Features of canna regeneration in vitro and plantlets adaptation in vivo

Clonal micropropagation of essential oil rose cultivars and breeding samples at long-term subcultivationin vitro

“…The formation of several shoots at the stage of explant introduction in vitro was noted in many works devoted to the micropropagation of essential oil roses [2,[8][9][10]12]. However, some authors noted that explants exude dark colored compounds into the culture medium (phenols, pigments) that are released from the cut ends of the explants [14].…”

“…These publications mostly covered issues of R. damascena propagation in vitro [2,[5][6][7][8]. As explants when introduced into an aseptic culture, axillary or apical buds and meristems [5,[8][9][10], stem segments with a node [7,9,[11][12] were usually used. There is evidence of the use of direct shoot regeneration from leaf, stem and petiole for R. damascena micropropagation [1].…”

“…The compositions of culture media recommended by different authors for the multiplication stage are very diverse [1-2, 9, 11, 13]. As cytokinin, many authors recommend the introduction of 6-benzylaminopurine (BAP) into the culture medium composition [6,[9][10][11]14]. Thus, some studies have shown the efficiency of relatively high concentrations of this growth regulator -3.0 mg/l [11][12], 4.0 mg /l [9], 6.0 mg/l [7].…”

“…In addition to the influence of the culture medium composition, in some studies the effect of other exogenous or endogenous factors on the development of rose explants during micropropagation was analyzed. An important role in this process of the genotype [5-6, 8, 15], explant type [1][2], explant size [7], explant isolation season [5,10] was noted for scented roses. So, in the work of Allahverdi Mamaghani et al it was noted that appropriate seasons for introduction in vitro were summer and autumn [5].…”

“…So, in the work of Allahverdi Mamaghani et al it was noted that appropriate seasons for introduction in vitro were summer and autumn [5]. For different regions of the Crimea, the best time for introducing explants into the culture was February-March [10] or August [18]. Some papers have noted the influence of light source on rose micropropagation [1].…”