Feasibility study of a biocompatible pneumatic dispensing system using mouse 3T3-J2 fibroblasts

Abstract

This paper presents results for dispensing living cells using a pneumatic dispensing system to verify the feasibility of using this system to fabricate biomaterials. Living cells (i.e., mouse 3T3-J2 fibroblast) were dispensed with different dispensing pressures in order to evaluate the effect of dispensing process on cell viability and proliferation. Based on the results of a live-dead assay, more than 80% of cell viability has been confirmed which was reasonably similar to that in the control group. Furthermore, measurement of cell metabolic activity after dispensing confirmed that the dispensed cell proliferated at a rate comparable to that of the control group. These results demonstrate that the pneumatic dispensing system is a promising tool for fabrication of biomaterials.

A 3D-printed pneumatic dispenser with monitoring droplet ejection

“…In this study, we introduced a pneumatic dispenser [10][11][12] that can monitor the droplet ejection status in real-time using a capacitive-type sensor embedded on a flexible membrane using pneumatic pressure deformation. The difference between the membrane deformation under normal and abnormal operating conditions was…”