Fenoxaprop‐ethyl affects mouse oocyte quality and the underlying mechanisms

He, Yating; Yang, Leilei; Zhao, Yong; Shen, Wei; Yin, Shen; Sun, Qing‐Yuan

doi:10.1002/ps.5190

Cited by 15 publications

(6 citation statements)

References 36 publications

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“…The results revealed that the serum TG and TC levels were significantly increased in the tumor-bearing group compared with in the control group, alongside the number of abnormal oocytes. The abnormal rate of oocytes of the tumor-bearing group (57.10%) was increased compared with that of the control group (41.90%), although the number of abnormal oocytes in the control group was higher than that in a previous report (39 abnormal oocytes out of 93 oocytes) (16), which may be associated with the batch deference of mice. It was previously reported that the growth and survival of tumor cells depended on lipid metabolism, as the lipids could provide the energy for the proliferation of tumor cells (17).…”

Section: Discussioncontrasting

confidence: 68%

“…3). The number of normal and abnormal oocytes was counted; the results revealed that there were 39 abnormal oocytes out of a total of 93 oocytes in the control group, which was higher than that of a previous study (16), while there were 36 abnormal oocytes out of a total of 63 oocytes in the tumor-bearing group (Table II). The abnormal rate of oocytes in the tumor-bearing group (57.10%) was increased compared with that in the control group (41.90%) (Table II).…”

Section: Effects Of S-180 Tumors On the Morphology Of The Ovaries Andmentioning

confidence: 57%

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Sarcoma‑180 tumor affects the quality of oocytes in mice

et al. 2021

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Numerous factors can affect the quality of oocytes; however, the effects of cancer on the quality of oocytes and the underlying mechanisms remain unclear. In the present study, the effects of the sarcoma-180 (S-180) cell line on the quality of oocytes were investigated using S-180 tumor-bearing mice. In total, 42 female C57BL/6J mice were randomly divided into the tumor-bearing group and the control group, with 21 mice per group. The weight of the mice and ovaries were recorded, and blood glucose, serum insulin, lipopolysaccharide, triglyceride (TG) and total cholesterol (TC) levels were analyzed using the corresponding detection kits. Hematoxylin and eosin staining was performed to observe the pathological changes of the ovarian tissue, and reverse transcription-quantitative PCR (RT-qPCR) was used to analyze the expression levels of meiosis arrest female 1 (MARF1), SUMO-specific protease 7 (SENP7), aralkylamine N-acetyltransferase (AANAT), cell division cycle 25B and glycine-rich protein 3. The results of the present study revealed that the number of oocytes in the two groups of mice was similar; however, the number of abnormal oocytes was increased in the tumor-bearing group. The serum levels of TG and TC were significantly elevated in the tumor-bearing group compared with in the control group (P<0.01). Additionally, RT-qPCR analysis demonstrated that the expression levels of SENP7 were downregulated, while the expression levels of MARF1 and AANAT were upregulated in the ovaries of the tumor-bearing group compared with in the control group (P<0.01). In conclusion, the findings of the present study suggested that cancer may affect the reproductive system of mice and decrease the quality of oocytes by regulating the expression levels of reproduction-associated genes. These results provided novel insights into the reproductive ability of patients with cancer.

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Section: Discussioncontrasting

confidence: 68%

Section: Effects Of S-180 Tumors On the Morphology Of The Ovaries Andmentioning

confidence: 57%

Sarcoma‑180 tumor affects the quality of oocytes in mice

et al. 2021

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“…ROS levels in the oocytes were assessed in accordance with the manufacturer’s procedure [ 35 ]. MII oocytes subjected to different experimental conditions were incubated in α-MEM supplemented with 10 μM 2′,7′-dichlorodihydrofluorescein diacetate (Beyotime Institute of Biotechnology) at 37 °C for 30 min, then washed with PBS at least three times.…”

