Feather keratin hydrolysis by a Vibrio sp. strain kr2

Sangali, Sidnei; Brandelli, Adriano

doi:10.1046/j.1365-2672.2000.01173.x

Cited by 206 publications

(192 citation statements)

References 32 publications

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“…Tryptic soy agar (TSA, Difco, New Jersey) is a generalized medium, whereas feather meal agar (FMA; 15 gL )1 feather meal, 0. [25]. Both media types contained 100 lL/mL of cycloheximide to inhibit fungal growth [29], and all plates were incubated at room temperature (28°C) for 1 week.…”

Section: Cultures-independent Methodsmentioning

confidence: 99%

Microbial Diversity of Wild Bird Feathers Revealed throughCulture-Based and Culture-Independent Techniques

et al. 2005

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Despite recent interest in the interactions between birds and environmental microbes, the identities of the bacteria that inhabit the feathers of wild birds remain largely unknown. We used culture-based and culture-independent surveys of the feathers of eastern bluebirds (Sialis sialis) to examine bacterial flora. When used to analyze feathers taken from the same birds, the two survey techniques produced different results. Species of the poorly defined genus Pseudomonas were most common in the molecular survey, whereas species of the genus Bacillus were predominant in the culture-based survey. This difference may have been caused by biases in both the culture and polymerase chain reaction techniques that we used. The pooled results from both techniques indicate that the overall community is diverse and composed largely of members of the Firmicutes and b-and c-subdivisions of the Proteobacteria. For the most part, bacterial sequences isolated from birds were closely related to sequences of soil-borne and water-borne bacteria in the GenBank database, suggesting that birds may have acquired many of these bacteria from the environment. However, the metabolic properties and optimal growth requirements of several isolates suggest that some of the bacteria may have a specialized association with feathers.

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Section: Cultures-independent Methodsmentioning

confidence: 99%

Microbial Diversity of Wild Bird Feathers Revealed throughCulture-Based and Culture-Independent Techniques

et al. 2005

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“…In study of Sangali and Brandeli, the optimal growth of keratinolytic Vibrio sp. strain KR2 was observed at pH=7 and 30 ˚C (16). The isolated bacteria could grow at 28, 30, 32, 34 and 36℃ but the optimal growth temperature after 96 hours for Bacillus and Enterobacter was 32 ℃ and 34℃, respectively.…”

Section: Discussionmentioning

confidence: 96%

“…A study by Sangali and Brandli reported the keratinolytic activity of Vibrio sp. strain KR2 in a medium containing raw feather (16 (17). On the contrary, some studies relied on the biochemical detection and isolation of keratinolytic bacteria.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Molecular Identification of Keratinase-Producing Bacteria from the Sludge of Qeshm Island

2018

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Background and Objectives: Keratinase is an enzyme commonly used in the production of detergents, cosmetics, drugs, leather, and other industries. Considering the high cost of traditional methods for decomposition of feather, hair, hooves, nails, and wool that contain high levels of keratin, their biodegradation with keratinase-producing bacteria can be a valuable solution. The present study aimed for isolation and molecular identification of keratinase-producing bacteria in Qeshm Island and Peyposht village in Iran.Methods: Water and sludge samples from the Qeshm Island and Peyposht village were collected. The bacteria isolates were screened for keratinase production using the Lowry method. Effect of pH and temperature was assessed on the production of keratinase and on the growth of the isolates. Colony-polymerase chain reaction was used for molecular identification of the isolates.Results: Bacillus berevis and Enterobacter cloacae were isolated in this study. Keratinase production in B. berevis was highest at pH 7.5 and 35 °C. In addition, the highest level of enzyme production by E. cloacae was observed at pH 7 and 37 °C.Conclusion: It seems that the bacterial strains isolated from sludge in the study area have relatively favorable keratinase production capacity.

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“…Studies on Gram-negative bacteria are relatively scarce and keratinase activity has been described for Vibrio sp. (Sangali and Brandelli, 2000) Chryseobacterium sp. (Riffel et al, 2003) Xanthomonas sp.…”

Section: Discussionmentioning

confidence: 99%

“…Bacterial keratinases are of particular interest because of their action on the degradation of the insoluble keratin substrates and generally on a broad range of protein substrates (Lin et al, 1996). Many studies monitored the production of keratinase by Bacillus licheniformis PWD-1, Fervidobacterium, Chryseobacterium sp., Streptomyces, (Sangali and Brandelli, 2000;Langeveld et al, 2003;Riffel et al, 2003). Chryseobacterium sp., strain kr6 was isolated from waste of a poultry industry and was capable to completely degrade chicken feathers.…”

Section: Introductionmentioning

confidence: 99%

Biochemical and Molecular Characterization of a New Local Keratinase Producing Pseudomomanas sp., MS21

Tork¹,

Aly²,

Nawar³

2009

Asian J. of Biotechnology

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This study aimed to isolate and identify a new local bacterial strain, able to completely degrade keratin-rich wastes into soluble and useful materials which can be used in many proposes. Bacterial keratinases are of particular interest because of their action on insoluble keratin substrates and generally on a broad range of protein substrates. These enzymes have been studied for de-hairing processes in the leather industry and hydrolysis of feather and keratin. Samples from poultry industry wastes, soil, water, fodder and feather were collected from different places in Jeddah, Saudi Arabia. Each sample was plated on feather meal agar plates containing 5 g LG feather as the sole carbon and nitrogen source and 1 the obtained colonies were selected, purified and their growth were detected on skimmed milk agar and feather meal broth media. The well grown isolates on feather meal agar which producing the largest clearing zone on skimmed milk plate were selected for keratinase assays. Out of 23 bacterial isolates, 7 isolates were selected. The best keratinase producing bacterium kera MS21 was selected and identified based on morphological, physiological and some biochemical characteristics. It was recorded as a species belonging to the genus Pseudomonas and identified as Pseudomonas sp. The results of identification were confirmed by 16S rDNA studies. Precipitation and purification of the keratinase enzyme in addition to factors affecting enzyme activity were studied. The enzyme molecular weight was determined to be of 30 KDa using sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis. The optimum temperature and pH were determined to be 37°C and pH 8.0, respectively. The effect of some proteases inhibitors and activators were also studied.

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Feather keratin hydrolysis by a Vibrio sp. strain kr2

Cited by 206 publications

References 32 publications

Microbial Diversity of Wild Bird Feathers Revealed throughCulture-Based and Culture-Independent Techniques

Microbial Diversity of Wild Bird Feathers Revealed throughCulture-Based and Culture-Independent Techniques

Isolation and Molecular Identification of Keratinase-Producing Bacteria from the Sludge of Qeshm Island

Biochemical and Molecular Characterization of a New Local Keratinase Producing Pseudomomanas sp., MS21

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