Fatty acid profiles from the plasma membrane and detergent resistant membranes of two plant species

Carmona-Salazar, Laura; Hafidi, Mohammed El; Gutiérrez-Nájera, Nora; Noyola-Martínez, Liliana; González-Solís, Ariadna; Gavilanes‐Ruíz, Marina

doi:10.1016/j.phytochem.2014.10.017

Cited by 6 publications

(5 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Plant GIPCs exhibit a high content of VLCFAs that can be hydroxylated on carbon 2. The presence of VLCFAs in DIMs was recently observed in bean (Phaseolus vulgaris) and maize (Zea mays) DIMs (Carmona-Salazar et al, 2015). Here, the modeling approach suggests a strong and stable interdigitation of VLCFA and hVLCFA of GIPCs from the outer leaflet into the inner leaflet by six to seven carbon atoms (Fig.…”

Section: Saturated Vlcfas Of Gipcs Interdigitate the Two Leafletssupporting

confidence: 62%

Revisiting Plant Plasma Membrane Lipids in Tobacco: A Focus on Sphingolipids

et al. 2015

View full text Add to dashboard Cite

The lipid composition of plasma membrane (PM) and the corresponding detergent-insoluble membrane (DIM) fraction were analyzed with a specific focus on highly polar sphingolipids, so-called glycosyl inositol phosphorylceramides (GIPCs). Using tobacco (Nicotiana tabacum) 'Bright Yellow 2' cell suspension and leaves, evidence is provided that GIPCs represent up to 40 mol % of the PM lipids. Comparative analysis of DIMs with the PM showed an enrichment of 2-hydroxylated very-long-chain fatty acid-containing GIPCs and polyglycosylated GIPCs in the DIMs. Purified antibodies raised against these GIPCs were further used for immunogold-electron microscopy strategy, revealing the distribution of polyglycosylated GIPCs in domains of 35 6 7 nm in the plane of the PM. Biophysical studies also showed strong interactions between GIPCs and sterols and suggested a role for very-long-chain fatty acids in the interdigitation between the two PM-composing monolayers. The ins and outs of lipid asymmetry, raft formation, and interdigitation in plant membrane biology are finally discussed.Eukaryotic plasma membranes (PMs) are composed of three main classes of lipids, glycerolipids, sphingolipids, and sterols, which may account for up to 100,000 different molecular species (Yetukuri et al., 2008;Shevchenko and Simons, 2010). Overall, all glycerolipids share the same molecular moieties in plants, animals, and fungi. By contrast, sterols and sphingolipids are different and specific to each kingdom. For instance, the plant PM contains an important number of sterols, among which b-sitosterol, stigmasterol, and campesterol predominate (Furt et al., 2011). In addition to free sterols, phytosterols can be conjugated to form steryl glycosides (SG) and acyl steryl glycosides (ASG) that represent up to approximately 15% of the tobacco (Nicotiana tabacum) PM (Furt et al., 2010). As for sphingolipids, sphingomyelin, the major phosphosphingolipid in animals, which harbors a phosphocholine as a polar head, is not detected in plants. Glycosyl

show abstract

Section: Saturated Vlcfas Of Gipcs Interdigitate the Two Leafletssupporting

confidence: 62%

Revisiting Plant Plasma Membrane Lipids in Tobacco: A Focus on Sphingolipids

et al. 2015

View full text Add to dashboard Cite

show abstract

“…According to the fluidity results, an effect of the mycotoxin addition on the fatty acid content of the PM was explored, as it is known that membrane fatty acids composition is one of the major factors influencing membrane fluidity. We measured the levels of the more abundant fatty acids of the PM from maize embryos [26] when these were imbibed with or without FB1. Figure 4A shows that the total amount of fatty acids was unaffected in the PM from the embryos treated with FB1 as compared to the control embryos.…”

Section: Fb1 Addition Does Not Modify the Profile Of The Pm Main Fattmentioning

confidence: 99%

Sphingolipid Effects on the Plasma Membrane Produced by Addition of Fumonisin B1 to Maize Embryos

