Feedback Control of Redox Potential in Hybridoma Cell Culture

Meneses, Ana Maria; Gómez, A.; Ramı́rez, Octavio T.

doi:10.1007/0-306-46875-1_4

Cited by 2 publications

(2 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since the redox potential is influenced by pH and DO [30], it is affected by the same factors of the DoE (Table 4). The redox potential of the media before inoculation has been shown before to correlate with growth rates in animal cell culture [36] and lower culture redox potential led to higher specific growth rates and mAb concentrations in a hybridoma process [37]. Whilst reducing conditions can have a positive effect on cell growth and product titer, it might further decrease mAb galactosylation [38].…”

Section: Impact On Physico-chemical Media Characteristics Including Lmentioning

confidence: 98%

Towards robust cell culture processes — Unraveling the impact of media preparation by spectroscopic online monitoring

Brunner

Brosig

Losing

et al. 2019

Engineering in Life Sciences

View full text Add to dashboard Cite

Biopharmaceutical manufacturing processes can be affected by variability in cell culture media, e.g. caused by raw material impurities. Although efforts have been made in industry and academia to characterize cell culture media and raw materials with advanced analytics, the process of industrial cell culture media preparation itself has not been reported so far. Within this publication, we first compare mid‐infrared and two‐dimensional fluorescence spectroscopy with respect to their suitability as online monitoring tools during cell culture media preparation, followed by a thorough assessment of the impact of preparation parameters on media quality. Through the application of spectroscopic methods, we can show that media variability and its corresponding root cause can be detected online during the preparation process. This methodology is a powerful tool to avoid batch failure and is a valuable technology for media troubleshooting activities. Moreover, in a design of experiments approach, including additional liquid chromatography–mass spectrometry analytics, it is shown that variable preparation parameters such as temperature, power input and preparation time can have a strong impact on the physico‐chemical composition of the media. The effect on cell culture process performance and product quality in subsequent fed‐batch processes was also investigated. The presented results reveal the need for online spectroscopic methods during the preparation process and show that media variability can already be introduced by variation in media preparation parameters, with a potential impact on scale‐up to a commercial manufacturing process.

show abstract

Section: Impact On Physico-chemical Media Characteristics Including Lmentioning

confidence: 98%

Towards robust cell culture processes — Unraveling the impact of media preparation by spectroscopic online monitoring

Brunner

Brosig

Losing

et al. 2019

Engineering in Life Sciences

View full text Add to dashboard Cite

show abstract

“…?75 mV for many cell lines) values (Griffiths 2000), although Eh values can vary considerably even when tried to be kept constant (Meneses et al 2000). Redox potential is not only affected by oxygen, pH and medium composition, but to a significant extent also by the rate of generation of reductants by cells (Pluschkell and Flickinger 1995-96).…”

Section: Redox Conversionmentioning

confidence: 98%

Arsenic trioxide versus tetraarsenic oxide in biomedical research: misunderstandings and misinterpretations

2011

View full text Add to dashboard Cite

This work presents an analytical chemist's view on the sometimes unconscious use of arsenic trioxide in (bio)medical research. Arsenic trioxide is a frequently used chemical in cancer treatment research and its action to various malignant cells has been extensively studied and published. Unfortunately some research articles show trivial errors with regards to background knowledge of the chemical, handling the chemical, experimental design and interpretation of results like e.g. in a range of articles comparing advantages of tetraarsenic oxide over arsenic trioxide (dimeric/monomeric) although the dissolution of both yields the same active compound (HAsO(2)). To fully understand the implications of these errors we will highlight some of them with the intent to harmonize future work in this field.

show abstract

Feedback Control of Redox Potential in Hybridoma Cell Culture

Cited by 2 publications

References 14 publications

Towards robust cell culture processes — Unraveling the impact of media preparation by spectroscopic online monitoring

Towards robust cell culture processes — Unraveling the impact of media preparation by spectroscopic online monitoring

Arsenic trioxide versus tetraarsenic oxide in biomedical research: misunderstandings and misinterpretations

Contact Info

Product

Resources

About