fat harvesting site is an important determinant of proliferation and pluripotency of adipose-derived stem cells

Cited by 20 publications

References 30 publications

Proliferation, Differentiation and Immunoregulatory Capacities of Brown and White Adipose-Derived Stem Cells from Young and Aged Mice

Proliferation, Differentiation and Immunoregulatory Capacities of Brown and White Adipose-Derived Stem Cells from Young and Aged Mice

Osteogenic differentiation of mesenchymal stem cells cultured on PLLA scaffold coated with Wharton’s jelly

Poly-phosphate increases SMC differentiation of mesenchymal stem cells on PLGA–polyurethane nanofibrous scaffold

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