Fatty acid composition in lipid fractions lengthwise the mycelium of Mortierella isabellina and lipid production by solid state fermentation

Fakas, Stylianos; Makri, Anna; Mavromati, Maria; Tselepi, Maria; Aggelis, George

doi:10.1016/j.biortech.2009.06.015

Cited by 101 publications

(64 citation statements)

References 8 publications

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“…A similar trend was reported for M. isabellina grown on pear pomace [18], T. elegans grown on sugars [32], and C. echinulata grown on tomato wastes [36]. However, in the present research the opposite situation was observed in the cases of C. echinulata and Z. moelleri.…”

Section: Fatty Acid Composition Of Lipid Fractionssupporting

confidence: 82%

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Lipids Containing Polyunsaturated Fatty Acids Synthesized by Zygomycetes Grown on Glycerol

Bellou

Moustogianni

Makri

et al. 2011

Appl Biochem Biotechnol

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104

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Several strains of Zygomycetes cultivated on glycerol produced mycelia rich in lipids containing higher amounts of neutral lipids (NL) than glycolipids plus sphingolipids and phospholipids (P), while biosynthesis of P in Mortierella ramanniana, Mucor sp., and Cunninghamella echinulata occurred though NL accumulation process was in progress. Polyunsaturated fatty acids (PUFA) concentration gradually decreased in all lipid fractions of M. ramanniana during growth. In contrast, in C. echinulata concentration of both linoleic and γ-linolenic acids increased with time, especially in P. Taking for granted that the main function of PUFA is associated to their participation in mycelial membranes, we could suppose that biosynthesis of these fatty acids is associated to mycelial growth. However, this is accurate only for some Zygomycetes, e.g., M. ramanniana. On the contrary, PUFA biosynthesis in C. echinulata persists after growth cessation, suggesting that in this species biosynthetic ability is not a strictly growth-associated process. Phosphatidyl-inositol and phosphatidyl-choline were the major P classes in C. echinulata and M. ramanniana, respectively. In M. ramanniana, a decrease of PUFA concentration was noticed even when mycelia were incubated in low temperature (conditions that normally favor PUFA biosynthesis), indicating that PUFA biosynthesis in this fungus is associated to primary metabolism.

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Section: Fatty Acid Composition Of Lipid Fractionssupporting

confidence: 82%

“…The production cost of SCO is currently considered high, so substrates for cultivating oleaginous microorganisms of low acquisition cost, such as glycerol produced during biodiesel manufacture, as well as a number of agro-industrial residues, have attracted the interest of several research groups [16][17][18][19][20][21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Lipids Containing Polyunsaturated Fatty Acids Synthesized by Zygomycetes Grown on Glycerol

Bellou

Moustogianni

Makri

et al. 2011

Appl Biochem Biotechnol

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104

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“…There were no significant changes in fatty acid compositional profile among samples under different culture conditions. Generally speaking, palmitate, oleate and stearate were the three predominant components in lipid samples from oleaginous yeasts (Johnson et al, 1995), although slight change in relative contents could occur according to the cell age or culture conditions (Fakas et al, 2009). …”

Section: The Fatty Acid Composition Of the Lipid Samplesmentioning

confidence: 97%

Lipid production by Lipomyces starkeyi cells in glucose solution without auxiliary nutrients

Lin

Shen

Tan

et al. 2011

Journal of Biotechnology

125

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“…NL produced in NRM), during transition from asexual to sexual stage, linoleic acid concentration decreased markedly, mainly in P, while oleic acid concentration increased. Judging from the ratios C18 : 1/C18 : 0 and C18 : 2/C18 : 1, which may reflect the activity of the related desaturases (Fakas et al, 2009), we suggest that the growing vegetative cells demonstrated a high D12 desaturase activity that was gradually reduced as the sexual reproduction proceeded. Oleic acid (but not stearoyl-CoA) is desaturated after its esterification in P of endoplasmic reticulum.…”

Section: Quantitative and Compositional Shifts In Lipid Fractions Durmentioning

confidence: 99%

“…The activities of D9 and D12 desaturases were estimated by using the ratios D9 C18 : 1/ C18 : 0 and D9,12 C18 : 2 / D9 C18 : 1 respectively according to Fakas et al (2009).…”

mentioning

confidence: 99%

Fatty acid biosynthesis during the life cycle of Debaryomyces etchellsii

2016

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Fatty acid biosynthesis during the life cycle of the ascomycetous yeast Debaryomyces etchellsii cultivated on a non-fermentable substrate, i.e. glycerol, in nitrogen rich media (NRM) and nitrogen limited media (NLM) has been studied. Although considerable activities of key lipogenic enzymes, such as ATP citrate lyase (ACL) and malic enzyme (ME), were detected in vegetative cells during asexual proliferation (which occurred in the first growth stages in both NRM and NLM), lipid accumulation was restricted due to the high activities of NAD + -isocitrate dehydrogenase (NAD + -ICDH). A similar enzymatic profile has been found in ascii and free ascospores produced in NRM; thus lipid accumulation was low. On the contrary, very high activities of both ACL and ME and low activities of NAD + -ICDH were detected in ascii and free ascospores produced in NLM resulting in lipid accumulation. Neutral lipids (NL) were the predominant fraction of cellular lipids produced in vegetative cells and ascospores in both NRM and NLM. On the other hand, phospholipids (P) were the major polar lipids while glycolipids (G) were synthesized in low proportions. During transition from asexual to sexual phase, the percentage of NL increased with a significant decrease of P and, to a lesser extent, of G. High quantities of linoleic acid were found esterified in polar lipids, especially in P, during the vegetative stage of growth, while, with a few exceptions, during transition from asexual to sexual stage, linoleic acid concentration decreased markedly, mainly in P, while oleic acid concentration increased.

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Fatty acid composition in lipid fractions lengthwise the mycelium of Mortierella isabellina and lipid production by solid state fermentation

Cited by 101 publications

References 8 publications

Lipids Containing Polyunsaturated Fatty Acids Synthesized by Zygomycetes Grown on Glycerol

Lipids Containing Polyunsaturated Fatty Acids Synthesized by Zygomycetes Grown on Glycerol

Lipid production by Lipomyces starkeyi cells in glucose solution without auxiliary nutrients

Fatty acid biosynthesis during the life cycle of Debaryomyces etchellsii

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