Feasibility of Human Platelet Lysate as an Alternative to Foetal Bovine Serum for In Vitro Expansion of Chondrocytes

Liau, Ling Ling; Hassan, Muhammad Najib Fathi bin; Tang, Yee Loong; Ng, Min Hwei; Law, Jia Xian

doi:10.3390/ijms22031269

Cited by 23 publications

(19 citation statements)

References 104 publications

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“…Cartilage is an avascular and alymphatic tissue. 111 Thus, unlike in other tissue injuries, exosomes are not suitable for infusing intravenously for treating cartilage defects. In fact, all of the included studies administered exosomes directly to the affected joint, as intra-articular administration increases bioavailability and reduces off-target effects, thereby improving the delivery of exosomes to the cartilage and decreasing the dose of exosomes needed.…”

Section: Resultsmentioning

confidence: 99%

Potential of Exosomes as Cell-Free Therapy in Articular Cartilage Regeneration: A Review

Ng¹,

Chai²,

Foo³

et al. 2021

IJN

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Treatment of cartilage defects such as osteoarthritis (OA) and osteochondral defect (OCD) remains a huge clinical challenge in orthopedics. OA is one of the most common chronic health conditions and is mainly characterized by the degeneration of articular cartilage, shown in the limited capacity for intrinsic repair. OCD refers to the focal defects affecting cartilage and the underlying bone. The current OA and OCD management modalities focus on symptom control and on improving joint functionality and the patient’s quality of life. Cell-based therapy has been evaluated for managing OA and OCD, and its chondroprotective efficacy is recognized mainly through paracrine action. Hence, there is growing interest in exploiting extracellular vesicles to induce cartilage regeneration. In this review, we explore the in vivo evidence of exosomes on cartilage regeneration. A total of 29 in vivo studies from the PubMed and Scopus databases were identified and analyzed. The studies reported promising results in terms of in vivo exosome delivery and uptake; improved cartilage morphological, histological, and biochemical outcomes; enhanced subchondral bone regeneration; and improved pain behavior following exosome treatment. In addition, exosome therapy is safe, as the included studies documented no significant complications. Modifying exosomal cargos further increased the cartilage and subchondral bone regeneration capacity of exosomes. We conclude that exosome administration is a potent cell-free therapy for alleviating OA and OCD. However, additional studies are needed to confirm the therapeutic potential of exosomes and to identify the standard protocol for exosome-based therapy in OA and OCD management.

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Section: Resultsmentioning

confidence: 99%

Potential of Exosomes as Cell-Free Therapy in Articular Cartilage Regeneration: A Review

Ng¹,

Chai²,

Foo³

et al. 2021

IJN

Self Cite

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“…Recent studies have confirmed that HAS enhances the proliferation of chondrocytes and PRP boosts the proliferation and redifferentiation of dedifferentiated chondrocytes ( Jeyakumar et al, 2017 ; Jeyakumar et al, 2019 ). The current research on PL supports its application to promote the expansion of chondrocytes; however, there are doubts regarding the regulation of chondrocyte redifferentiation ( Rikkers et al, 2020 ; Liau et al, 2021 ). Recent studies of LMWF5A have shown its potential in inducing fibroblast-like chondrocytes to redifferentiate into functional chondrocytes ( Hausburg et al, 2018 ).…”

Section: Extracellular Microenvironmentmentioning

confidence: 99%

Strategies to Modulate the Redifferentiation of Chondrocytes

Zhang

et al. 2021

Front. Bioeng. Biotechnol.

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Because of the low self-healing capacity of articular cartilage, cartilage injuries and degenerations triggered by various diseases are almost irreversible. Previous studies have suggested that human chondrocytes cultured in vitro tend to dedifferentiate during the cell-amplification phase and lose the physiological properties and functions of the cartilage itself, which is currently a critical limitation in the cultivation of cartilage for tissue engineering. Recently, numerous studies have focused on the modulation of chondrocyte redifferentiation. Researchers discovered the effect of various conditions (extracellular environment, cell sources, growth factors and redifferentiation inducers, and gene silencing and overexpression) on the redifferentiation of chondrocytes during the in vitro expansion of cells, and obtained cartilage tissue cultured in vitro that exhibited physiological characteristics and functions that were similar to those of human cartilage tissue. Encouragingly, several studies reported positive results regarding the modulation of the redifferentiation of chondrocytes in specific conditions. Here, the various factors and conditions that modulate the redifferentiation of chondrocytes, as well as their limitations and potential applications and challenges are reviewed. We expect to inspire research in the field of cartilage repair toward the future treatment of arthropathy.

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“…This term is mainly to describe the various types of cells (e.g. chondrocytes and MSCs [ 23 , 163 ]), which can develop the property of chondrocytes to form cartilage. This term may also be used to describe tissue engineered constructs to form cartilage.…”

Section: Bioactive Peptide To Promote Chondrogenesismentioning

confidence: 99%

Chondroinductive/chondroconductive peptides and their-functionalized biomaterials for cartilage tissue engineering

Zhu

Zhong

Cao

et al. 2022

Bioactive Materials

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The repair of articular cartilage defects is still challenging in the fields of orthopedics and maxillofacial surgery due to the avascular structure of articular cartilage and the limited regenerative capacity of mature chondrocytes. To provide viable treatment options, tremendous efforts have been made to develop various chondrogenically-functionalized biomaterials for cartilage tissue engineering. Peptides that are derived from and mimic the functions of chondroconductive cartilage extracellular matrix and chondroinductive growth factors, represent a unique group of bioactive agents for chondrogenic functionalization. Since they can be chemically synthesized, peptides bear better reproducibility, more stable efficacy, higher modifiability and yielding efficiency in comparison with naturally derived biomaterials and recombinant growth factors. In this review, we summarize the current knowledge in the designs of the chondroinductive/chondroconductive peptides, the underlying molecular mechanisms and their-functionalized biomaterials for cartilage tissue engineering. We also systematically compare their in-vitro and in-vivo efficacies in inducing chondrogenesis. Our vision is to stimulate the development of novel peptides and their-functionalized biomaterials for cartilage tissue engineering.

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Feasibility of Human Platelet Lysate as an Alternative to Foetal Bovine Serum for In Vitro Expansion of Chondrocytes

Cited by 23 publications

References 104 publications

Potential of Exosomes as Cell-Free Therapy in Articular Cartilage Regeneration: A Review

Potential of Exosomes as Cell-Free Therapy in Articular Cartilage Regeneration: A Review

Strategies to Modulate the Redifferentiation of Chondrocytes

Chondroinductive/chondroconductive peptides and their-functionalized biomaterials for cartilage tissue engineering

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