Femtogram Level Sensitivity achieved by Surface Engineered Silica Nanoparticles in the Early Detection of HIV Infection

Chunduri, L. A. Avinash; Kurdekar, Aditya; Haleyurgirisetty, Mohan; Bulagonda, Eswarappa Pradeep; Venkataramaniah, K.; Hewlett, Indira

doi:10.1038/s41598-017-07299-1

Cited by 31 publications

(20 citation statements)

References 42 publications

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“…We diluted the healthy human serum 100 times, which in turn reduced the sample amount required for analysis [50]. We added three different amounts of NP antigen to the diluted serum to give the different final concentrations; the recovery values are shown in Table 2.…”

Section: Detection Of Np In Human Serummentioning

confidence: 99%

Enzyme-Antibody-Modified Gold Nanoparticle Probes for the Ultrasensitive Detection of Nucleocapsid Protein in SFTSV

Yuqin

Zhao

et al. 2020

IJERPH

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As humans and climate change continue to alter the landscape, novel disease risk scenarios have emerged. Sever fever with thrombocytopenia syndrome (SFTS), an emerging tick-borne infectious disease first discovered in rural areas of central China in 2009, is caused by a novel bunyavirus (SFTSV). The potential for SFTS to spread to other countries in combination with its high fatality rate, possible human-to-human transmission, and extensive prevalence among residents and domesticated animals in endemic regions make the disease a severe threat to public health. Because of the lack of preventive vaccines or useful antiviral drugs, diagnosis of SFTS is the key to prevention and control of the SFTSV infection. The development of serological detection methods will greatly improve our understanding of SFTSV ecology and host tropism. We describe a highly sensitive protein detection method based on gold nanoparticles (AuNPs) and enzyme-linked immunosorbent assay (ELISA)—AuNP-based ELISA. The optical sensitivity enhancement of this method is due to the high loading efficiency of AuNPs to McAb. This enhances the concentration of the HRP enzyme in each immune sandwich structure. The detection limit of this method to the nucleocapsid protein (NP) of SFTSV was 0.9 pg mL−1 with good specificity and reproducibility. The sensitivity of AuNP-based ELISA was higher than that of traditional ELISA and was comparable to real-time quantitative polymerase chain reaction (qRT-PCR). The probes are stable for 120 days at 4 °C. This can be applied to diagnosis and hopefully can be developed into a commercial ELISA kit. The ultrasensitive detection of SFTSV will increase our understanding of the distribution and spread of SFTSV, thus helping to monitor the changes in tick-borne pathogen SFTSV risk in the environment.

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Section: Detection Of Np In Human Serummentioning

confidence: 99%

Enzyme-Antibody-Modified Gold Nanoparticle Probes for the Ultrasensitive Detection of Nucleocapsid Protein in SFTSV

Yuqin

Zhao

et al. 2020

IJERPH

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“…Thus, the concentration of p24 present in the sample determines the signal intensity. The fluorescence signal intensity was then recorded using a spectrophotometer (SpectraMax M5 microplate reader) ( 24 ). The calibration plot for the signal intensity versus the concentration of purified HIV-1 p24 antigen was plotted with the measured data, as depicted in Fig.…”

Section: Resultsmentioning

confidence: 99%

Streptavidin-conjugated gold nanoclusters as ultrasensitive fluorescent sensors for early diagnosis of HIV infection

Kurdekar

Chunduri²,

Manohar

et al. 2018

Sci. Adv.

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“…The ratiometric signal between the carbon dots and FITC was used for signal transduction across a linear range of 0.317-35 μg/dL (Wu et al 2016). The doping of europium into silica NPs has also been reported (Tagaya et al 2011) and has been shown to be capable of facilitating HIV p24 antigen detection in clinical samples (Chunduri et al 2017).…”

Section: Immunoassays and Biosensorsmentioning

confidence: 99%

Dye-doped silica nanoparticles: synthesis, surface chemistry and bioapplications

et al. 2020

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Background: Fluorescent silica nanoparticles have been extensively utilised in a broad range of biological applications and are facilitated by their predictable, wellunderstood, flexible chemistry and apparent biocompatibility. The ability to couple various siloxane precursors with fluorescent dyes and to be subsequently incorporated into silica nanoparticles has made it possible to engineer these fluorophores-doped nanomaterials to specific optical requirements in biological experimentation. Consequently, this class of nanomaterial has been used in applications across immunodiagnostics, drug delivery and human-trial bioimaging in cancer research. Main body: This review summarises the state-of-the-art of the use of dye-doped silica nanoparticles in bioapplications and firstly accounts for the common nanoparticle synthesis methods, surface modification approaches and different bioconjugation strategies employed to generate biomolecule-coated nanoparticles. The use of dye-doped silica nanoparticles in immunoassays/biosensing, bioimaging and drug delivery is then provided and possible future directions in the field are highlighted. Other non-cancerrelated applications involving silica nanoparticles are also briefly discussed. Importantly, the impact of how the protein corona has changed our understanding of NP interactions with biological systems is described, as well as demonstrations of its capacity to be favourably manipulated. Conclusions: Dye-doped silica nanoparticles have found success in the immunodiagnostics domain and have also shown promise as bioimaging agents in human clinical trials. Their use in cancer delivery has been restricted to murine models, as has been the case for the vast majority of nanomaterials intended for cancer therapy. This is hampered by the need for more human-like disease models and the lack of standardisation towards assessing nanoparticle toxicity. However, developments in the manipulation of the protein corona have improved the understanding of fundamental bio-nano interactions, and will undoubtedly assist in the translation of silica nanoparticles for disease treatment to the clinic.

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Femtogram Level Sensitivity achieved by Surface Engineered Silica Nanoparticles in the Early Detection of HIV Infection

Cited by 31 publications

References 42 publications

Enzyme-Antibody-Modified Gold Nanoparticle Probes for the Ultrasensitive Detection of Nucleocapsid Protein in SFTSV

Enzyme-Antibody-Modified Gold Nanoparticle Probes for the Ultrasensitive Detection of Nucleocapsid Protein in SFTSV

Streptavidin-conjugated gold nanoclusters as ultrasensitive fluorescent sensors for early diagnosis of HIV infection

Dye-doped silica nanoparticles: synthesis, surface chemistry and bioapplications

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