Fatty acids and triacylglycerols composition from Tunisian Acacia species seed oil

Youzbachi, Nouha; Trabelsi, H.; Elfalleh, Walid; Khaldi, Abdelhamid; Nasri, Nizar; Tlili, Nizar

doi:10.1016/j.arabjc.2015.08.020

Cited by 19 publications

(13 citation statements)

References 37 publications

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“…Lipid profile of the HM pools in different phases was evaluated embracing the mass/charge (m/z) ranging from 100 to 1200, in triplicate. For this, 50.0 mg of lipid from each HM pool extracted by Folch and creamatocrit methods and prepared according to Youzbachi et al 19 with modifications, were diluted in 950.0 μL of chloroform (Synth, São Paulo, Brazil). Subsequently, 5.0 μL of this solution was diluted with the addition of 1.0 mL of methanol/chloroform 9:1 (high-performance liquid chromatography (HPLC) grade, J.T.Baker ® , Radnor, USA).…”

Section: Sample Preparation Of Hm Pools Using Di-esi(+)-ms and Instrumentioning

confidence: 99%

Lipid Profile Determination by Direct Infusion ESI-MS and Fatty Acid Composition by GC-FID in Human Milk Pools by Folch and Creamatocrit Methods

Rydlewski¹,

Silva²,

Manin³

et al. 2019

J. Braz. Chem. Soc.

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Human milk (HM) is extremely important to the baby, containing a varied and balanced range of nutrients fundamental to the ideal development of the newborn. Among these nutrients, HM lipids provide 40-60% of the energy. In HM banks, the creamatocrit method is employed as a quick and solvent-free tool, however, since Folch methodology is considered reference for lipid extraction, this study compared the fatty acid (FA) composition and the lipid profile by gas chromatography with flame ionization detector (GC-FID) and direct infusion by electrospray ionization mass spectrometry (DI-ESI-MS) in pools of different HM phases, obtained from Folch and creamatocrit methods, in order to elucidate which method provides better information about HM lipids. Lipid compositon results by Folch were superior to the results obtained by creamatocrit in HM pools investigated. It means, the lipid profiles of all HM pools were more intense, and the number of FA identified by Folch was also higher in comparison to the creamatocrit method (21 and 6 FAs, respectively). GC-FID analysis in combination with principal component analysis (PCA) divided the methods, confirming a greater contribution of the Folch method. Therefore, Folch methodology revealed an effective lipid extraction in comparison to creamatocrit method.

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Section: Sample Preparation Of Hm Pools Using Di-esi(+)-ms and Instrumentioning

confidence: 99%

Lipid Profile Determination by Direct Infusion ESI-MS and Fatty Acid Composition by GC-FID in Human Milk Pools by Folch and Creamatocrit Methods

Rydlewski¹,

Silva²,

Manin³

et al. 2019

J. Braz. Chem. Soc.

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“…Vegetable oil, margarine, nuts and whole-grain products are important sources of omega-6 FAs (C18:2, (n-6)), while seafood, canola oil, soybean, flaxseed and walnuts are rich in omega-3 FAs (C18:3, (n-3). The consumption of omega-3 FAs is recommended to improve immunity, brain function and human health [8][9][10]. Generally, the seeds are good sources of protein and carbohydrates due to their high oil content [11].…”

Section: Introductionmentioning

confidence: 99%

Chemical, Nutritional and Antioxidant Characteristics of Different Food Seeds

et al. 2020

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The objective of this study was to determine the chemical composition of five different food seeds (sunflower, poppy, hemp, flax and sesame) regarding fatty acid, mineral (Fe, Cu, Zn, Na, Mg, K, Ca, Al) and protein content. In addition, the total antioxidant capacity of the seeds was evaluated using the photochemiluminescent assay. The food seeds were subjected to lipid extraction and converted into fatty acid methyl esters before the gas chromatography analysis. In all food seeds, the saturated (SFAs), monounsaturated (MUFAs) and polyunsaturated fatty acids (PUFAs) were identified, respectively. PUFAs were the most abundant fatty acids (61.2% ± 0.07% and 84.8% ± 0.08% of total fatty acids), with the highest content in flax and hemp seed oil. Also, high amounts of omega-3 from PUFAs were determined in flax and hempseed oil. Based on the obtained results the sunflower, sesame and poppy seeds are good sources of omega-6, while flax and hemp seeds are good sources of omega-3. All samples are rich in minerals (Na, K, Ca, Mg) and have more than 20% protein content.

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“…In addition, soybean oil was assessed to compare distinctive chemical profiles with those obtained for omega-3 supplements and to detect possible adulterations. The lipid materials were prepared based on Youzbachi et al 20 14 Therefore, ammonium formate was added to samples in order to form adducts and consequently prevailing the ionization via [TAG + NH 4 ] + so that the MS fingerprint reproducibility was not affected.…”

Section: Sample Preparation For Ms Fingerprinting Esi(+)-msmentioning

confidence: 99%

Quality Assessment of Omega-3 Supplements Available in the Brazilian Market

Galuch¹,

Carbonera²,

Magon³

et al. 2017

J. Braz. Chem. Soc.

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Omega-3 fish oil supplements are widely consumed as source of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, presenting beneficial effects on human health. This study aimed to evaluate fifteen brands of omega-3 fish oil supplements available in Brazilian market in order to estimate the Brazilian reality regarding those supplements. Twelve fatty acids were quantified by gas chromatography with a flame ionization detector (GC-FID), and lipid profile were obtained via mass spectrometry fingerprinting using direct electrospray ionization mass spectrometry (ESI-MS) to assess the form in which fatty acids are present as well as the possible fraud existence. Among all analyzed samples, thirteen brands were revealed as EPA and DHA sources (90.2-440.3 and 77.8-302.3 mg g -1 lipid, respectively) in triacylglycerols (TAG) or ethyl esters (EE) form. However, two brands were discovered with addition of large amounts of soybean oil, leading the final consumer to ingest this low-cost oil believing that they are consuming adequate doses of EPA and DHA.

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Fatty acids and triacylglycerols composition from Tunisian Acacia species seed oil

Cited by 19 publications

References 37 publications

Lipid Profile Determination by Direct Infusion ESI-MS and Fatty Acid Composition by GC-FID in Human Milk Pools by Folch and Creamatocrit Methods

Lipid Profile Determination by Direct Infusion ESI-MS and Fatty Acid Composition by GC-FID in Human Milk Pools by Folch and Creamatocrit Methods

Chemical, Nutritional and Antioxidant Characteristics of Different Food Seeds

Quality Assessment of Omega-3 Supplements Available in the Brazilian Market

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