Fatty acid composition in seeds of holoparasitic Orobanchaceae from the Caucasus region: Relation to species, climatic conditions and nutritional value

Ruraż, Karolina; Piwowarczyk, Renata; Gajdoš, Peter; Krasylenko, Yuliya; Čertı́k, Milan

doi:10.1016/j.phytochem.2020.112510

Cited by 5 publications

(6 citation statements)

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“…The cutinized endothelium has a protective role in the underground part of the plant life cycle (Dinesh et al, 2015; Piwowarczyk et al, 2019). Lipids are the main storage material in the seeds of Orobanchaceae (Ruraż et al, 2020). For germination, Cistanche seeds need to be very nearby their preferred host.…”

Section: Introductionmentioning

confidence: 99%

Characterization and diversity of seed endophytic bacteria of the endemic holoparasitic plant Cistanche armena (Orobanchaceae) from a semi-desert area in Armenia

Petrosyan

Thijs²,

Piwowarczyk³

et al. 2022

Seed Sci. Res.

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We explored the seed-associated bacterial endophytic microbiome in seeds of the endemic holoparasitic species Cistanche armena from a saline and arid habitat in Armenia. A combination of culture-dependent and molecular techniques was employed for identifying the seed endomicrobiome (culturable and unculturable). From surface-sterilized seeds, 10 phyla, comprising 256 endophytic bacterial genera, were identified. Of the culturable strains, we also investigated the plant growth-promoting (PGP) traits. Most of the isolates were spore forming, halotolerant and alkaliphile Bacillus spp., indicating that the endophytic bacteria of C. armena seeds own traits related to the natural habitat of their host plant. Our results confirm that Bacillus species are common and dominated endophytes from plants growing on saline and arid soils. Pantoea spp. and Stenotrophomonas spp. are more favourable PGP endophytes in seeds of C. armena. The PGP traits of these bacteria, such as production of indole, a precursor of auxin, ACC-deaminase and organic acids have the potential to improve the tolerance of their host plants against the abiotic stresses present in their natural habitat. To the best of our knowledge, this is the first report concerning bacterial seed endophytes of the C. armena.

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Section: Introductionmentioning

confidence: 99%

Characterization and diversity of seed endophytic bacteria of the endemic holoparasitic plant Cistanche armena (Orobanchaceae) from a semi-desert area in Armenia

Petrosyan

Thijs²,

Piwowarczyk³

et al. 2022

Seed Sci. Res.

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“…Lipids in the seed of plant are crucial in controlling seed dormancy and growth, dispersal and defenses against biotic and abiotic stress [ 35 ]. Its biosynthesis and accumulation are affected by various natural environmental conditions.…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of metabolome, transcriptome, and bioclimatic factors of Acer truncatum seeds reveals key candidate genes related to unsaturated fatty acid biosynthesis, and potentially optimal production area