Gutiérrez-Nájera

Saucedo-García

Noyola-Martínez

et al. 2020

Plants

Self Cite

View full text Add to dashboard Cite

Fumonisin B1 is a mycotoxin produced by Fusarium verticillioides that modifies the membrane properties from animal cells and inhibits complex sphingolipids synthesis through the inhibition of ceramide synthase. The aim of this work was to determine the effect of Fumonisin B1 on the plant plasma membrane when the mycotoxin was added to germinating maize embryos. Fumonisin B1 addition to the embryos diminished plasma membrane fluidity, increased electrolyte leakage, caused a 7-fold increase of sphinganine and a small decrease in glucosylceramide in the plasma membrane, without affecting phytosphingosine levels or fatty acid composition. A 20%–30% inhibition of the plasma membrane H+-ATPase activity was observed when embryos were germinated in the presence of the mycotoxin. Such inhibition was only associated to the decrease in glucosylceramide and the addition of exogenous ceramide to the embryos relieved the inhibition of Fumonisin B1. These results indicate that exposure of the maize embryos for 24 h to Fumonisin B1 allowed the mycotoxin to target ceramide synthase at the endoplasmic reticulum, eliciting an imbalance of endogenous sphingolipids. The latter disrupted membrane properties and inhibited the plasma membrane H+-ATPase activity. Altogether, these results illustrate the mode of action of the pathogen and a plant defense strategy.

show abstract

“…Borner et al [4] and Mongrand et al [31] first quantified lipid class and fatty acid composition using DRM as microdomain-enriched fractions. In the same way, the DRM lipid compositions of maize embryos and bean leaves have been characterized [6,7]. Furt et al [12] reported that phosphatidylinositol-4,5-bisphosphate forms a cluster-like structure that is not influenced by sterol depletion, and that phosphoinositide metabolism-related enzyme activities in DRMs are higher than in the PM.…”

Section: Introductionmentioning

confidence: 99%

Lipid profiles of detergent resistant fractions of the plasma membrane in oat and rye in association with cold acclimation and freezing tolerance

et al. 2016

View full text Add to dashboard Cite

Cold acclimation (CA) results in alteration of the plasma membrane (PM) lipid composition in plants, which plays a crucial role in the acquisition of freezing tolerance via membrane stabilization. Recent studies have indicated that PM structure is consistent with the fluid mosaic model but is laterally non-homogenous and contains microdomains enriched in sterols, sphingolipids and specific proteins. In plant cells, the function of these microdomains in relation to CA and freezing tolerance is not yet fully understood. The present study aimed to investigate the lipid compositions of detergent resistant fractions of the PM (DRM) which are considered to represent microdomains. They were prepared from leaves of low-freezing tolerant oat and high-freezing tolerant rye. The DRMs contained higher proportions of sterols, sphingolipids and saturated phospholipids than the PM. In particular, one of the sterol lipid classes, acylated sterylglycoside, was the predominant sterol in oat DRM while rye DRM contained free sterol as the major sterol. Oat and rye showed different patterns (or changes) of sterols and 2-hydroxy fatty acids of sphingolipids of DRM lipids during CA. Taken together, these results suggest that CA-induced changes of lipid classes and molecular species in DRMs are associated with changes in the thermodynamic properties and physiological functions of microdomains during CA and hence, influence plant freezing tolerance.

show abstract

Fatty acid profiles from the plasma membrane and detergent resistant membranes of two plant species

Cited by 6 publications

References 63 publications

Revisiting Plant Plasma Membrane Lipids in Tobacco: A Focus on Sphingolipids

Revisiting Plant Plasma Membrane Lipids in Tobacco: A Focus on Sphingolipids

Sphingolipid Effects on the Plasma Membrane Produced by Addition of Fumonisin B1 to Maize Embryos

Lipid profiles of detergent resistant fractions of the plasma membrane in oat and rye in association with cold acclimation and freezing tolerance

Contact Info

Product

Resources

About