Feeding recombinant E. coli with GST-mBmKTX fusion protein increases the fecundity and lifespan of Caenorhabditis elegans

Xu, Jie; Jiang, Yajie; Wan, Lu; Wang, Qi; Huang, Zebo; Liu, Yongmei; Wu, Yingliang; Chen, Zongyun; Liu, Xin

doi:10.1016/j.peptides.2017.01.003

Cited by 8 publications

(9 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, the results showed that no statistically significant differences were found between the control and orange extract treatment on fecundity, which meant orange extracts did not change the reproductive capacity while extending the lifespan of C. elegans. A series of physiological functions, including the locomotor capacity, age pigment, and body length, have a connection with the lifespan [28,29]. We observed that worms supplemented with orange extracts showed more youthful and energetic mobility than control worms at different stages of the life process.…”

Section: Discussionmentioning

confidence: 75%

See 1 more Smart Citation

Effects of Orange Extracts on Longevity, Healthspan, and Stress Resistance in Caenorhabditis elegans

et al. 2020

View full text Add to dashboard Cite

Orange, with various bioactive phytochemicals, exerts various beneficial health effects, including anti-cancer, antioxidant, and anti-inflammatory properties. However, its anti-aging effects remain unclear. In this study, the Caenorhabditis elegans (C. elegans) model was used to evaluate the effects of orange extracts on lifespan and stress resistance. The results indicated that orange extracts dose-dependently increased the mean lifespan of C. elegans by 10.5%, 18.0%, and 26.2% at the concentrations of 100, 200, and 400 mg/mL, respectively. Meanwhile, orange extracts promoted the healthspan by improving motility, and decreasing the accumulation of age pigment and intracellular reactive oxygen species (ROS) levels without damaging fertility. The survival rates of orange extract-fed worms were obviously higher than those of untreated worms against thermal and ultraviolet-B (UV-B) stress. Moreover, the activities of superoxide dismutase (SOD) and catalase (CAT) were significantly enhanced while malondialdehyde (MDA) contents were diminished. Further investigation revealed that worms supplemented with orange extracts resulted in upregulated levels of genes, including daf-16, sod-3, gst-4, sek-1, and skn-1, and the downregulation of age-1 expression. These findings revealed that orange extracts have potential anti-aging effects through extending the lifespan, enhancing stress resistance, and promoting the healthspan.

show abstract

Section: Discussionmentioning

confidence: 75%

“…Gravid nematodes at the L4 stage were allotted to NGM plates without FUDR to examine the reproduction capacity [28]. Only one nematode was located on each plate.…”

Section: Reproduction Assaymentioning

confidence: 99%

Effects of Orange Extracts on Longevity, Healthspan, and Stress Resistance in Caenorhabditis elegans

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Previous studies have linked the entirety of the gene to a receptor based on binding studies and full transgenic rescue 28, 66,69 . Here, we employ a rescue-by-feeding assay, following the design of RNAi feeding protocols, to rescue individual peptides 36 . While "feeding" of peptides through soaking is a valid approach, there are many constraints on such approaches, the most prominent being the ability to acquire purified peptides 28,66 .…”

Section: Discussionmentioning

confidence: 99%

“…To identify which FLP-3 peptides are required for male avoidance of ascr#8, we adopted a peptide feeding approach, similar to RNAi feeding, as initially described previously 36 . Using Gateway Cloning technology, we first inserted the peptide coding sequences for the FLP-3 peptides unable to rescue the ascr#8 response in the ok3625 allele (Supp.…”

Section: Rescue Of Individual Flp-3 Peptides By Feeding Reveals a Spementioning

confidence: 99%

Distinct Neuropeptide-Receptor Modules Regulate a Sex-Specific Behavioral Response to a Pheromone

Reilly

McGlame

Vandewyer³

et al. 2020

Preprint

View full text Add to dashboard Cite

Dioecious species are a hallmark of the animal kingdom, with opposing sexes responding differently to identical sensory cues. Here, we study the response of C. elegans’ to the small-molecule pheromone, ascr#8, which elicits opposing behavioral valences in each sex. We identify a novel neuropeptide-neuropeptide receptor (NP/NPR) module that is active in males, but not in hermaphrodites. Using a novel paradigm of neuropeptide rescue that we established, we leverage bacterial expression of individual peptides to rescue the sex-specific response to ascr#8. Concurrent biochemical studies confirmed individual FLP-3 peptides differentially activate two divergent receptors, NPR-10 and FRPR-16. Interestingly, the two of the peptides that rescued behavior in our feeding paradigm are related through a conserved threonine, suggesting that a specific NP/NPR combination sets a male state, driving the correct behavioral valence of the ascr#8 response. Receptor expression within pre-motor neurons reveals novel coordination of male-specific and core locomotory circuitries.

show abstract

“…Our novel technology to rescue peptide function is advantageous over transgenic studies, as it allows for functional characterization of individual peptides. This genetic tool is built off the principles of RNAi feeding techniques to supply worms with the peptide of interest through their food source as previously shown for the scorpion venom peptide mBmKTX to alter lifespan and egg-laying behavior in C. elegans 38 . More recently, we have improved upon this paradigm to characterize the FMRFamide-like peptide gene, flp-3 61 .…”

Section: Differential Functional Activity Of Peptides Encoded By Pigment Dispersing Factor (Pdf)-1mentioning

confidence: 99%

Non-Transgenic Functional Rescue of Neuropeptides

DiLoreto

Reilly

Srinivasan

2021

Preprint

View full text Add to dashboard Cite

Animals constantly respond to changes in their environment and internal states via neuromodulation. Neuropeptide genes modulate neural circuits by encoding either multiple copies of the same neuropeptide or different neuropeptides. This architectural complexity makes it difficult to determine the function of discrete and active neuropeptides. Here, we present a novel genetic tool that facilitates functional analysis of individual peptides. We engineered Escherichia coli bacteria to express active peptides and fed loss-of-function Caenorhabditis elegans to rescue gene activity. Using this approach, we rescued the activity of different neuropeptide genes with varying lengths and functions: trh-1, ins-6, and pdf-1. While some peptides are functionally redundant, others exhibited unique and previously uncharacterized functions. The mechanism of peptide uptake is reminiscent of RNA interference, suggesting convergent mechanisms of gene regulation in organisms. Our rescue-by-feeding paradigm provides a high-throughput screening strategy to elucidate the functional landscape of neuropeptide genes regulating different behavioral and physiological processes.

show abstract

Feeding recombinant E. coli with GST-mBmKTX fusion protein increases the fecundity and lifespan of Caenorhabditis elegans

Cited by 8 publications

References 43 publications

Effects of Orange Extracts on Longevity, Healthspan, and Stress Resistance in Caenorhabditis elegans

Effects of Orange Extracts on Longevity, Healthspan, and Stress Resistance in Caenorhabditis elegans

Distinct Neuropeptide-Receptor Modules Regulate a Sex-Specific Behavioral Response to a Pheromone

Non-Transgenic Functional Rescue of Neuropeptides

Contact Info

Product

Resources

About