Fc‐conjugated C‐type lectin receptors: Tools for understanding host–pathogen interactions

Abstract

For ease of purification and a number of other beneficial factors, which will be discussed below, the C-type lectin receptor's (CLR's) ligand-binding domain is generally fused to the hinge region of the human immunoglobulin G1 (IgG1) Fc portion containing the constant heavy (CH) 1 and CH2 domains (Figure 1), which depending on the final application, can have modifications altering the Fc proteins' in vivo effector functions, for example, reduced activation of complement C1q (P331S), decreased binding to mammalian cell

Vector and Host C-Type Lectin Receptor (CLR)–Fc Fusion Proteins as a Cross-Species Comparative Approach to Screen for CLR–Rift Valley Fever Virus Interactions

“…While soluble CLR–hFc fusion proteins are useful to screen for pathogen–CLR interactions, the mode of presentation of the CRD may markedly affect ligand recognition [ 19 , 34 , 35 , 46 , 59 ]. By using a library of dimeric CLR–hFc fusion proteins with the CRD N-terminally fused to the Fc fragment of human IgG1, we may miss RVFV interactions with CLRs for which a multimeric presentation is crucial, thereby leading to false-negative results.…”

Enabling the formation of native mAb, Fab′ and Fc-conjugates using a bis-disulfide bridging reagent to achieve tunable payload-to-antibody ratios (PARs)

“…8a Only a few Fc-conjugates have recently been reported on literature thus far. They were generated either via recombinant methods, 54,55 or aer enzymatic modication of recombinant Fc. [56][57][58] To the best of our knowledge, no site-selective chemical modication of a non-engineered Fc fragment has been described as yet.…”

A Critical Role for CARD9 in Intestinal Microbiota Modulation and Colorectal Malignancies

“…C-type lectin receptors (CLRs), known as membraneassociated receptors, are critical for the detection of pathogenic and commensal fungal signals [31]. CARD9 functions as an adaptor molecule downstream of the CLRs.…”