Fatty acid binding protein expression in different adipose tissue depots from lean and obese individuals

Fisher, Rachel M.; Eriksson, Per; Hoffstedt, Johan; Hotamisligil, G S; Thörne, A.; Rydén, Mikael; Hamsten, Anders; Arner, Peter

doi:10.1007/s001250100643

Cited by 62 publications

(44 citation statements)

References 16 publications

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“…However, its mRNA was later found to be expressed in various tissues including adipose tissue 5,8,9,[12][13][14][15][16][17][18][19][20][21][22][23] . Krieg et al 5) examined levels of C-FABP mRNA in mouse tissues, and found that the mRNA was most abundant in stratified epithelia of the epidermis and tongue.…”

Section: Discussionmentioning

confidence: 99%

Modulation of Cutaneous Fatty Acid-binding Protein mRNA Expression in Rat Adipose Tissues by Hereditary Obesity and Dietary Fats

2007

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Section: Discussionmentioning

confidence: 99%

Modulation of Cutaneous Fatty Acid-binding Protein mRNA Expression in Rat Adipose Tissues by Hereditary Obesity and Dietary Fats

2007

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“…Actin is perhaps the most commonly used protein in this respect and we ourselves have used it in a recent publication. 18 In that study we compared actin expression within, rather than between, subjects with similar BMI and found only small variations in actin expression. However, here we show that there are substantial differences in the expression levels of actin in subcutaneous adipose tissue from lean and obese individuals.…”

Section: Discussionmentioning

confidence: 99%

“…18 Such differences in expression pattern might be involved in determining the different basal (non-hormonally stimulated) rates of lipolysis observed in these depots. 21 The ALBP=KLBP ratio was higher in subcutaneous compared to visceral fat from both lean and obese subjects.…”

Section: Discussionmentioning

confidence: 99%

“…Before surgery, a venous blood sample was taken from the subjects following an overnight fast for measurement of plasma glucose, triacylglycerol, NEFA and cholesterol concentrations by the hospital routine chemistry laboratory and serum insulin by radio-immunoassay (Amersham Pharmacia, Uppsala, Sweden). For a subset of subjects mean cell volumes of isolated adipocytes were determined 18 as follows: lean 500 AE 46 (n ¼ 9); obese 900 AE 40 (n ¼ 11); weight-reduced 543 AE 54 (n ¼ 12) pl (P 0.0001 for obese vs both lean and weight-reduced subjects). The study was approved by the Local Ethics Committee of Karolinska Institutet (Stockholm, Sweden).…”

Section: Subjectsmentioning

confidence: 99%

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Effects of obesity and weight loss on the expression of proteins involved in fatty acid metabolism in human adipose tissue

et al. 2002

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OBJECTIVE: Disturbances in adipocyte lipolysis in obesity may contribute to elevated circulating non-esterified fatty acid (NEFA) concentrations and insulin resistance. In experimental models, NEFA metabolism is influenced by adipocyte proteins such as adipocyte and keratinocyte lipid binding proteins (aP2=ALBP and mal1=KLBP) and fatty acid translocase (CD36). We investigated the effect of obesity and weight loss on the expression of these proteins in human subcutaneous adipose tissue. STUDY DESIGN AND SUBJECTS: Subcutaneous adipose tissue was obtained from 12 obese (body mass index (BMI) 42.4 AE 1.6 kg=m 2 ) and 12 lean (23.4 AE 0.6 kg=m 2 ) subjects. The obese subjects underwent gastric banding and biopsies were taken again after 2 y following a significant weight reduction (BMI 32.8 AE 1.4 kg=m 2 ). Adipose tissue proteins were quantified by Western blotting. RESULTS: Differential expression of ALBP, KLBP and CD36 was observed in lean and weight-reduced subjects compared with obese individuals. This resulted in a significantly lower ALBP=KLBP ratio in lean and weight-reduced individuals compared to obese subjects. Furthermore there was a significant influence of gender on this ratio. Moreover, the commonly used internal standard protein actin was expressed significantly higher in lean compared to obese individuals. CONCLUSION: The relative content of ALBP and KLBP in human adipose tissue changes with obesity, weight loss and gender indicating differential regulation. Differing responses in the expression patterns of adipose tissue proteins capable of binding NEFAs in response to weight changes suggest a potential importance in the development of obesity-associated complications.

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“…Moreover, insulin-sensitising and pro-adipogenic peroxisome proliferator-activated receptor (PPAR)γ agonists induce AFABP production and secretion [12,13]. In addition, AFABP mRNA and protein production are elevated in subcutaneous adipose tissue compared with the visceral depot in lean and obese humans [14].…”

Section: Production and Structure Of Afabpmentioning

confidence: 99%

Adipocyte fatty acid binding protein: a novel adipokine involved in the pathogenesis of metabolic and vascular disease?

2012

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Adipocyte fatty acid binding protein (AFABP, also known as aP2 and FABP4) has recently been introduced as a novel fat-derived circulating protein. AFABP serum concentrations are positively correlated with markers of the metabolic syndrome and vascular disease in various cross-sectional and interventional studies. Furthermore, a small set of prospective studies indicates that high AFABP serum levels at baseline predict the risk for metabolic and vascular morbidity and mortality. Studies in Afabp (also known as Fabp4) knockout mice and AFABP inhibitortreated animals suggest that total AFABP promotes insulin resistance, hypertriacylglycerolaemia and atherosclerosis by ligand/ligand delivery, as well as ligand-independent mechanisms. In contrast, the pathophysiological significance of circulating AFABP and the mechanisms leading to its release remain to be established. The current review summarises recent findings on the regulation and potential role of AFABP in metabolic and vascular disease.

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Fatty acid binding protein expression in different adipose tissue depots from lean and obese individuals

Cited by 62 publications

References 16 publications

Modulation of Cutaneous Fatty Acid-binding Protein mRNA Expression in Rat Adipose Tissues by Hereditary Obesity and Dietary Fats

Modulation of Cutaneous Fatty Acid-binding Protein mRNA Expression in Rat Adipose Tissues by Hereditary Obesity and Dietary Fats

Effects of obesity and weight loss on the expression of proteins involved in fatty acid metabolism in human adipose tissue

Adipocyte fatty acid binding protein: a novel adipokine involved in the pathogenesis of metabolic and vascular disease?

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