Fate, Transport, and Biodegradation of Natural Estrogens in the Environment and Engineered Systems

Khanal, Samir Kumar; Xie, Bin; Thompson, Michael L.; Sung, Shihwu; Ong, Say Kee; Leeuwen, J. van

doi:10.1002/chin.200701268

Cited by 73 publications

(137 citation statements)

References 1 publication

Supporting

Mentioning

130

Contrasting

Order By: Relevance

“…Biodegradation has been considered a crucial mechanism for removing steroids from natural and engineered ecosystems (Johnson and Sumpter, 2001;Andersen et al, 2003;Khanal et al, 2006). Sewage treatment plants are essential in removing steroid hormones, as shown by a comparison between sewage influents and effluents .…”

Section: Introductionmentioning

confidence: 99%

Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment

et al. 2016

View full text Add to dashboard Cite

Steroid hormones, such as androgens, are common surface-water contaminants. However, literature on the ecophysiological relevance of steroid-degrading organisms in the environment, particularly in anoxic ecosystems, is extremely limited. We previously reported that Steroidobacter denitrificans anaerobically degrades androgens through the 2,3-seco pathway. In this study, the genome of Sdo. denitrificans was completely sequenced. Transcriptomic data revealed gene clusters that were distinctly expressed during anaerobic growth on testosterone. We isolated and characterized the bifunctional 1-testosterone hydratase/dehydrogenase, which is essential for anaerobic degradation of steroid A-ring. Because of apparent substrate preference of this molybdoenzyme, corresponding genes, along with the signature metabolites of the 2,3-seco pathway, were used as biomarkers to investigate androgen biodegradation in the largest sewage treatment plant in Taipei, Taiwan. Androgen metabolite analysis indicated that denitrifying bacteria in anoxic sewage use the 2,3-seco pathway to degrade androgens. Metagenomic analysis and PCR-based functional assays showed androgen degradation in anoxic sewage by Thauera spp. through the action of 1-testosterone hydratase/dehydrogenase. Our integrative 'omics' approach can be used for culture-independent investigations of the microbial degradation of structurally complex compounds where isotopelabeled substrates are not easily available.

show abstract

Section: Introductionmentioning

confidence: 99%

Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The more and more serious pollution of environmental estrogens (EEs) in foods and water has received considerable concerns [1,2]. Amongst the EEs, 17␤-estradiol (17␤-E 2 ) is quite important, and has been widely used in environment and foods owing to its potent curative effects [3,4].…”

Section: Introductionmentioning

confidence: 99%

Preparation of photonic-magnetic responsive molecularly imprinted microspheres and their application to fast and selective extraction of 17β-estradiol

Peng

Luo

Xiong

et al. 2016

Journal of Chromatography A

View full text Add to dashboard Cite

a b s t r a c tPhotonic-magnetic responsive molecularly imprinted microspheres (PM-MIMs) were prepared by seed polymerization, through suitable functionalization of magnetic nanoparticles for further coating with photoresponsive functional monomer and imprinted layers, and then were successfully applied to the fast and selective extraction of 17␤-estradiol (17␤-E 2 ) from real samples. The PM-MIMs possessed a sandwich micro-spherical structure containing Fe 3 O 4 core, SiO 2 middle layer, and MIPs shell with thickness of 25 nm. The PM-MIMs displayed excellent photoresponsive properties and could be rapidly separated from solutions under an external magnet. The PM-MIMs had specific affinity towards 17␤-E 2 with high adsorption capacity (Q max = 0.84 mg g −1 ) and fast binding kinetics (K d = 26.08 mg L −1 ). The PM-MIMs proved to be an ideal photoswitch with the ability of reversible uptake and release of 17␤-E 2 upon alternate 365 and 440 nm irradiation: 45.0% of 17␤-E 2 released from the PM-MIMs upon 365 nm irradiation, and 94.0% of the released 17␤-E 2 was rebound to the PM-MIMs at 440 nm. Accordingly, the PM-MIMs were applied for fast separation and extraction of 17␤-E 2 followed by HPLC-UV determination, presenting the low limit of detection (LOD, S/N = 3) and quantification (LOQ, S/N = 10) of 0.18 and 0.62 mol L −1 , respectively. The high recoveries for spiked milk powder and drinking water samples were in the range of 97.5-113.0% with relative standard deviations less than 4.4%. This study reasonably combined photonic response, magnetic separation and surface imprinting, which endowed the PM-MIMs with significant advantages of high adsorption capacity and fast binding kinetics, convenient separation and recycled use, and simple rapid eco-benign adsorption/elution processes for template molecules. Thus, the PM-MIMs based method may be a simple, rapid, convenient, cost-effective and environmentally-friendly way for simultaneous separation, enrichment and detection of trace 17␤-E 2 in complicated matrices.

show abstract

“…This is equivalent to an influx into the environment of about 3.6 × 10 9 kg nitrogen and approximately 3.5 × 10 11 kg organic carbon each day. Compared to the smaller operations, CAFOs have limited land available for effective use/storage of the generated waste as fertilizer [3]. In addition, CAFOs do not require treatment of manure as long as it is not disposed directly into water bodies [2].…”

Section: Introductionmentioning

confidence: 99%

Aerobic and Anaerobic Transformations in Estrogens and Nutrients in Swine Manure: Environmental Consequences

Singh

2015

Agriculture

View full text Add to dashboard Cite

Pig manure is an excellent fertilizer and rich source of organic carbon and nitrogen compounds such organic nitrogen (O-N) (95% of total nitrogen) that is plant-unavailable-nitrogen (PUN) and mineralized nitrogen (about 1% of total nitrogen) such as ammonium (NH4 + ) and nitrate (NO3) that are plant-available-nitrogen (PAN). In addition, manure also contains two forms of estrogens: (i) poorly estrogenic thus essentially nontoxic conjugated estrogens (cEs) such as estrone (cE1), estradiol (cE2) and estriol (cE3); and (ii) highly estrogenic and toxic free estrogens (fEs) such as fE2, fE1 and fE3. This study showed that aerobic processing reduced concentrations of total carbon (TC), O-N, PAN and NH4 + /NH3 ratio, transiently hydrolyzed cEs (cE2 > cE1 > cE3) into corresponding fEs, transiently increased estrogenic activity and potential toxicity, and rapidly degraded fEs (fE2, fE1 > fE3), thus reducing the estrogenic activity in manure. Unlike aerobic processing, anaerobic processing stabilized and increased PAN and NH4 + /NH3 ratio, thus increasing the manure's fertilizer value. However, anaerobic processing, relative to aerobic processing, poorly hydrolyzed cEs (reducing transient toxicity and increasing reserve toxicity potential) and poorly degraded fEs (increasing toxicity) in manure. Thus, aerobic and anaerobic environments have distinct effects on manures' PAN and estrogenic activity, presenting an interesting dilemma: anaerobic incubation that increases manures' PAN does not effectively degrade estrogens, while aerobic incubation that effectively degrades estrogens (after transiently increasing their estrogenic activity) also decreases PAN, thus making manure less profitable. New techniques are need to fully use manure as organic fertilizer. OPEN ACCESSAgriculture 2015, 5 698

show abstract

Fate, Transport, and Biodegradation of Natural Estrogens in the Environment and Engineered Systems

Abstract: ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.

Cited by 73 publications

References 1 publication

Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment

Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment

Preparation of photonic-magnetic responsive molecularly imprinted microspheres and their application to fast and selective extraction of 17β-estradiol

Aerobic and Anaerobic Transformations in Estrogens and Nutrients in Swine Manure: Environmental Consequences

Contact Info

Product

Resources

About