Fatty acid composition of vegetable oil blend and in vitro effects of pharmacotherapeutical skin care applications

Abstract

Vegetable oils have been used for a plethora of health benefits by their incorporation in foods, cosmetics, and pharmaceutical products, especially those intended for skin care. This study aimed to investigate the cutaneous benefits of a vegetable oil blend (VOB) formulation and its fatty acid composition. The anti-inflammatory activity was studied in macrophages of RAW 264.7 cells by investigating the release of nitric oxide (NO), superoxide anion generation (O 2 - ), tumor necrosis factor-alpha (TNF-α), and interleukin 6 (IL-6). ABTS cation radical scavenging capacity assay, ferric reducing antioxidant potential (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and NO free radical scavenging assays were used to evaluate the antioxidant activity. VOB was tested for its ability to stimulate fibroblast proliferation and migration using the scratch assay, and antibacterial activity by the microdilution test. The fatty acid profile of a freshly prepared VOB formulation was determined by gas chromatography before and after accelerated stability testing. Chemical composition of VOB revealed the presence of oleic acid (C18:1n-9; 63.3%), linoleic acid (C18:2n-6; 4.7%), and linolenic acid (C18:3n-6; 5.1%) as major mono- and polyunsaturated fatty acids. No changes in the organoleptic characteristics and fatty acid composition were observed after the accelerated stability test. VOB 100 µg/mL reduced the healing time by increasing the total number of cells in the wounded area by 43.0±5.1% compared to the negative control group. VOB also suppressed the pro-inflammatory TNF-α and IL-6 cytokines, and NO and O 2 - production in lipopolysaccharide-stimulated macrophage cells. In conclusion, the VOB formulation contributed to the improvement of current therapeutic strategies for cutaneous applications in skin care.

Linoleic acid‐rich guava seed oil: Safety and bioactivity

“…Regarding the antiinflammatory effect, LA was found to be catalyzed by skin epidermis lipoxygenase into 13S‐hydroxyoctadecadienoic acid (13S‐HODE), which subsequently activated hydrolytic cleavage of the membrane inositol phospholipids into 13S‐HODE‐substituted diacylglycerol (Ziboh, Cho, Mani, & Xi, ). LA‐rich vegetable oil also increased the total number of cells in the wounded area and suppressed proinflammatory TNF‐α and IL‐6 cytokine production in lipopolysaccharide‐stimulated RAW 264.7 macrophage cells (Guidoni et al, ).…”

Determination of the fatty acids composition of refined sunflower oil and possibility of its changing during storage period

“…Sunflower oil is an auxiliary ingredient in compounding medicines. Most often it is part of ointments, creams [1] and suspensions. In many suspensions for the treatment of the oral mucosa, sunflower oil is the basis of the dosage forms, where it is combined with various pharmacologically active ingredients.…”

Liposomal Formulation of Snakehead Fish (Ophiocephalus striatus) Powder and Toxicity Study in Zebrafish (Danio rerio) Model

“…In addition, the major fatty acids are docosahexaenoic acid, eicosapentaenoic acid, linoleic acid, and arachidonic acid. [3][4][5] Inflammation is a non-explicit response of body tissues against destructive stimulus, injury, or infection to secure the body and promote the recovery process. Regarding this, a large group of disorders such as cancer, asthma, inflammatory bowel diseases, rheumatoid arthritis, allergies and sarcoidosis are originated from an inflammatory condition.…”

“…6 Some researchers have studied about the benefits of snakehead fish for reducing inflammation. Local people believed that snakehead fish reduces pain and use it mostly for treating mothers after delivery and any post-operative pain, 7 wound healing, 5,[8][9] anti-eczema, 10 and platelet aggregation 2 are some wellknown pharmacological properties of snakehead fish are demonstrated till now. Glycine is one of the amino acids which have the highest content in SFP.…”

“…Glycine is one of the amino acids which have the highest content in SFP. They have been shown many functions in the cell membrane for blood clothing 5 , wound healing and antinociceptive activities of snakehead fish. 11 Besides, four preliminary studies have reported that snakehead fish extract has potent antiinflammatory property [12][13][14][15][16] but to the best of our knowledge, no research was done to study the effect of snakehead fish aqueous extract in lipid base nanocarrier for inflammation and cancer.…”