FED-BATCH FERMENTATION OF<i>BACILLUS CLAUSII</i>FOR EFFICIENT PRODUCTION OF ALKALINE PROTEASE USING DIFFERENT FEEDING STRATEGIES

Tabandeh, Fatemeh; Moghaddam, H. R. Hosseinian; Yakhchali, Bagher; Shariati, Parvin; Mousavian, M T Hamed; Ghasemi, Fahimeh

doi:10.1080/00986445.2011.552024

Cited by 9 publications

(6 citation statements)

References 19 publications

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“…The optimization of the inoculum size has been reported to be quite important in that low density can result in a mitigation of enzyme secretion, owing to a drop in cell numbers, whereas the high inoculum density can attenuate enzyme production due to the competition for available nutrients . Similarly, the significant effects of cassava pulp and soybean meal have confirmed the results of prior studies that carbon and nitrogen sources have a significant influence on protease production . All other insignificant variables were set to certain values (as mentioned to central points), and the optimal levels of the three variables (cassava pulp, soybean meal, and inoculum size) were further explored with an RSM design.…”

Section: Resultsmentioning

confidence: 82%

Optimization of neutral protease production from Bacillus subtilis: Using agroindustrial residues as substrates and response surface methodology

Zhu

Cheng

Chen

et al. 2013

Biotech and App Biochem

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Statistically based experimental designs were applied to optimize the fermentation medium and cultural conditions for the maximization of neutral protease using three agroindustrial residues (cassava pulp, soybean meal, and wheat bran) and Bacillus subtilis DES-59. The Plackett-Burman design was used to evaluate the effects of variables such as the concentration of substrates, initial pH, shaker's rotating speed, temperature, inoculum size, and incubation time. Among the eight parameters, three significant variables (cassava pulp, soybean meal, and inoculum size) were selected for the optimization study, in which a central composite design was used to optimize the concentrations of cassava pulp and soybean meal and inoculum size and investigate the interactive effects of the three variables. The optimal parameters obtained from response surface methodology are 37.78 g/L of cassava pulp, 15 g/L of soybean meal, and 6.5% (v/v) of inoculum size, respectively, resulting in a maximum neutral protease activity of 4107 ± 122 U/mL.

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Section: Resultsmentioning

confidence: 82%

Optimization of neutral protease production from Bacillus subtilis: Using agroindustrial residues as substrates and response surface methodology

Zhu

Cheng

Chen

et al. 2013

Biotech and App Biochem

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show abstract

“…To enhance the cell density, it is necessary to extend the log phase in the fermentation process. Fed-batch processes have been shown to be the most effective at reducing undesirable effects like catabolize repression and by-product formation [25]. Nutrient feeding was used to overcome starvation and to extend the growth of P. pastoris.…”

Section: Fed-batch Fermentationmentioning

confidence: 99%

High cell density fermentation strategies for the production of bovine lactoferrin in Pichia pastoris

Trinh¹,

Ngo²,

Truong³

2023

J App Biol Biotech

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Bovine lactoferrin (bLf) has been well characterized as a multifunctional glycoprotein belonging to the transferrin family with antibacterial, antioxidant, anti-tumor, and antiviral activities. The main aim of this study was to determine the feeding strategies in fed-batch fermentation to obtain high-cell density in Pichia pastoris KM71H-3 for production of bLf. During the growth phase, two feeding strategies were performed: (i) Feeding glycerol solution; and (ii) feeding glycerol and nitrogen source solution with a carbon-to-nitrogen ratio of 3:1. In addition, two feeding induction strategies were applied: One-time feeding and continuous feeding with 0.5% of methanol every 24 h. The results showed that the highest cell density at OD 600 was 362.67 ± 2.04 when the feeding phase used a mix-feed solution of glycerol and nitrogen sources with a carbon to nitrogen ratio of 3:1 in the 2-L scale. Up-scaled production of lactoferrin from this strain was successfully employed in the 10-L and 100-L bioreactor with the highest OD 600 reaching 338.20 ± 3.38 and 375.50 ± 2.98, respectively. For optimal lactoferrin expression, methanol was fed continuously, corresponding to an induced methanol concentration of 0.5% per 24 h. The appropriate induction time was 48 h. This research provides information on cell growth and fed-batch strategies for enhanced bLf production using P. pastoris as a host, which may be applicable to the expression of other proteins from P. pastoris strains.

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“…Glucose had a negative effective coefficient while the other four variables showed positive effective coefficients (Figure 1). The use of glucose causes a decrease in pH of the fermentation medium [18,19], which could also inhibit alkaline strains such as B. clausii, especially when the experiments were not adjusted to pH 8.5 during fermentation process. Therefore, the addition of glucose could be avoided in further experiments.…”

Section: Glucosementioning

confidence: 99%

“…Bacillus clausii is an alkaline species [6,18], so the culture medium is usually brought to alkaline pH for its optimal growth. Tabandeh et al, launched the B. clausii at initial pH 10.5 without pH control [18].…”

Section: Batch Fermentationmentioning

confidence: 99%

Improvement of Bacillus clausii cell HH1 density via culture medium optimization and pH-stat fed batch fermentation

Pham,

Nguyen,

2023

Vietnam J. Sci. Technol.

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In this study, the culture medium and fermentation modes were studied aiming to improve the cell density of Bacillus clausii. Firstly, the factorial design method using Minimum Run Resolution IV design was used to evaluate the relative importance of culture medium components to the growth of Bacillus clausii. The results showed that three components peptone, yeast extract and malt extract were the components significantly affecting the bacterial biomass. Then, the optimization of these three ingredient concentrations using a response surface methodology with the Box-Behnken design resulted that the maximal biomass had been achieved using the medium containing 7.64 g/L peptone, 10 g/L yeast extract and 6.36 g/L malt extract. Finally, the pH-stat fed batch fermentation was conducted in a 2-liter bioreactor where the 9X concentrated optimal culture medium was feed into the bioreactor based on the pH signal. As a result, the microbial cell density increased by 2.9-fold compared to that achieved through batch fermentation.

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FED-BATCH FERMENTATION OFBACILLUS CLAUSIIFOR EFFICIENT PRODUCTION OF ALKALINE PROTEASE USING DIFFERENT FEEDING STRATEGIES

Cited by 9 publications

References 19 publications

Optimization of neutral protease production from Bacillus subtilis: Using agroindustrial residues as substrates and response surface methodology

Optimization of neutral protease production from Bacillus subtilis: Using agroindustrial residues as substrates and response surface methodology

High cell density fermentation strategies for the production of bovine lactoferrin in Pichia pastoris

Improvement of Bacillus clausii cell HH1 density via culture medium optimization and pH-stat fed batch fermentation

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