Feed, Microbiota, and Gut Immunity: Using the Zebrafish Model to Understand Fish Health

Nadal, Adrià López; Ikeda-Ohtsubo, Wakako; Sipkema, Detmer; Peggs, David; McGurk, Charles; Forlenza, Maria; Wiegertjes, G.F.; Brugman, Sylvia

doi:10.3389/fimmu.2020.00114

Cited by 175 publications

(104 citation statements)

References 98 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although a large body of studies has documented that the host innate immunity plays significant roles for shaping microbiota and vice versa ( 26 , 54 ), our current results imply that the compositional structure of microbiota is not strongly correlated to the expression patterns of host immune genes. Feeding of OM fucoidan for 3 weeks profoundly altered gut microbiota composition in adult zebrafish; however, the analysis of a selected set of immune genes only showed a slight reduction in the expression of il1b .…”

Section: Discussioncontrasting

confidence: 75%

“…Indeed, the unclassified Comamonadaceae [AB076847, (42)] and Shinella spp., the intestinal abundance of which increased in response to the fucoidan feeding of adult zebrafish in our current study, have been reported to belong to denitrifying bacteria possessing biodegrading abilities of diverse compounds including biopolymers and xenobiotics (42,52,53). Therefore, it seems plausible that OM fucoidan may serve as a carbon source for intestinal bacteria of adult zebrafish, and identification of the degradation pathways involved awaits further investigation Although a large body of studies has documented that the host innate immunity plays significant roles for shaping microbiota and vice versa (26,54), our current results imply that the compositional structure of microbiota is not strongly correlated to the expression patterns of host immune genes. Feeding of OM fucoidan for 3 weeks profoundly altered gut microbiota composition in adult zebrafish; however, the analysis of a selected set of immune genes only showed a slight reduction in the expression of il1b.…”

Section: Discussionmentioning

confidence: 59%

“…Considering possible interactions between microbiota and host immune responses, it is crucial to evaluate host immunity and microbiota simultaneously to elucidate the prebiotic potential OM fucoidan ( 18 ). Zebrafish offer an ideal in vivo model to investigate how fucoidan affects host immunity and microbiota under normal (unchallenged) conditions because of their compatibility with live visualization ( 26 ). Using a double-transgenic zebrafish model combined with next-generation sequencing of 16S rRNA genes, we have recently shown that microbiota modulation by antibiotics can significantly affect host inflammatory immune responses in zebrafish larvae immersed in saponin ( 27 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Intestinal Microbiota and Immune Modulation in Zebrafish by Fucoidan From Okinawa Mozuku (Cladosiphon okamuranus)

et al. 2020

Self Cite

View full text Add to dashboard Cite

Fucoidan represents fucose-rich sulfated polysaccharides derived from brown seaweeds, which exerts various biological activities applicable for functional foods and therapeutic agents. The objective of the present study was to investigate in vivo effects of fucoidan extracted from Okinawa mozuku (Cladosiphon okamuranus), common edible seaweed in Japan, on immune responses and microbiota composition in zebrafish. We treated larvae and adult zebrafish with Okinawa mozuku (OM) fucoidan by immersion (100 and 500 µg/mL, 3 days) and by feeding (3 weeks), respectively. The effect of OM fucoidan on immune responses in zebrafish larvae was evaluated by live imaging of neutrophils and macrophages as well as quantitative polymerase chain reaction of pro-and anti-inflammatory cytokine genes. Whole microbiota of zebrafish larvae and intestinal microbiota of adult zebrafish treated with OM fucoidan were analyzed by Illumina MiSeq pair-end sequencing of the V3-V4 region of 16S rRNA genes. Fucoidan treatment only slightly affected the composition of the larvae microbiota and the number of neutrophils and macrophages, while pro-and anti-inflammatory cytokine gene expression levels were upregulated in the larvae treated with 500 µg/mL OM fucoidan. In contrast, feeding of OM fucoidan clearly altered the intestinal microbiota composition of adult zebrafish, which was characterized by the emergence and predominance of multiple bacterial operational taxonomic units (OTUs) affiliated with Rhizobiaceae and Comamonadaceae at the expense of E. coli-related Enterobacteriaceae, the dominant OTUs throughout the studied samples. These changes were accompanied by decreased expression levels of pro-inflammatory cytokine il1b in the intestines of the adult zebrafish. Our current study provides the first insights into in vivo modulatory effects of fucoidan on microbiota and immune responses of unchallenged zebrafish, which underscores the potential of fucoidan to play a modulatory role in the diet-microbiota-host interplay.

show abstract

Section: Discussioncontrasting

confidence: 75%

Section: Discussionmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Intestinal Microbiota and Immune Modulation in Zebrafish by Fucoidan From Okinawa Mozuku (Cladosiphon okamuranus)

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…The immune system during the early life is immature, which has been considered as a major restriction to healthy growth of fish larvae ( 46 , 47 ). Probiotics can act as non-specific immune factors and have a positive effect on host immune system ( 35 , 48 ) CB supplementation significantly affected nearly all immune enzyme activities (ACP, AKP, CAT and LZM), indicating CB supplementation could improve immune status of larvae.…”

Section: Discussionmentioning

confidence: 99%

Early Life Intervention Using Probiotic Clostridium butyricum Improves Intestinal Development, Immune Response, and Gut Microbiota in Large Yellow Croaker (Larimichthys crocea) Larvae

Yin

Liu

et al. 2021

Front. Immunol.

