Feeding regimes modulate biomarkers responsiveness in mussels treated with diclofenac

The Potential Risk of Electronic Waste Disposal into Aquatic Media: The Case of Personal Computer Motherboards

“…Algae, representing key producers in the aquatic ecosystems, are ideal biological models for alarming disturbances in water quality due to the presence of different types of chemicals, wastes and leachates ([ 35 , 36 , 37 , 51 , 67 ]. To this end, algae cultured in PCMB leachate-utilized media showed differential responses in their growth and algal cell densities, thus revealing the deterioration of culture media due to chemical substances leaching from PCMB.…”

“…(strain SAG 22.83), and Scenedesmus rubescens (strain SAG 5.95) (Göttingen, Germany), as well as the saltwater species Dunaliella tertiolecta (CCAP19/6B) and Tisochrysis lutea (T. ISO strain CCAP 927, formerly listed as Isochrysis sp., Isochrysis galbana ) were used for performing algal bioassays. In all cases, fresh- and salt-water algal strains were maintained and cultured in sterilized flasks, containing filtered DW enriched with BG-11 medium (24 ± 1 °C, pH 8.3 ± 0.3), and filtered ASW enriched with f/2 medium without Si (20 ± 1 °C, pH 8.3 ± 0.3, salinity 35) [ 35 , 36 ], under constant illumination (4300 lx). At the late logarithmic phase, a proper amount of each algal strain (1 × 10 4 cells mL −1 ) was transferred in conical sterilized flasks (volume 200 mL), containing PCBM-free (control cultures) or PCMB-utilized culture media (BG-11 and f/2 prepared with appropriate portions of filtered PCBM leachate in each case).…”

“…Mussel species Mytilus galloprovincialis (5–6 cm long, almost 1 year old) were collected from a mussel farm, located in a well-monitored and characterized nature reserve area (north side of Korinthiakos Gulf, Galaxidi, Greece). The selection of the current area was based on previous studies that showed negligible levels of inorganic and organic pollution [ 36 , 38 , 39 ]. Mussel collection was performed in February 2020, thus avoiding any interference of spawning with the obtained stress indices [ 36 , 40 ].…”

“…The selection of the current area was based on previous studies that showed negligible levels of inorganic and organic pollution [ 36 , 38 , 39 ]. Mussel collection was performed in February 2020, thus avoiding any interference of spawning with the obtained stress indices [ 36 , 40 ].…”