Fatty acid and lipid composition in mycelia from submerged or surface culture of Streptomyces viridochromogenes

Shim, M

doi:10.1016/0378-1097(93)90479-l

Cited by 2 publications

(2 citation statements)

References 4 publications

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“…One of the most relevant changes was the disappearance of PE, and its apparent substitution with OL in samples taken at the later stages of development. It had been previously reported that OL was accumulated in Streptomyces viridochromogenes after a long incubation time, and that this effect was associated with P i concentration in culture medium ( Shim and Kim, 1993 ), however, the complete replacement of PE observed in our results ( Figures 3C and 5 ) is much more drastic than what had been reported in other actinomycetes. We show that OL accumulation is induced by phosphate limitation.…”

Section: Discussioncontrasting

confidence: 67%

Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development

et al. 2015

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Streptomyces coelicolor is a model actinomycete that is well known for the diversity of its secondary metabolism and its complex life cycle. As a soil inhabitant, it is exposed to heterogeneous and frequently changing environmental circumstances. In the present work, we studied the effect of diverse growth conditions and phosphate depletion on its lipid profile and the relationship between membrane lipid composition and development in S. coelicolor. The lipid profile from cultures grown on solid media, which is closer to the natural habitat of this microorganism, does not resemble the previously reported lipid composition from liquid grown cultures of S. coelicolor. Wide variations were also observed across different media, growth phases, and developmental stages indicating active membrane remodeling. Ornithine lipids (OL) are phosphorus-free polar lipids that were accumulated mainly during sporulation stages, but were also major components of the membrane under phosphorus limitation. In contrast, phosphatidylethanolamine, which had been reported as one of the major polar lipids in the genus Streptomyces, is almost absent under these conditions. We identified one of the genes responsible for the synthesis of OL (SCO0921) and found that its inactivation causes the absence of OL, precocious morphological development and actinorhodin production. Our observations indicate a remarkable plasticity of the membrane composition in this bacterial species, reveal a higher metabolic complexity than expected, and suggest a relationship between cytoplasmic membrane components and the differentiation programs in S. coelicolor.

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Section: Discussioncontrasting

confidence: 67%

Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development

et al. 2015

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“…This has been shown for S. coelicolor , S. cerevisiae , B. subtilis , and Bacillus megaterium , which were more resistant to H 2 O 2 or high temperature stress the higher the content of saturated fatty acid in their membrane lipids was [68] – [69] . For S. viridochromogenes it is known that fatty acids composition of their membrane lipids has a crucial effect on membrane rigidity and permeability [70] . Our data of the changed expression of proteins contributing to fatty acids metabolism also may reflect the importance of membrane lipid composition in oxidative stress response of S. viridochromogenes .…”

Section: Resultsmentioning

confidence: 99%

Proteomic Approach to Reveal the Regulatory Function of Aconitase AcnA in Oxidative Stress Response in the Antibiotic Producer Streptomyces viridochromogenes Tü494

Michta

Ding²,

Zhu³

et al. 2014

PLoS ONE

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The aconitase AcnA from the phosphinothricin tripeptide producing strain Streptomyces viridochromogenes Tü494 is a bifunctional protein: under iron-sufficiency conditions AcnA functions as an enzyme of the tricarboxylic acid cycle, whereas under iron depletion it is a regulator of iron metabolism and oxidative stress response. As a member of the family of iron regulatory proteins (IRP), AcnA binds to characteristic iron responsive element (IRE) binding motifs and post-transcriptionally controls the expression of respective target genes. A S. viridochromogenes aconitase mutant (MacnA) has previously been shown to be highly sensitive to oxidative stress. In the present paper, we performed a comparative proteomic approach with the S. viridochromogenes wild-type and the MacnA mutant strain under oxidative stress conditions to identify proteins that are under control of the AcnA-mediated regulation. We identified up to 90 differentially expressed proteins in both strains. In silico analysis of the corresponding gene sequences revealed the presence of IRE motifs on some of the respective target mRNAs. From this proteome study we have in vivo evidences for a direct AcnA-mediated regulation upon oxidative stress.

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Fatty acid and lipid composition in mycelia from submerged or surface culture of Streptomyces viridochromogenes

Cited by 2 publications

References 4 publications

Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development

Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development

Proteomic Approach to Reveal the Regulatory Function of Aconitase AcnA in Oxidative Stress Response in the Antibiotic Producer Streptomyces viridochromogenes Tü494

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