Features of Human Microflora and Local Immunity in an Artificial Environment

Abstract

The article shows the role of extreme influences on the development of pathological changes in the dentomandibular system. The possibility of developing a system of preventive measures non-biocidal action, mainly using probiotics, in particular autoprobiotics is considered.Introduction. Artificially modified habitats are created by humans when they learn the nature of things that lie outside the everyday conditions of existence. This primarily concerns the exploration of space, the ocean and the Earth’s interior. To meet these challenges, artificial anthropo-ecosystems with modified habitat parameters are created. Under such altered conditions, the phylogenetically established coactant relationships of the human-micro-organism ecological system undergo changes. It takes the form of a syndrome of impaired colonization resistance [1].The aim of the work was to characterise the three main barriers to infection that form in humans: defence group microflora, covering tissues and immunity, in conditions of isolation in hermetically sealed objects with altered environments in long-duration diving, in conditions simulating several factors of space flight (isolation and hypokinesia) and in spaceflight conditions.Materials and methods. The determination of immunoglobulins in saliva was carried out using the nephelometric method. The content of immunoglobulins was studied in mixed unstimulated saliva obtained before meals. Monoclonal antibody solutions against sIgA, IgA, IgG antigens were used to determine immunoglobulin concentration. The traditional method of bacteriological analysis was used to identify microorganisms. Bacteriological analysis was performed by seeding on nutrient media (HiCrome Candida Agar, Staphylococcus Agar No10, Columbia Blood Agar Base, Columbia Blood Agar Base + Staph Strepto Supplement, Columbia Blood Agar +Non Spore Anaerobic Supplement).Results and discussion. Insulation in hyperbaric (up to 40 atmospheres) containment facilities.