Fear conditioning suppresses large-conductance calcium-activated potassium channels in lateral amygdala neurons

Small conductance calcium activated potassium (SK) channel dependent and independent effects of riluzole on neuropathic pain-related amygdala activity and behaviors in rats

“…Previous studies correlating drug effects in brain slices with drug effects using microdialysis in the intact rat showed that a 100-fold greater drug concentration in the microdialysis fiber than the desired target concentration in the tissue was needed due to the concentration gradient across the microdialysis membrane and diffusion into the brain tissue (Fu and Neugebauer, 2008; Ji et al, 2013; Ji et al, 2010). The concentrations for drug application by microdialysis (riluzole (Cao et al, 2002); apamin (Faber and Sah, 2007); ChTx (Sun et al, 2015)) and for drug application in brain slices (AP5 (Bird et al, 2005)) were chosen based on previously published electrophysiological studies.…”

Homer1a-dependent recovery from depression-like behavior by photic stimulation in mice

“…Experiments and analysis were done as described [13,14,25,26]. Briefly, mice used for the behavioral test were sacrificed by decapitation under ether anesthesia.…”

“…On the same day as behavioral tests were completed, 3 mice from each experimental group were sacrificed and immediately frozen for later qRT-PCR experiments, which were done as described [13,25]. Cerebral cortical tissue was rapidly dissected out, placed into liquid nitrogen, and then stored at −80°C until used for mRNA extraction with an RNeasy lipid tissue kit (QIAGEN-Japan K.K., Tokyo).…”

Esco2 and Cohesin Regulate CRL4 Ubiquitin Ligaseddb1Expression and Thalidomide Teratogenicity

“…In the latter case, the sea change in transforming RBS from one of mitotic failure to that of transcriptional dysregulation has profound implications for treatment. For instance, ddb1 and crbn mutations result in elevated BKCa high conductance channel trafficking to the plasma membrane, increased ion conductivity, high neuronal excitation, and seizures (27)(28)(29). Paxilline, a BKCa channel blocker, reduces the incidence and severity of seizures (28).…”