Feed Supplements Production, Microbiological Production of Beta-Carotene in Shaken Flasks

Anderson, Ronald D.; Arnold, Margie; Nelson, George E.; Ciegler, A.

doi:10.1021/jf60089a009

Cited by 61 publications

(19 citation statements)

References 3 publications

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“…Blakeslea was chosen for the development of industrial carotene production because its mated cultures contain much more ␤-carotene than single cultures, whether grown on agar or in liquid medium (1). Sexual stimulation can be mimicked by the addition of trisporic acids to single cultures (5), but industry finds the costs too high.…”

Section: Discussionmentioning

confidence: 99%

Mutants and Intersexual Heterokaryons of Blakeslea trispora for Production of β-Carotene and Lycopene

Mehta

Obraztsova

Cerdá‐Olmedo

2003

Appl Environ Microbiol

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The industrial production of ␤-carotene with the zygomycete Blakeslea trispora involves the joint cultivation of mycelia of opposite sex in the presence of ␤-ionone and other chemical activators. We have obtained improved strains by mutation and heterokaryosis. We chose wild strains on the basis of their growth and carotene content in single and mated cultures. Following exposure of their spores to N-methyl-N-nitro-Nnitrosoguanidine, we obtained high-carotene mutants, which were more productive than their parents but similar to them in having ␤-carotene as the main product. Further increases in carotene content were obtained after a new round of mutagenesis in one of the mutants. The production was shifted to lycopene in cultures incubated in the presence of nicotine and in lycopene-rich mutants derived from the wild strains. The highest production levels were achieved in intersexual heterokaryons, which contained mutant nuclei of opposite sex. These contained up to 39 mg of ␤-carotene or 15 mg of lycopene per g (dry mass) under standard laboratory conditions in which the original wild strains contained about 0.3 mg of ␤-carotene per g (dry mass). ␤-Ionone did not increase the carotene content of these strains. Not all wild strains lent themselves to these improvements, either because they produced few mutants or because they did not increase their carotene production in mated cultures.

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Section: Discussionmentioning

confidence: 99%

Mutants and Intersexual Heterokaryons of Blakeslea trispora for Production of β-Carotene and Lycopene

Mehta

Obraztsova

Cerdá‐Olmedo

2003

Appl Environ Microbiol

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“…4), the intermediates being phytofluene (7,8,11,12,7',8'-hexahydro^,i|i-carotene), ζ-carotene (7,8,7',8'-tetrahydro>^-carotene), and neurosporene (7,8-dihydro--ψ,ψ-carotene). In some fungi, the desaturation proceeds one stage further to yield 3,4dehydrolycopene (3,204). In some fungi, the desaturation proceeds one stage further to yield 3,4dehydrolycopene (3,204).…”

Section: B Desaturation Of Phytoenementioning

confidence: 99%

Regulation of Carotenoid Biosynthesis

Bramley

Mackenzie

1988

Current Topics in Cellular Regulation

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“…In our experiments the amount of trisporic acid was high because the percentage of zygospores in the total biomass dry weight was high (11.44%, Table 3) and this compound covered the space in which the mevalonate kinase, an enzyme responsible for the biosynthesis of the pigment, is activated. Anderson et al (1958) reported that the addition of b-ionone to the medium increased the production of the pigment under conditions of low b-carotene production (19.8 mg L À1 ) indicating that the addition of b-ionone improves the . Effect of feeding medium composition on kinetic parameters of synthetic medium fermentation by B. trispora in fed-batch culture.…”

Section: Effect Of Feeding Medium Compositionmentioning

confidence: 99%

Production of Beta-Carotene from Synthetic Medium byBlakeslea trisporain Fed-batch Culture

2004

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The production of b-carotene from a synthetic medium by Blakeslea trispora in fed-batch culture was investigated. A maximum b-carotene concentration of 85.0 mg L À1 with productivity of 0.16 mg L À1 h À1 and specific b-carotene production rate of 0.01 mg g À1 h À1 was obtained by feeding the cells constantly with olive oil, cottonseed oil, soybean oil, antioxidant, and low concentration of growth factors at feeding rate of 4.2 mL h À1 from the beginning of the fermentation. In this case, the fed-batch culture supported high values of biomass dry weight (11.0 g L À1 ) and sugar utilization (0.976 g g À1 ). The morphology of the fungus was studied during growth in fed-batch fermentation system using an image analysis system. Zygospores are the morphological forms, which are responsible for the production of the pigment. The highest percentage of zygospores (11.44%) was correlated with the highest percentage of intracellular b-carotene (0.72%) in the total biomass dry weight. Moreover, high percentages of vacuolated hyphae, evacuated cells, and degenerated hyphae of the microorganism were observed. This was due to the formation of high amount of H 2 O 2 by exposure of the microorganism to high dissolved oxygen concentration.

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Feed Supplements Production, Microbiological Production of Beta-Carotene in Shaken Flasks

Cited by 61 publications

References 3 publications

Mutants and Intersexual Heterokaryons of Blakeslea trispora for Production of β-Carotene and Lycopene

Mutants and Intersexual Heterokaryons of Blakeslea trispora for Production of β-Carotene and Lycopene

Regulation of Carotenoid Biosynthesis

Production of Beta-Carotene from Synthetic Medium byBlakeslea trisporain Fed-batch Culture

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