Fatty acid and lipid composition of 10 species of microalgae used in mariculture

Volkman, John K.; Jeffrey, S. W.; Nichols, Peter D.; Rogers, Glen I.; Garland, C. D.

doi:10.1016/0022-0981(89)90029-4

Cited by 940 publications

(597 citation statements)

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“…Biomarker analysis of the separated algal fraction further suggested that a significant fractionation of the MPB occurred using the lens-tissue method. Diatom markers, including the PUFA 20:5(n-3) and sterol 24-methylcholesta-5, 22E-dien-3β-ol, were not detected at all (Table 5), nor were the green algae fatty acid markers including 18:2(n-6) and 18:1(n-9) (Volkman et al 1989(Volkman et al , 1998. These markers were all detected in the sediments throughout the year, further indicating that the separated fraction of MPB was depleted in diatoms and chlorophytes compared to that remaining within the sediment.…”

Section: Fractionation Of Mpb Using the Lens-tissue Methodsmentioning

confidence: 99%

“…Therefore, it would appear that in this system 24-methylcholesta-5, 24(28)-dien-3β-ol is likely to arise mostly from diatoms. The presence of chlorophytes was indicated by the fatty acids 18:2(n-6) and 18:3(n-3) (Volkman et al 1980(Volkman et al , 1989, particularly at Site PC, where the pigment data suggested these algae were generally dominant. Although there are no generally accepted fatty acid markers for cyanobacteria, the 18:4 PUFA was present in relatively high proportions in the extract of separated MPB dominated by cyanobacteria.…”

Section: Lipids and Pigments As Indicators Of Mpb Taxonomymentioning

confidence: 96%

“…Volkman et al (1980). The PUFA 22:6(n-3) was also clustered on the periphery of the algal markers, most probably reflecting the fact that this fatty acid is produced by both microalgae (Volkman et al 1989) and sediment infauna (Kharlamenko et al 2001).The second cluster of fatty acids consisted predominantly of odd-chain fatty acids including the widely used bacterial markers i15:0 and ai15:0 (Findlay & Dobbs 1993), strongly suggesting that the fatty acids within this group were predominantly derived from bacterial sources. The unusual fatty acids 15:0 and 17:1(n-8) were present in high relative proportions at 66 Cook at al.…”

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confidence: 94%

“…Fatty acids also offer the potential to distinguish various algal classes (Volkman et al 1989(Volkman et al , 1998, although these may be less specific than pigments. Using these methods to estimate the relative importance of algal classes requires an estimate to be made of the ratios of pigments and fatty acids to a measure of biomass such as chlorophyll a (chl a).…”

Section: Introductionmentioning

confidence: 99%

“…and had a measured ratio to chl a of 0.3 (Table 5). It was assumed that the contribution of 18:4 by chlorophytes (which are known to produce C 18 65 PUFAs) was negligible, since previous studies have shown that chlorophytes only produce small amounts of 18:4 compared to total lipids (~3%) (Volkman et al 1989, Ahlgren et al 1992). These 2 approaches were generally in broad agreement ( Table 6), suggesting that Oscillatoria spp.…”

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confidence: 99%

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Carbon and nitrogen cycling on intertidal mudflats of a temperate Australian estuary. III. Sources of organic matter

Cook¹,

Revill²,

Clementson³

et al. 2004

Mar. Ecol. Prog. Ser.

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The sources and cycling of organic matter on 2 mudflats in the Huon Estuary, SE Tasmania, were investigated over 4 seasons using pigment and lipid biomarkers as well as stable isotopes of carbon and nitrogen; 1 site was in the upper estuary,1 site was in a marine side-arm. The organic matter within the sediments at both sites was dominated by terrestrial sources, but most of this was refractory and only slowly remineralised. Significant, but seasonally variable, amounts of organic matter were derived from microphytobenthos (MPB), but this was rapidly remineralised and comprised only a minor fraction of the preserved sediment organic matter pool. Compound specific stable-isotope analysis of bacterial biomarkers suggested that the bacteria within the sediment were consuming microalgae-derived material. Separation of MPB from the sediment at the site in the upper estuary using the lens-tissue technique was found to significantly fractionate the algae community with cyanobacteria being enriched in the separated fraction. Nitrogen fixation probably contributed significantly to the growth requirements of cyanobacteria at the site in the upper estuary, as indicated by the low δ 15 N value of the cyanobacteria separated from the sediment.KEY WORDS: Microphytobenthos · Stable isotope · Compound-specific isotope-ratio mass spectrometry · Fatty acids · Sterols · Accessory pigment · Mudflat Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 280: [55][56][57][58][59][60][61][62][63][64][65][66][67][68][69][70][71][72] 2004 1999, Cahoon & Safi 2002), but this may shift seasonally to chlorophytes and cyanobacteria (Barranguet et al. 1997). In some areas, intertidal sediments may be dominated by cyanobacteria where high rates of nitrogen fixation may supply a significant amount of nitrogen to the benthic community (Stal et al. 1984, Bautista & Paerl 1985, Stal 1995.A number of approaches have been used to estimate the contributions of organic matter from various sources to sediments. Isotope ratios of 13 C/ 12 C have been used to estimate the relative contribution of terrestrial and marine sources to sedimentary organic matter (Fry & Sherr 1984). While this approach is relatively simple and gives an integrated estimate of sources for the total carbon in the sample, it will only provide useful information when there are 2 welldefined end-members. Furthermore, this technique gives little information about the type of marine or terrestrial organic matter in question.The use of lipid and pigment biomarkers such as fatty acids and sterols can allow the sources of various subfractions of organic matter to be identified much more specifically than any of the above approaches. However, unambiguously assigning sources to biomarkers is difficult, as they are rarely produced by a single class of organism. The advent of compound-specific stableisotope analysis has provided a powerful new tool for the differentiation between 2 sources of the same biomarker, provided the isotopic sign...

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Section: Fractionation Of Mpb Using the Lens-tissue Methodsmentioning

confidence: 99%

Section: Lipids and Pigments As Indicators Of Mpb Taxonomymentioning

confidence: 96%

mentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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