Fatty acid analysis, cytotoxicity, antimicrobial and antioxidant activities of different extracts of the flowers of Nyctanthes arbor-tristis L.

Abstract

The fatty acid analysis and biological activity of n-hexane, dichloromethane, ethyl acetate and methanol extracts of Nyctanthes arbor-tristis L. flowers are reported. Five fatty acids namely palmitic (44.15%), stearic (19.34%), arachidic (15.06%), behenic (9.77%) and lignoceric (11.69%) acids were identified. From cytotoxicity test, the LC50 values (the median lethal concentration) for n-hexane, dichloromethane, ethyl acetate and methanol extracts as well as for standard vincristine sulphate were found 7.05, 4.67, 3.14, 5.53 and 0.50 μg/ml, respectively. Antibacterial activity results of different extracts were compared with standard antibiotic ciprofloxacin and tetracycline by measuring the zone of inhibition diameter in millimeter. The dichloromethane and ethyl acetate extracts showed significant antibacterial activity. From antioxidant activity test, IC50 values (50% inhibitory concentration) of n-hexane, dichloromethane, ethyl acetate, methanol extracts and ascorbic acid were found to be 291.92 mg/ml, 45.74 μg/ml, 21.86 μg/ml, 64.30 μg/ml and 3.98 μg/ml, respectively.
 Bangladesh J. Sci. Ind. Res.55(3), 207-214, 2020

Biomedical Applications Of Novel Green Biomaterial Synthesized From Endophytic Bacteria Cronobacter Sakazakii

“…In MIC assay, as shown in g. 3a, CSB inhibited the growth of E.coli strains when compared to the standard drug Ampicillin. This study is in agreement with ndings that showed the antibacterial activity of the owers of NAT [35][36][37]. The lowest concentration at which visible growth of Gram negative human bacteria E.coli strains 1 and 2, ATCC E.coli and K.pneumonia were inhibited at 24 hours of culture was seen at 1.5625µg/ml, 12.5µg/ml, 25µg/ml and 6.25µg/ml which gave percentage inhibition of 47.5%, 65.3%,57.1% and 55.6% respectively.…”

“…Natural plants are known to possess polyphenolic compounds responsible for antioxidant activity and previous phytochemical screening showed the presence of these polyphenolic compounds in the owers of NAT [37]. Different ower extracts of NAT has also showed antioxidant activity [37,43]. These bioactive compounds in and endophytes can donate hydrogen to free radicals thereby disrupt the free radical chain reaction by acting as CSB-capping [44], making the synthesized CSB served as hydrogen donors and reducing agents as seen in this study.…”

“…When compared to the wells containing standard drug, E2, K.pneumoniae, and all S1 strains were completely resistant (R) to the standard drug hence no inhibition was observed. Previous studies have assessed antibacterial potential of the owers of NAT in terms of zone of inhibition using various extracts but at higher concentrations [35][36][37]. Therefore synthesized CSB can be considered as a potent antimicrobial agent as it is able to inhibit the growth of both positive and negative Gram bacteria.…”

“…Natural plants are known to possess polyphenolic compounds responsible for antioxidant activity and previous phytochemical screening showed the presence of these polyphenolic compounds in the owers of NAT [37]. Different ower extracts of NAT has also showed antioxidant activity [37,43].…”