Fatty acids, phenolic compounds and antioxidant capacity of the seeds from nine grape cultivars (Vitis viniferaL.)

Abstract

Seeds are one of the main parts of the grapes, and they contain important constituents such as polyphenols, lipids, proteins and carbohydrates. In this study, oil contents, fatty acid composition, total phenolic content, total flavonoid content, monomeric flavan-3-ols and antioxidant capacities of nine grape seeds from nine cultivars were investigated. The correlations between the analyzed parameters were also examined. The oil contents of seeds ranged from 4.96 to 13.35%. Linoleic acid was the predominant fatty acid in the seeds, and it was followed by oleic acid, palmitic acid, stearic acid, linolenic acid, 11-eicosenoic acid and arachidic acid. Total phenolic and total flavonoid contents were 646.50-1120.78 mg GAE/g and 336.69-589.85 mg CE/g, respectively. ‘Italia’ seeds had the highest antioxidant capacity for DPPH and CUPRAC assays while ‘Cabernet Sauvignon’ seeds had the lowest one. The (-)-epigallocatechin, (+)-catechin, (-)-epigallocatechin gallate, (-)-epicatechin and (-)-epicatechin gallate contents in the seeds varied from 1.23 to 6.53 mg/g, 11.61 to 80.20 mg/g, 0.21 to 0.51 mg/g, 2.78 to 48.26 mg/g and 0.08 to 1.50 mg/g, respectively. The (+)-catechin and (-)-epicatechin were the major flavan-3-ol compounds in the grape seeds. Significant correlations were found between total phenolic content, total flavonoid, RSA (DPPH Radical scavenging activity), CUPRAC (Cupric reducing antioxidant capacity) and flavan-3-ols. Consequently, the current study findings support previous information that grape seeds are an important source of antioxidants as well as unsaturated fatty acids, which have positive effects on human health.