Fermentation and downstream processing of lipase from Aspergillus terreus

Gulati, Ruchi; Saxena, Rajendra Kumar; Gupta, Rani

doi:10.1016/s0032-9592(00)00201-6

Cited by 40 publications

(17 citation statements)

References 17 publications

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“…An increase in stirring speed up to 400 rpm resulted in a decrease in maximum lipase activity, assayed by the titrimetric method. These findings agree well with the results reported for Aspergillus terreus (Gulati et al, 2000), Rhizopus arrhizus (Elibol and Ozer, 2000) and Penicillium restrictum (Freire et al, 1997b).…”

Section: Discussionsupporting

confidence: 93%

“…For lipase production by Rhizopus arrhizus it has been reported that dissolved oxygen (DO) concentration is not the intrinsic factor affecting rates and yields (Elibol and Ozer, 2000). In addition, for lipase production with Aspergillus terreus, Gulati et al (2000) verified that the modelling of aerobic fermentations is more appropriate for oxygen transfer rate (OTR) than in terms of DO levels, as previously suggested by Chen et al (1999) for Acinetobacter radioresistens.…”

Section: Introductionsupporting

confidence: 59%

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Improvement of lipase production at different stirring speeds and oxygen levels

Alonso

Oliveira

Dellamora-Ortiz

et al. 2005

Braz. J. Chem. Eng.

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-Lipase production by a Brazilian wild strain of Yarrowia lipolytica at different stirring speeds and air flow rates was studied. The relationship among lipid consumption, cell growth and lipase production by this microorganism is presented. The most pronounced effect of oxygen on lipase production was determined by stirring speed. Maximum lipase activity was detected in the late stationary phase at 200 rpm and an air flow rate of 1-2 dm 3 /min (0.8-1.7 vvm) when the lipid source had been fully consumed. Higher stirring speeds resulted in mechanical and/or oxidative stress, while lower stirring speeds seemed to limit oxygen levels. An increase in the availability of oxygen at higher air flow rates led to faster lipid uptake and anticipation of enzyme release into the culture medium. The highest lipase production was obtained at 200 rpm and 1 dm 3 /min (0.8 vvm).

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Section: Discussionsupporting

confidence: 93%

Section: Introductionsupporting

confidence: 59%

Improvement of lipase production at different stirring speeds and oxygen levels

Alonso

Oliveira

Dellamora-Ortiz

et al. 2005

Braz. J. Chem. Eng.

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“…Previous investigations in our laboratory has shown Aspergillus carneus and Aspergillus terreus as potent lipase producers. We have reported certain results on production of these lipases [3,7,8]. In continuation of this, here we present some further studies carried out on process optimization of lipase from Aspergillus terreus by a statistical design method and its use in glyceride synthesis.…”

Section: Introductionmentioning

confidence: 88%

“…Other chemicals used were of analytical grade from Hi-Media (India). 8.0. Flasks containing production medium (50 ml in 250 ml flask) were inoculated with 5 9 10 7 spores per 50 ml (5-day-old slants used) and incubated at 37°C at 250 rpm for 96 h.…”

Section: Methodsmentioning

confidence: 99%

Optimization of Lipase Production from Aspergillus terreus by Response Surface Methodology and Its Potential for Synthesis of Partial Glycerides Under Solvent Free Conditions

et al. 2010

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Aspergillus terreus produces lipase 7.01 IU/ml in 96 h after optimization by one variable at a time method. Using the significant factors i.e. corn oil (A), sodium nitrate (B), casein (C), agitation rate (D) and incubation period (E) RSM was carried out resulting in 19.65 IU/ml from the combination ?1(A), -1(B), -1(C), ?1(D) and 0(E). The interactions between sodium nitrate, casein and agitation with corn oil were most significant. Scale up of production from 250 ml shake flask to 30 l bioreactor resulted in increased productivity of 0.52 IU/ml/h as against 0.2 IU/ ml/h obtained in shake flasks. This lipase could carryout solvent free synthesis of partial glycerides of oleic acid with 96% efficiency in 12 h.

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“…To a large extent, agitation increased lipase production and this is attributed to the fact that agitation increased oxygen transfer rate, increased surface area of contact with the media components and better dispersability of the oil substrate during fermentation under agitation condition [48,49]. Agitation rate within the range of 110 rpm to 160 rpm enhanced the lipase production by Pseudomonas gessardii.…”

Section: Effect Of Agitation Speedmentioning

confidence: 99%

Microbial Lipases: A Prospect for Biotechnological Industrial Catalysis for Green Products: A Review

Ugo¹,

Amara²,

Cn³

et al. 2017

Ferment Technol

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Microbes have been novel hosts for suitable industrial enzymes including lipases. With the rapid increase in biotechnological industries, there is need for improvement of bioproducts, enhance environmental safety and product yield. Microbes are qualified biological arsenal for the achievement of the above mentioned targets in the industrial sectors. Lipases as versatile biological catalyst has given a promising prospect in meeting the needs for most industries such as biodiesel, foods and drinks, leather, textile, detergents, pharmaceuticals and medicals. Catalytic importance of lipases includes hydrolysis, esterification and transesterification. Each of the mentioned reactions has their industrial applications. Lipases exhibit various properties, with respect to its source. Immobilization has made the use of microbial lipases attain its optimum performance and hence suitable for various reaction and the need to add flavor to the immobilization processes.

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Fermentation and downstream processing of lipase from Aspergillus terreus

Cited by 40 publications

References 17 publications

Improvement of lipase production at different stirring speeds and oxygen levels

Improvement of lipase production at different stirring speeds and oxygen levels

Optimization of Lipase Production from Aspergillus terreus by Response Surface Methodology and Its Potential for Synthesis of Partial Glycerides Under Solvent Free Conditions

Microbial Lipases: A Prospect for Biotechnological Industrial Catalysis for Green Products: A Review

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