Fatty acids and TxA2 generation, in the absence of platelet-COX-1 activity

Abstract

Background and aims Omega-3 fatty acids suppress Thromboxane A2 (TxA2) generation via mechanisms independent to that of aspirin therapy. We sought to evaluate whether baseline omega-3 fatty acid levels influence arachidonic acid proven platelet-cyclooxygenase-1 (COX-1) independent TxA2 generation (TxA2 generation despite adequate aspirin use). Methods and results Subjects with acute myocardial infarction, stable CVD or at high risk for CVD, on adequate aspirin therapy were included in this study. Adequate aspirin action was defined as complete inhibition of platelet-COX-1 activity as assessed by <10% change in light transmission aggregometry to ≥1 mmol/L arachidonic acid. TxA2 production was measured via liquid chromatography–tandem mass spectrometry for the stable TxA2 metabolite 11-dehydro-thromboxane B2 (UTxB2) in urine. The relationship between baseline fatty acids, demographics and UTxB2 were evaluated. Baseline omega-3 fatty acid levels were not associated with UTxB2 concentration. However, smoking was associated with UTxB2 in this study. Conclusion Baseline omega-3 fatty acid levels do not influence TxA2 generation inpatients with or at high risk for CVD receiving adequate aspirin therapy. The association of smoking and TxA2 generation, in the absence of platelet COX-1 activity, among aspirin treated patients warrants further study.

Micronutrients, Phytochemicals and Mediterranean Diet: A Potential Protective Role against COVID-19 through Modulation of PAF Actions and Metabolism

“…Omega-3 fatty acids also exert antithrombotic effects by various mechanisms including a reduction in thromboxane synthesis [ 99 ] and PAF [ 100 , 101 ]. Omega-3 fatty acids are incorporated in the cell membrane and may regulate the activity of PLA 2 and thus lyso-PAF production, which is a prodrome molecule for PAF production [ 102 ].…”

Influence of smoking and smoking cessation on levels of urinary 11-dehydro thromboxane B2

“…Of the 21 studies, ten were included in the meta-analyses [ 10 , 14 , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] ]. The other 11 studies were not included due to having incomplete information [ 26 , 27 ], results given in different units [ [28] , [29] , [30] , [31] ], study results had been published in another study [ 1 , 32 ], measurements were reported from plasma concentrations [ 13 ] or included diseased populations [ 33 , 34 ]. Characteristics of all studies included in the analyses can be found in Table 1 .…”

Potential Cardiovascular and Metabolic Beneficial Effects of ω-3 PUFA in Male Obesity Secondary Hypogonadism Syndrome

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