Li,

Kong,

et al. 2024

BMC Plant Biol

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Background Lipids found in plant seeds are essential for controlling seed dormancy, dispersal, and defenses against biotic and abiotic stress. Additionally, these lipids provide nutrition and energy and are therefore important to the human diet as edible oils. Acer truncatum, which belongs to the Aceaceae family, is widely cultivated around the world for its ornamental value. Further because its seed oil is rich in unsaturated fatty acids (UFAs)- i.e. α-linolenic acid (ALA) and nervonic acid (NA)- and because it has been validated as a new food resource in China, the importance of A. truncatum has greatly risen. However, it remains unknown how UFAs are biosynthesized during the growth season, to what extent environmental factors impact their content, and what areas are potentially optimal for their production. Results In this study, transcriptome and metabolome of A. truncatum seeds at three representative developmental stages was used to find the accumulation patterns of all major FAs. Cumulatively, 966 metabolites and 87,343 unigenes were detected; the differential expressed unigenes and metabolites were compared between stages as follows: stage 1 vs. 2, stage 1 vs. 3, and stage 2 vs. 3 seeds, respectively. Moreover, 13 fatty acid desaturases (FADs) and 20 β-ketoacyl-CoA synthases (KCSs) were identified, among which the expression level of FAD3 (Cluster-7222.41455) and KCS20 (Cluster-7222.40643) were consistent with the metabolic results of ALA and NA, respectively. Upon analysis of the geographical origin-affected diversity from 17 various locations, we found significant variation in phenotypes and UFA content. Notably, in this study we found that 7 bioclimatic variables showed considerable influence on FAs contents in A. truncatum seeds oil, suggesting their significance as critical environmental parameters. Ultimately, we developed a model for potentially ecological suitable regions in China. Conclusion This study provides a comprehensive understanding of the relationship between metabolome and transcriptome in A. truncatum at various developmental stages of seeds and a new strategy to enhance seed FA content, especially ALA and NA. This is particularly significant in meeting the increasing demands for high-quality edible oil for human consumption. The study offers a scientific basis for A. truncatum’s novel utilization as a woody vegetable oil rather than an ornamental plant, potentially expanding its cultivation worldwide.

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“…loss of the roots and plastids 9 , 10 , and the production of large numbers of extremely small seeds which remain viable in the soil for many years 11 , 12 . The main storage material in the seeds of holoparasitic Orobanchaceae are lipids 13 . The crucial stages of the lifecycle of holoparasitic plants are seed germination, reaching the desirable host, gaining access to nutrients, and development of the new generation 14 .…”

Section: Introductionmentioning

confidence: 99%

Diversity and potential plant growth promoting capacity of seed endophytic bacteria of the holoparasite Cistanche phelypaea (Orobanchaceae)

Petrosyan

Thijs

Piwowarczyk

et al. 2023

Sci Rep

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Salt marshes are highly dynamic, biologically diverse ecosystems with a broad range of ecological functions. We investigated the endophytic bacterial community of surface sterilized seeds of the holoparasitic Cistanche phelypaea growing in coastal salt marshes of the Iberian Peninsula in Portugal. C. phelypaea is the only representative of the genus Cistanche that was reported in such habitat. Using high-throughput sequencing methods, 23 bacterial phyla and 263 different OTUs on genus level were found. Bacterial strains belonging to phyla Proteobacteria and Actinobacteriota were dominating. Also some newly classified or undiscovered bacterial phyla, unclassified and unexplored taxonomic groups, symbiotic Archaea groups inhabited the C. phelypaea seeds. γ-Proteobacteria was the most diverse phylogenetic group. Sixty-three bacterial strains belonging to Bacilli, Actinomycetes, α-, γ- and β-Proteobacteria and unclassified bacteria were isolated. We also investigated the in vitro PGP traits and salt tolerance of the isolates. Among the Actinobacteria, Micromonospora spp. showed the most promising endophytes in the seeds. Taken together, the results indicated that the seeds were inhabited by halotolerant bacterial strains that may play a role in mitigating the adverse effects of salt stress on the host plant. In future research, these bacteria should be assessed as potential sources of novel and unique bioactive compounds or as novel bacterial species.

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Fatty acid composition in seeds of holoparasitic Orobanchaceae from the Caucasus region: Relation to species, climatic conditions and nutritional value

Cited by 5 publications

References 50 publications

Characterization and diversity of seed endophytic bacteria of the endemic holoparasitic plant Cistanche armena (Orobanchaceae) from a semi-desert area in Armenia

Characterization and diversity of seed endophytic bacteria of the endemic holoparasitic plant Cistanche armena (Orobanchaceae) from a semi-desert area in Armenia

Integrated analysis of metabolome, transcriptome, and bioclimatic factors of Acer truncatum seeds reveals key candidate genes related to unsaturated fatty acid biosynthesis, and potentially optimal production area

Diversity and potential plant growth promoting capacity of seed endophytic bacteria of the holoparasite Cistanche phelypaea (Orobanchaceae)

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