Fermentation Blues: Analyzing the Microbiota of Traditional Indigo Vat Dyeing in Hunan, China

Li, Shan; Shi, Ying; Huang, Hui; Tong, Yan; Wu, Shaohua; Wang, Yuhua

doi:10.1128/spectrum.01663-22

Cited by 5 publications

(2 citation statements)

References 52 publications

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“…There are two sources of natural indigo dye, namely precipitated indigo (extracted indigo) and composted plants that contain indigo. Precipitated indigo has been used as a source of indigo dye in India, China, Southeast Asia, and Japan (Okinawa Prefecture) ( Toyama et al, 1978 ; Li et al, 2019 , 2022 ; Lopes et al, 2021 ). Since the microorganisms in the seeds are not associated with the precipitated indigo for fermentation, spontaneous initiation of indigo reduction can be time consuming.…”

Section: Introductionmentioning

confidence: 99%

Environmental factors contributing to the convergence of bacterial community structure during indigo reduction

Farjana

Tu²,

Furukawa³

et al. 2023

Front. Microbiol.

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Indigo is solubilized through the reducing action of the microbiota that occurs during alkaline fermentation of composted leaves of Polygonum tinctorium L. (sukumo). However, the environmental effects on the microbiota during this treatment, as well as the mechanisms underlying the microbial succession toward stable state remain unknown. In this study, physicochemical analyses and Illumina metagenomic sequencing was used to determine the impact pretreatment conditions on the subsequent initiation of bacterial community transition and their convergence, dyeing capacity and the environmental factors critical for indigo reducing state during aging of sukumo. The initial pretreatment conditions analyzed included 60°C tap water (heat treatment: batch 1), 25°C tap water (control; batch 2), 25°C wood ash extract (high pH; batch 3) and hot wood ash extract (heat and high pH; batch 4), coupled with successive addition of wheat bran from days 5 to 194. High pH had larger impact than heat treatment on the microbiota, producing more rapid transitional changes from days 1 to 2. Although the initial bacterial community composition and dyeing intensity differed during days 2–5, the microbiota appropriately converged to facilitate indigo reduction from day 7 in all the batches, with Alkaliphilus oremalandii, Amphibacillus, Alkalicella caledoniensis, Atopostipes suicloalis and Tissierellaceae core taxa contributing to the improvement of when the dyeing intensity. This convergence is attributed to the continuous maintenance of high pH (day 1 ~) and low redox potential (day 2~), along with the introduction of wheat bran at day 5 (day 5~). PICRUSt2 predictive function profiling revealed the enrichment of phosphotransferease system (PTS) and starch and sucrose metabolism subpathways key toward indigo reduction. Seven NAD(P)-dependent oxidoreductases KEGG orthologs correlating to the dyeing intensity was also identified, with Alkalihalobacillus macyae, Alkalicella caledoniensis, and Atopostipes suicloalis contributing significantly toward the initiation of indigo reduction in batch 3. During the ripening period, the staining intensity was maintained by continuous addition of wheat bran and the successive emergence of indigo-reducing bacteria that also contributed to material circulation in the system. The above results provide insight into the interaction of microbial system and environmental factors in sukumo fermentation.

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Section: Introductionmentioning

confidence: 99%

Environmental factors contributing to the convergence of bacterial community structure during indigo reduction

Farjana

Tu²,

Furukawa³

et al. 2023

Front. Microbiol.

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“…The business essentially involves the use of Vat dyes; these are anthraquinone and indigo-based dyes with carbonyl functional group 1 . Other countries known for the production or utilization of vat dyes include China and Pakistan 2 , 3 In actual fact textile industry play a key role in the economy of most countries 3 .…”

Section: Introductionmentioning

confidence: 99%

Effect of occupational exposure to vat-textile dyes on follicular and luteal hormones in female dye workers in Abeokuta, Nigeria

O Soyinka,

F Akinsanya,

A Odeyemi

et al. 2024

Afr H. Sci.

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Background: Some synthetic dyes used mainly in textile industries have been associated with endocrine disruption, resulting ininfertility, among other disorders. It is unknown if occupational exposure to Vat textile dyes among premenopausal dyers altershormonal levels. Objectives: We aimed at determining the probable effects of occupational exposure to Vat dyes on reproductive hormones offemale textile dyers in the follicular and luteal phases while relating this to age categories and duration of exposure. Methods: Thirty-three premenopausal Vat textile dyers at “Itoku”, Abeokuta, Nigeria, among a population of about 80 femaledyers were age and sex-matched with 55 non-exposed (control) female participants. Using semi-structured questionnaires, socio-demographic, occupational details and the LMP of participants were obtained. Serum samples were collected in follicularand luteal phases and assayed for female sex hormones using Enzyme Immunoassay. Mann-Whitney U and Z- statistic were usedfor comparison of the two groups. P-value < 0.05 was considered to be significant. Results: In the follicular phase, the result showed a lower mean FSH ranking (in age category ≤20 years) and higher (p<0.05)Estradiol ranking (in age category 31-40 years) in the exposed than the unexposed. Mean ranks of Progesterone and Estradiol inthe luteal phase (age category 31-40 years) were higher (p<0.05) in the exposed, while Estradiol (age category ≥41years) rankedlower (p<0.05). Prolactin demonstrated a significant inverse relationship with the duration of exposure. Conclusion: Occupational exposure to Vat dye among female dyers in Abeokuta is associated with some sex hormone disruptionwhich appears to be age and duration of exposure-related. Keywords: Follicular phase; luteal phase; dye workers; sex hormone; premenopausal.

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Microbial Communities Sustain Indigo Reduction Under Anaerobic Alkaline Conditions in the Indigo Fermentation Fluids

2024

Microbial Diversity in the Genomic Era

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Fermentation Blues: Analyzing the Microbiota of Traditional Indigo Vat Dyeing in Hunan, China

Cited by 5 publications

References 52 publications

Environmental factors contributing to the convergence of bacterial community structure during indigo reduction

Environmental factors contributing to the convergence of bacterial community structure during indigo reduction

Effect of occupational exposure to vat-textile dyes on follicular and luteal hormones in female dye workers in Abeokuta, Nigeria

Microbial Communities Sustain Indigo Reduction Under Anaerobic Alkaline Conditions in the Indigo Fermentation Fluids

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