As the world grapples with the challenges of climate change and the depletion of fossil fuel reserves, the transition towards sustainable transportation has become imperative. Various alternative energy options to enhance sustainable transportation such as solar, wind, hydroelectric, biofuels, and hydrogen, highlighting their potential to mitigate greenhouse gas emissions needs to be explored. Real-world cases drawn from various international contexts were examined to offer important insights into how sustainable mobility practices may be supported by alternative energy solutions. Some challenges in transitioning to sustainable transport systems which include energy efficiency, scalability, infrastructure needs, and environmental impact were identified. This introduction explores the potential of alternative energy sources in enhancing sustainable transportation, focusing on their advantages, challenges, and transformative impact on promoting greener, more equitable, and efficient mobility solutions. The remainder of the chapter is described as follows: Section 2 presents the electric vehicles (EVs) applications. Section 3 reviews hydrogen fuel technology. Section 4 examines the biofuels energy. Section 5 presents the environmental impacts and benefits of alternative energy sources. Section 6 explains ways of overcoming challenges and adoption while Section 7 finally concludes the chapter by discussing case studies and best practices on alternative energy sources.