Femto-molar sensitive field effect transistor biosensors based on silicon nanowires and antibodies

Puppo, Francesca; Doucey, Marie‐Agnès; Moh, T. S. Y.; Pandraud, G.; Sarro, P.M.; Micheli, Giovanni De; Carrara, Sandro

doi:10.1109/icsens.2013.6688205

Cited by 30 publications

(19 citation statements)

References 19 publications

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“…6), thus confirming the detection of occurring immuno-recognition events between antibody and antigen molecules. An according shift of the threshold voltage (V th ) was also observed in the I ds -V bg curves [14]. The results confirm data in literature about the possibility of sensing pathogenic factors with SiNW-FET sensors.…”

Section: Sensing Of Cancer Biomarkers In Ideal Conditionssupporting

confidence: 78%

SiNW-FET in-Air Biosensors for High Sensitive and Specific Detection in Breast Tumor Extract

et al. 2016

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Abstract-The sensitive analysis of proteins is central to disease diagnosis. The detection and investigation of angiogenic and inflammatory ligands in the tumor tissue can further improve the level of knowledge of the cancer disease by capturing the heterogeneity and the complexity of the tumor microenvironment. In previous works we demonstrated that high quality Silicon Nanowire Field Effect Transistors (SiNW-FETs) can be used to sense very low concentration (fM) of pathogenic factors in controlled Phosphate Buffered Saline (PBS). In this work, we show SiNW-FETs as biosensors for the detection of cancer markers in tumor extracts, as proof of our technology to successfully work on real patients' sample. In particular, we achieved the detection of exogenously added rabbit antigen in a much more complex environment, i.e. a human breast tumor extract. Our results show specific and high sensitive antigen detection with p-type SiNWFETs in the femto-molar range. Further and most importantly, the wires sense rabbit antigen molecules in the presence of a 100.000 mass excess of non-specific protein, indicating that the sensor is extremely resistant to noise.

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Section: Sensing Of Cancer Biomarkers In Ideal Conditionssupporting

confidence: 78%

SiNW-FET in-Air Biosensors for High Sensitive and Specific Detection in Breast Tumor Extract

et al. 2016

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“…e l s e v i e r . c o m / l o c a t e / s u s c measurements [29]. In particular, we believe that the detection of hydrogen ions of a salt solution deposited and dried onto the device occurs thanks to the formation of a liquid thin film on the sample surface by adsorption of water molecules from the surrounding environment to the native oxide of the nanowire.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

“…Source to drain I ds -V ds characteristics were registered at a fixed back-gate potential V bg of 0 V. I ds -V ds curves were acquired in the linear scale by sweeping the source-to-drain potential from 0 to 5 V. This data was used to study the varying conductance [31], calculated as the slope of the curve, with varying pH. Current to voltage characteristics were also acquired in the logarithmic scale while double sweeping V ds was between −5 and +5 V. These second measurements enabled us to observe the changing hysteresis properties of the memristive sensors as a function of the ion concentration.…”

Section: Electrical Measurementsmentioning

confidence: 99%

Memristive sensors for pH measure in dry conditions

et al. 2014

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Keywords:Memristive sensor Silicon nanowires pH sensing Hysteresis Measurements in air Conductivity in thin water film A large progress in pH sensing with nanowire based ion-sensitive field-effect transistors (ISFETs) has been demonstrated over the years. The electrochemical reactions occurring at the wire surface-to-electrolyte interface play a key role in the detection of ions. In this letter, we show that pH sensing can also be performed on dried samples, through electrical measurements in air with a new kind of memristive sensor. The detection of different concentrations of [H + ] is confirmed by both the increased conductance and hysteretic voltage gap of the wires. The observed change in the electrical properties with pH in dry conditions is related to the formation of a wet film at the nanowire surface. Ions from the initial solution are free to move in the final water thin film at the sensing interface with consequent polarization of the NW surface.

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“…Puppo et al got rid of the Debye screening problem by performing the electrical measurements on dried samples after the antigen-binding coupling [98]. The authors developed a SiNW-FET sensor for the detection of vascular endothelial growth factor (VEGF), a pathological angiogenesis factor.…”

Section: Trends In Fet-based Immunosensors Using Nanomaterials As Senmentioning

confidence: 99%

Recent Trends in Field-Effect Transistors-Based Immunosensors

Moraes

Kubota

2016

Chemosensors

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Abstract:Immunosensors are analytical platforms that detect specific antigen-antibody interactions and play an important role in a wide range of applications in biomedical clinical diagnosis, food safety, and monitoring contaminants in the environment. Field-effect transistors (FET) immunosensors have been developed as promising alternatives to conventional immunoassays, which require complicated processes and long-time data acquisition. The electrical signal of FET-based immunosensors is generated as a result of the antigen-antibody conjugation. FET biosensors present real-time and rapid response, require small sample volume, and exhibit higher sensitivity and selectivity. This review brings an overview on the recent literature of FET-based immunosensors, highlighting a diversity of nanomaterials modified with specific receptors as immunosensing platforms for the ultrasensitive detection of various biomolecules.

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Femto-molar sensitive field effect transistor biosensors based on silicon nanowires and antibodies

Cited by 30 publications

References 19 publications

SiNW-FET in-Air Biosensors for High Sensitive and Specific Detection in Breast Tumor Extract

SiNW-FET in-Air Biosensors for High Sensitive and Specific Detection in Breast Tumor Extract

Memristive sensors for pH measure in dry conditions

Recent Trends in Field-Effect Transistors-Based Immunosensors

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