Features of clonal micropropagation of Silene cretacea (Caryophyllaceae) in in vitro culture

Kritskaya, T. A.; Кашин, А. С.; Spivak, V. A.; Firstov, Viktor Egorovich

doi:10.1134/s1062360416060023

Cited by 7 publications

(12 citation statements)

References 10 publications

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“…According to the protocol proposed by Hanus-Fajerska (2011), the use of 0.1 mg l −1 NAA and 0.5 mg l −1 BA for the establishment of S. vulgaris calamine ecotype shoot culture brought a positive effect. Likewise, Corduk et al (2018) reported that the same auxin and cytokinin type was suitable for in vitro propagation of S. bolanthoides, while Kritskaya et al (2016) showed that combination with kinetin and gibberellic acid was the most effective for micropropagating shoots of S. cretacea. In our study, we also tested the medium with calcium gluconate to increase calcium supplementation, an excess content of which was noted in wastes obtained from Zn-Pb ores exploitation and processing .…”

Section: Optimization Of Shoot Culture Protocolmentioning

confidence: 99%

Structural, physiological and genetic diversification of Silene vulgaris ecotypes from heavy metal-contaminated areas and their synchronous in vitro cultivation

Muszyńska

Labudda

Różańska

et al. 2019

Planta

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Main conclusion Results provide significant comparison of leaf anatomy, pigment content, antioxidant response and phenolic profile between individuals from miscellaneous populations and describe unified cultivation protocols for further research on stress biology.The plant communities growing on heavy metal-polluted areas have attracted considerable attention due to their unique ability to tolerate enormous amounts of toxic ions. Three ecotypes of Silene vulgaris representing calamine (CAL), serpentine (SER) and non-metallicolous (NM) populations were evaluated to reveal specific adaptation traits to harsh environment. CAL leaves presented a distinct anatomical pattern compared to leaves of SER and NM plants, pointing to their xeromorphic adaptation. These differences were accompanied by divergent accumulation and composition of photosynthetic pigments as well as antioxidant enzyme activity. In CAL ecotype, the mechanism of reactive oxygen species scavenging is based on the joint action of superoxide dismutase and catalase, but in SER ecotype on superoxide dismutase and guaiacol-type peroxidase. On the contrary, the concentration of phenylpropanoids and flavonols in the ecotypes was unchanged, implying the existence of similar pathways of their synthesis/degradation functioning in CAL and SER populations. The tested specimens showed genetic variation (atpA/MspI marker). Based on diversification of S. vulgaris populations, we focused on the elaboration of similar in vitro conditions for synchronous cultivation of various ecotypes. The most balanced shoot culture growth was obtained on MS medium containing 0.1 mg l −1 NAA and 0.25 mg l −1 BA, while the most abundant callogenesis was observed on MS medium enriched with 0.5 mg l −1 NAA and 5.0 mg l −1 BA. For the first time, unified in vitro protocols were described for metallophytes providing the opportunity to conduct basic and applied research on stress biology and tolerance mechanisms under freely controlled conditions.Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Section: Optimization Of Shoot Culture Protocolmentioning

confidence: 99%

Structural, physiological and genetic diversification of Silene vulgaris ecotypes from heavy metal-contaminated areas and their synchronous in vitro cultivation

Muszyńska

Labudda

Różańska

et al. 2019

Planta

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“…Kinetin, together with the auxin (NAA), participates in the organogenesis process in plants. This is supported by Kritskaya et al [8], who found that Kin was effective in the micropropagation of Silene cretacea. Also, it has been reported on other Silene species that cytokinin and auxin are required in combination in tissue culture media and are the best for the initial shoot induction phase [10].…”

Section: Discussionmentioning

confidence: 56%

“…The main property of GA 3 is the stimulation of cell division and elongation and the presence of auxin (NAA) in the medium enhances the action of GA 3 [8,25]. This was confirmed by Kritskaya et al [8], who successfully micropropagated Silene cretacea by using GA 3 and auxin in the medium in a combination with cytokinin. Don Palmer and Keller [26] reported that explant source is one of the important factors for the successful establishment of in vitro culture.…”

Section: Discussionmentioning

confidence: 84%

In vitro propagation and DNA barcode analysis of the endangered Silene schimperiana in Saint Katherine protectorate

