Feasibility of synchrotron radiation computed tomography on rats bearing glioma after iodine or gadolinium injection

Abstract

The purpose of this work was to demonstrate the feasibility of a new imaging technique called synchrotron radiation computed tomography (SRCT). This technique leads to a direct assessment of the in vivo concentration of an iodine- or gadolinium-labeled compound. Rats bearing C6 glioma were imaged by MRI prior to the SRCT experiment. The SRCT experiments were performed after a 1.3 g I/kg (n = 5) or a 0.4 g Gd/kg (n = 5) injection. Finally, brains were sampled for histology. The SRCT images exhibited contrast enhancement at the tumor location. Ten minutes after injection, iodine and gadolinium tissular concentrations were equal to 0.80 ( +/- 0.40) mg/cm3 and 0.50 ( +/- 0.10) mg/cm3, respectively in the peripheral area of the tumor (respective background value: 0.20 +/- 0.02 to 0.10 +/- 0.01). Correlation to MRI and histology revealed that the contrast uptake occurred in the most vascularized area of the tumor. The present study summarizes the feasibility of in vivo SRCT to obtain quantitative information about iodine and gadolinium-labeled compounds. Beyond brain tumor pathology, the SRCT appears as a complementary approach to MRI and CT, for studying iodine- and gadolinium-labeled compounds by the direct achievement of the tissular concentration value in the tissue.

γ-H2AX as a Marker for Dose Deposition in the Brain of Wistar Rats after Synchrotron Microbeam Radiation

“…C6 gliomas were originally produced as a result of exposing Wistar-furth rats to N-nitrosomethylurea, and then isolated and grown as a cell culture [ 59 ]. This tumour model has been used in multiple studies involving conventional radiotherapy [ 60 – 62 ] and synchrotron radiation [ 63 – 65 ].…”

Prolonged Blood-Brain Barrier Injury Occurs After Experimental Intracerebral Hemorrhage and Is Not Acutely Associated with Additional Bleeding

“…This method allowed for detailed spatial analysis of BBB damage, while allowing us to directly relate BBB permeability to PHZ ion dyshomeostasis. We are the first to use this approach in rodent stroke models, although others have measured Gd with synchrotron techniques to assess other pathologies [ 29 , 30 ]. XFI was compared to ICP-MS.…”

K-edge Subtraction Computed Tomography with a Compact Synchrotron X-ray Source

“…Monochromatic imaging allows elemental decomposition at a higher accuracy than state-of-the-art clinical imaging 13 . In addition, the absolute concentration of the contrast agent 28–30 can be determined in KES CT as well as the electron density of the material after performing a dual-energy approach 31–33 . In most KES CT synchrotron studies the clinical applicability was limited by the dose applied to the specimen, with dose levels between 7 mGy 33 and 9 Gy (skin entry dose) 19 .…”