Section: Methodsmentioning

confidence: 99%

Effects of nicotinamide on follicular development and the quality of oocytes

Yang

Feng

et al. 2022

Reprod Biol Endocrinol

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Background Nicotinamide (NAM) is an important antioxidant, which is closely related to female fertility, but its role has not been clearly elucidated. The purpose of the present study was to investigate the effects of NAM on follicular development at different stages and the quality of oocytes. Methods The concentration of NAM in follicular fluid (FF) of 236 women undergoing in vitro fertilization (IVF) was ascertained by enzyme-linked immunosorbent assay (ELISA), and the correlation between NAM and clinical indexes was analyzed. During the in vitro maturation (IVM) of mice cumulus-oocyte complexes (COCs), different concentrations of NAM were added to check the maturation rate and fertilization rate. The reactive oxygen species (ROS) levels in the oocytes treated with different hydrogen peroxide (H2O2) and NAM were assessed. Immunofluorescence staining was performed to measure the proportion of abnormal spindles. Results The level of NAM in large follicles was significantly higher than that in small follicles. In mature FF, the NAM concentration was positively correlated with the rates of oocyte maturation and fertilization. Five mM NAM treatment during IVM increased maturation rate and fertilization rate in the oxidative stress model, and significantly reduced the increase of ROS levels induced by H2O2 in mice oocytes. Conclusions Higher levels of NAM in FF are associated with larger follicle development. The supplement of 5 mM NAM during IVM may improve mice oocyte quality, reducing damage caused by oxidative stress.

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“…Images were captured using a rotary laser confocal microscope (Dragonfly, Andor Technology). Oocytes with green fluorescence on the cytoplasmic membrane were considered early apoptotic oocytes ( He et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

“…The final step of oocyte development is oocyte maturation, which refers to when arrested oocytes resume meiosis and progress to metaphase II (MII). The process of oocyte maturation is particularly vulnerable to environmental pollutants and chemicals ( Rhind et al, 2010 ; He et al, 2019 ). The impairment of oocyte maturation may produce poor quality of oocytes, which is the common cause of infertility, miscarriage, and congenital genetic disease ( Nagaoka et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Aristolochic acid I exposure decreases oocyte quality

Zhang

et al. 2022

Front. Cell Dev. Biol.

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Oocyte quality is a determinant of a successful pregnancy. The final step of oocyte development is oocyte maturation, which is susceptible to environmental exposures. Aristolochic acids (AAs), widely existing in Aristolochia and Asarum plants that have been used in traditional medicine, can result in a smaller ovary and fewer superovulated oocytes after in vivo exposure to mice. However, whether AAs affect oocyte maturation and the underlying mechanism(s) are unclear. In this study, we focused on the effect of Aristolochic acid I (AAI), a major compound of AAs, on the maturation of in vitro cultured mouse oocytes. We showed that AAI exposure significantly decreased oocyte quality, including elevated aneuploidy, accompanied by aberrant chiasma patterns and spindle organization, and decreased first polar body extrusion and fertilization capability. Moreover, embryo development potential was also dramatically decreased. Further analyses revealed that AAI exposure significantly decreased mitochondrial membrane potential and ATP synthesis and increased the level of reactive oxygen species (ROS), implying impaired mitochondrial function. Insufficient ATP supply can cause aberrant spindle assembly and excessive ROS can cause premature loss of sister chromatid cohesion and thus alterations in chiasma patterns. Both aberrant spindles and changed chiasma patterns can contribute to chromosome misalignment and thus aneuploidy. Therefore, AAI exposure decreases oocyte quality probably via impairing mitochondrial function.

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Fenoxaprop‐ethyl affects mouse oocyte quality and the underlying mechanisms

Abstract: Our results indicate that FE has adverse effects on oocyte quality as assessed by maturation and fertilization potential, due to disrupted cytoskeletal integrality, and mitochondrial dysfunction leading to ROS accumulation, apoptosis and autophagy. © 2018 Society of Chemical Industry.

Cited by 15 publications

References 36 publications

Sarcoma‑180 tumor affects the quality of oocytes in mice

Sarcoma‑180 tumor affects the quality of oocytes in mice

Effects of nicotinamide on follicular development and the quality of oocytes

Aristolochic acid I exposure decreases oocyte quality

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