Ghareb

Ibrahim

Hegazi

2020

Journal of Genetic Engineering and Biotechnology

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Background: Anthropogenic activity, climate change, pollution, and exploitation of natural resources are some reasons that cause threatening of plant diversity. Silene schimperiana is an endangered plant species in Egypt and is endemic to the high mountain of Saint Katherine Protected Area in southern Sinai. The purpose of the study was the ex situ conservation of Silene schimperiana through in vitro propagation and DNA barcode analysis. Results: To develop an efficient ex situ conservation program of the plant, in vitro propagation protocol has been achieved from shoot tip and stem nodal segment explants of in vitro germinated seedlings. Explants were established in vitro on Murashige and Skoog (MS) medium supplemented with 2.89 μM gibberellic acid (GA 3) , 1.08 μM α-naphthaleneacetic acid (NAA), and 1.16 μM kinetin (Kin). The highest number of axillary shoots (9.27) was obtained when they were transferred to MS medium supplemented with 4.48 μM 6-benzyl adenine (BA). Hundred percent of multiple axillary shoots were rooted on quarter-strength MS medium supplemented with 4.92 μM indole-3-butyric acid (IBA) and 10.75 μM NAA. Rooted plants were transferred to pots containing a soil-peat mixture (1: 2 v/v) and successfully acclimatized in the greenhouse. Plant identification is a crucial aspect to understand and conserve plant diversity from extinction. DNA barcode analysis of Silene schimperiana was carried out using two chloroplast DNA markers (cpDNA): 1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) and RNA polymerase subunit (rpoC1) and a nuclear ribosome DNA marker (ncDNA), internal transcribed spacer (ITS). Phylogenetic analysis revealed a successful identification of Silene schimperiana on the species and genus levels and supported the inclusion of Silene schimperiana in genus Silene. Conclusions: In this study, a relevant in vitro propagation method was established to facilitate the recovery of Silene schimperiana, in addition to DNA barcoding of the plant as a tool for effective management and conservation of plant genetic resources.

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“…These shoots propagated in a large number in the propagation stage. Similarly, it has been reported on various Silene species that PGRs supplemented to the culture medium improve the number of shoots and shoot length [23][24][25].…”

Section: Discussionmentioning

confidence: 85%

“…In recent years, many studies have been devoted to in vitro cultivation, propagation or the protection of genetic resources of rare, endemic and economically valuable plants [19][20][21][22]. In vitro culture of some Silene species such as micropropagation of S. cretacea [23], a Silene hybrid (S. polypetala x S. virginica) [24] and S. sangaria [25], suspension culture of S. vulgaris [26], shoot regeneration of S. vulgaris [27,28] and S. thymifolia [29] have been described previously. To date, there has been no report on the in vitro culture of S. bolanthoides.…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation of Silene bolanthoides Quézel, Contandr. & Pamukç. and assessment of genetic stability by flow cytometry

Çördük¹,

Yücel²,

Akıncı³

et al. 2018

ARCH BIOL SCI BELGRA

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Silene bolanthoides Quézel, Contandr. & Pamukç. is an endemic species from Kazdagi (Mt. Ida), Canakkale-Balikesir, Turkey. In order to develop an efficient shoot regeneration protocol, the leaf, nodal and internodal explants of S. bolanthoides were cultured on Murashige and Skoog (MS) medium containing benzyladenine (BA) alone or in combination with α-naphthaleneacetic acid (NAA). The highest number of regenerated shoots (5.75±0.1) was obtained from nodal explants that were cultured on MS medium with 8.8 µM BA+0.54 µM NAA. Regenerated shoots were rooted on MS medium without plant growth regulators (PGRs). Rooted plants (2-3 cm) were separately transferred to pots containing a mixture of peat and perlite (3:1 v/v) and acclimatized successfully in a growth chamber. Genetic stability of the propagated plants was assessed by flow cytometry and cytological analysis. Flow cytometry analysis demonstrated that regenerated plants had 2.61±0.01 pg nuclear DNA (2C) and seed-derived plants had on average 2.58±0.02 pg/2C. Cytological analysis showed that the regenerated plants had the same chromosome number as seed-derived plants of S. bolanthoides (2n=24). It was determined that regenerated plants were uniform in chromosome number and had a similar DNA content to the seed-derived ones, indicating that the described efficient shoot regeneration protocol can be applied for ex situ conservation of this species.

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Features of clonal micropropagation of Silene cretacea (Caryophyllaceae) in in vitro culture

Cited by 7 publications

References 10 publications

Structural, physiological and genetic diversification of Silene vulgaris ecotypes from heavy metal-contaminated areas and their synchronous in vitro cultivation

Structural, physiological and genetic diversification of Silene vulgaris ecotypes from heavy metal-contaminated areas and their synchronous in vitro cultivation

In vitro propagation and DNA barcode analysis of the endangered Silene schimperiana in Saint Katherine protectorate

In vitro propagation of Silene bolanthoides Quézel, Contandr. & Pamukç. and assessment of genetic stability by flow cytometry

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