View full text Add to dashboard Cite

Marine fish larvae are vulnerable during the early life period. The early intervention using probiotics may be a promising method to improve growth of fish larvae. In this study, a 30-day feeding trial was conducted to evaluate the effects of early life intervention using probiotic Clostridium butyricum (CB) on growth performance, intestinal development, immune response and gut microbiota of large yellow croaker (Larimichthys crocea) larvae. Four isonitrogenous and isolipidic diets were formulated with the supplementation of four different levels of CB (5 × 109 CFU g−1), 0.00% (Control), 0.10% (CB1), 0.20% (CB2), and 0.40% (CB3). Results showed that larvae fed diets with CB had significant higher final length than the control group. Meanwhile, larvae fed the diet with 0.10% CB had significant higher final weight and specific growth rate (SGR) than the control group. However, no significant difference in survival rate was observed among dietary treatments. CB supplementation significantly increased the height of intestinal villus and the length of intestinal enterocyte. Similarly, CB supplementation significantly increased the expression of tight zonula occludens-2 (zo-2) and ornithine decarboxylase (odc) than the control group. Larvae fed the diet with 0.20% CB had significant higher lipase and leucine-aminopeptidase (LAP) activity than the control group. Moreover, CB supplementation significantly improved immune enzyme activities than the control group. Sequencing of bacterial 16S rRNA V4-5 region indicated that dietary CB altered intestinal microbiota profile and decreased intestinal microbial diversities of larvae. CB supplementation could effectively increase the abundance of CB, and decrease the abundance of some potential pathogenic bacteria in larval gut. These results revealed that early life intervention using 0.10–0.20% CB could promote growth of large yellow croaker larvae probably through promoting intestinal development, improving immune enzyme activities and modulating gut microbiota.

show abstract

“…However, the precise mechanisms by which diet in particular can influence host-microbe immune interactions and their impact on fish health remain largely unexplored [6,7]. Zebrafish serve as an excellent model for examining the complex interaction of diet microbiome and the immune function due to a shorter life cycle, high presence of human orthologous genes, and availability of a large array of established genomic tools [8,9].…”

Section: Introductionmentioning

confidence: 99%

Dietary Supplementation with Omega-6 LC-PUFA-Rich Microalgae Regulates Mucosal Immune Response and Promotes Microbial Diversity in the Zebrafish Gut

Nayak

Al-Ashhab

Zilberg

et al. 2020

Biology

View full text Add to dashboard Cite

The effect of dietary omega-6 long-chain polyunsaturated fatty acid (LC-PUFA) on host microbiome and gut associated immune function in fish is unexplored. The effect of dietary supplementation with the omega-6 LC-PUFA-rich microalga Lobosphaera incisa wild type (WT) and its delta-5 desaturase mutant (MUT), rich in arachidonic-acid and dihomo-gamma-linolenic acid (DGLA), respectively, on intestinal gene expression and microbial diversity was analyzed in zebrafish. For 1 month, fish were fed diets supplemented with broken biomass at 7.5% and 15% (w/w) of the two L. incisa strains and a control nonsupplemented commercial diet. Dietary supplementation resulted in elevated expression of genes related to arachidonic acid metabolism-cyclooxygenase 2 (cox-2), lipoxygenase 1(lox-1), anti-inflammatory cytokine-interleukin 10 (il-10), immune defense-lysozyme (lys), intestinal alkaline phosphatase (iap), complement (c3b), and antioxidants-catalase (cat), glutathione peroxidase (gpx). Microbiome analysis of the gut showed higher diversity indices for microbial communities in fish that were fed the supplemented diets compared to controls. Different treatment groups shared 237 operational taxonomic units (OTUs) that corresponded to the core microbiome, and unique OTUs were evident in different dietary groups. Overall, the zebrafish gut microbiome was dominated by the phylum Fusobacteria and Proteobacteria (averaging 38.4% and 34.6%, respectively), followed by Bacteroidetes (12.9%), Tenericutes, Planctomycetes, and Actinobacteria (at 3.1–1.3%). Significant interaction between some of the immune-related genes and microbial community was demonstrated.

show abstract

Feed, Microbiota, and Gut Immunity: Using the Zebrafish Model to Understand Fish Health

Cited by 175 publications

References 98 publications

Intestinal Microbiota and Immune Modulation in Zebrafish by Fucoidan From Okinawa Mozuku (Cladosiphon okamuranus)

Intestinal Microbiota and Immune Modulation in Zebrafish by Fucoidan From Okinawa Mozuku (Cladosiphon okamuranus)

Early Life Intervention Using Probiotic Clostridium butyricum Improves Intestinal Development, Immune Response, and Gut Microbiota in Large Yellow Croaker (Larimichthys crocea) Larvae

Dietary Supplementation with Omega-6 LC-PUFA-Rich Microalgae Regulates Mucosal Immune Response and Promotes Microbial Diversity in the Zebrafish Gut

Contact Info

Product

Resources

About